PMID- 37999602
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231127
IS  - 2414-6366 (Electronic)
IS  - 2414-6366 (Linking)
VI  - 8
IP  - 11
DP  - 2023 Oct 26
TI  - The Role of GeneXpert((R)) for Tuberculosis Diagnostics in Brazil: An Examination 
      from a Historical and Epidemiological Perspective.
LID - 10.3390/tropicalmed8110483 [doi]
LID - 483
AB  - The rapid molecular test (RMT) performed on the GeneXpert((R)) system is widely 
      used as a control strategy and surveillance technique for tuberculosis (TB). In 
      the region of the Americas, TB incidence is slowly increasing owing to an upward 
      trend in Brazil, which is among the high TB-burden countries (HBCs), ranking in 
      the 19th position. In this context, we aimed to (i) describe the implementation 
      and history of RMT-TB (Xpert((R)) MTB/RIF and Xpert((R)) MTB/RIF Ultra) in Brazil; 
      (ii) to evaluate the national RMT laboratory distribution, TB, and resistance to 
      RIF detection by RMT; and (iii) to correlate these data with Brazilian TB 
      incidence. The quantitative data of Xpert((R)) MTB/RIF and Xpert((R)) MTB/RIF Ultra 
      assays performed in the pulmonary TB investigation from 2014 to 2020 were 
      provided by the Brazilian Ministry of Health. A spatial visualization using 
      ArcGIS software was performed. The Southeast region constituted about half of the 
      RMT laboratories-from 39.4% to 45.9% of the total value over the five regions. 
      Regarding the federal units, the Sao Paulo state alone represented from 20.2% to 
      34.1% (5.0 to 8.5 times the value) of RMT laboratories over the years observed. 
      There were significant differences (p < 0.0001) in the frequency of RMT 
      laboratories between all years of the historical series. There was an unequal 
      distribution of RMT laboratories between Brazilian regions and federal units. 
      This alerts us for the surveillance of rapid molecular detection of TB in 
      different parts of the country, with the possibility of improving the 
      distribution of tests in areas of higher incidence in order to achieve the level 
      of disease control recommended by national and worldwide authorities.
FAU - Iudice, Tirca Naiara da Silva
AU  - Iudice TNDS
AUID- ORCID: 0000-0002-1456-6158
AD  - Programa de Pos-Graduacao Biologia Parasitaria na Amazonia, Universidade do 
      Estado do Para, Belem-Para 66087-670, PA, Brazil.
FAU - da Conceicao, Marilia Lima
AU  - da Conceicao ML
AUID- ORCID: 0000-0002-3162-142X
AD  - Secao de Bacteriologia e Micologia, Instituto Evandro Chagas, Ananindeua 
      67030-000, PA, Brazil.
FAU - de Brito, Artemir Coelho
AU  - de Brito AC
AD  - Coordenacao-Geral de Vigilancia da Tuberculose, Micoses Endemicas e Micobacterias 
      Nao Tuberculosas-CGTM, Ministerio da Saude, Brasilia 70000-000, DF, Brazil.
FAU - de Souza, Nicole Menezes
AU  - de Souza NM
AD  - Coordenacao-Geral de Vigilancia da Tuberculose, Micoses Endemicas e Micobacterias 
      Nao Tuberculosas-CGTM, Ministerio da Saude, Brasilia 70000-000, DF, Brazil.
FAU - Mesquita, Cristal Ribeiro
AU  - Mesquita CR
AUID- ORCID: 0000-0002-1321-5719
AD  - Programa de Pos-Graduacao Biologia Parasitaria na Amazonia, Universidade do 
      Estado do Para, Belem-Para 66087-670, PA, Brazil.
FAU - Guimaraes, Ricardo Jose de Paula Souza E
AU  - Guimaraes RJPSE
AUID- ORCID: 0000-0002-5767-4765
AD  - Laboratorio de Geoprocessamento, Instituto Evandro Chagas, Ananindeua 67030-000, 
      PA, Brazil.
FAU - Furlaneto, Ismari Perini
AU  - Furlaneto IP
AUID- ORCID: 0000-0001-9941-0162
AD  - Curso de Medicina, Centro Universitario do Estado do Para, Belem 66087-662, PA, 
      Brazil.
FAU - Saboia, Alessandra de Souza
AU  - Saboia AS
AD  - Laboratorio de Bacteriologia e Bioensaios em Micobacterias, Instituto Nacional de 
      Infectologia Evandro Chagas, Fundacao Oswaldo Cruz, Manguinhos, Rio de Janeiro 
      21040-360, RJ, Brazil.
FAU - Lourenco, Maria Cristina da Silva
AU  - Lourenco MCDS
AD  - Laboratorio de Bacteriologia e Bioensaios em Micobacterias, Instituto Nacional de 
      Infectologia Evandro Chagas, Fundacao Oswaldo Cruz, Manguinhos, Rio de Janeiro 
      21040-360, RJ, Brazil.
FAU - Lima, Karla Valeria Batista
AU  - Lima KVB
AUID- ORCID: 0000-0001-5807-0392
AD  - Programa de Pos-Graduacao Biologia Parasitaria na Amazonia, Universidade do 
      Estado do Para, Belem-Para 66087-670, PA, Brazil.
AD  - Secao de Bacteriologia e Micologia, Instituto Evandro Chagas, Ananindeua 
      67030-000, PA, Brazil.
FAU - Conceicao, Emilyn Costa
AU  - Conceicao EC
AUID- ORCID: 0000-0002-7445-6620
AD  - Pos-Graduacao em Pesquisa Clinica e Doencas Infecciosas, Instituto Nacional de 
      Infectologia Evandro Chagas, Fundacao Oswaldo Cruz, Manguinhos, Rio de Janeiro 
      67030-000, RJ, Brazil.
AD  - Department of Science and Innovation-National Research Foundation Centre of 
      Excellence for Biomedical Tuberculosis Research, South African Medical Research 
      Council Centre for Tuberculosis Research, Division of Molecular Biology and Human 
      Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape 
      Town 7505, South Africa.
LA  - eng
PT  - Journal Article
DEP - 20231026
PL  - Switzerland
TA  - Trop Med Infect Dis
JT  - Tropical medicine and infectious disease
JID - 101709042
PMC - PMC10674801
OTO - NOTNLM
OT  - Brazil
OT  - Xpert MTB/RIF
OT  - Xpert MTB/RIF Ultra
OT  - rapid molecular test
OT  - tuberculosis
COIS- The authors declare no conflict of interest.
EDAT- 2023/11/24 12:43
MHDA- 2023/11/24 12:44
PMCR- 2023/10/26
CRDT- 2023/11/24 09:54
PHST- 2023/10/01 00:00 [received]
PHST- 2023/10/16 00:00 [revised]
PHST- 2023/10/20 00:00 [accepted]
PHST- 2023/11/24 12:44 [medline]
PHST- 2023/11/24 12:43 [pubmed]
PHST- 2023/11/24 09:54 [entrez]
PHST- 2023/10/26 00:00 [pmc-release]
AID - tropicalmed8110483 [pii]
AID - tropicalmed-08-00483 [pii]
AID - 10.3390/tropicalmed8110483 [doi]
PST - epublish
SO  - Trop Med Infect Dis. 2023 Oct 26;8(11):483. doi: 10.3390/tropicalmed8110483.