PMID- 38000575
OWN - NLM
STAT- MEDLINE
DCOM- 20240208
LR  - 20240208
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 258
IP  - Pt 1
DP  - 2024 Feb
TI  - Investigation of peanut allergen-procyanidin non-covalent interactions: Impact on 
      protein structure and in vitro allergenicity.
PG  - 128340
LID - S0141-8130(23)05239-X [pii]
LID - 10.1016/j.ijbiomac.2023.128340 [doi]
AB  - Interactions between plant polyphenols and food allergens may be a new way to 
      alleviate food allergies. The non-covalent interactions between the major 
      allergen from peanut (Ara h 2) with procyanidin dimer (PA2) were therefore 
      characterized using spectroscopic, thermodynamic, and molecular simulation 
      analyses. The main interaction between the Ara h 2 and PA2 was hydrogen bonding. 
      PA2 statically quenched the intrinsic fluorescence intensity and altered the 
      conformation of the Ara h 2, leading to a more disordered polypeptide structure 
      with a lower surface hydrophobicity. In addition, the in vitro allergenicity of 
      the Ara h 2-PA2 complex was investigated using enzyme-linked immunosorbent assay 
      (ELISA) kits. The immunoglobulin E (IgE) binding capacity of Ara h 2, as well as 
      the release of allergenic cytokines, decreased after interacting with PA2. When 
      the ratio of Ara h 2-to-PA2 was 1:50, the IgE binding capacity was reduced by 
      around 43 %. This study provides valuable insights into the non-covalent 
      interactions between Ara h 2 and PA2, as well as the potential mechanism of 
      action of the anti-allergic reaction caused by binding of the polyphenols to the 
      allergens.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Geng, Qin
AU  - Geng Q
AD  - State Key Laboratory of Food Science and Resources, Nanchang University, 
      Nanchang, China.
FAU - Zhang, Ying
AU  - Zhang Y
AD  - State Key Laboratory of Food Science and Resources, Nanchang University, 
      Nanchang, China.
FAU - McClements, David Julian
AU  - McClements DJ
AD  - Department of Food Science, University of Massachusetts, Amherst, MA 01003, USA.
FAU - Zhou, Wenlong
AU  - Zhou W
AD  - State Key Laboratory of Food Science and Resources, Nanchang University, 
      Nanchang, China.
FAU - Dai, Taotao
AU  - Dai T
AD  - State Key Laboratory of Food Science and Resources, Nanchang University, 
      Nanchang, China.
FAU - Wu, Zhihua
AU  - Wu Z
AD  - State Key Laboratory of Food Science and Resources, Nanchang University, 
      Nanchang, China; Sino-German Joint Research Institute, Nanchang University, 
      Nanchang, China. Electronic address: wuzhihua@ncu.edu.cn.
FAU - Chen, Hongbing
AU  - Chen H
AD  - State Key Laboratory of Food Science and Resources, Nanchang University, 
      Nanchang, China; Sino-German Joint Research Institute, Nanchang University, 
      Nanchang, China.
LA  - eng
PT  - Journal Article
DEP - 20231123
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Antigens, Plant)
RN  - 0 (Allergens)
RN  - 0 (Proanthocyanidins)
RN  - 0 (Glycoproteins)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 0 (Polyphenols)
RN  - 0 (Plant Proteins)
SB  - IM
MH  - Arachis/chemistry
MH  - Antigens, Plant/chemistry
MH  - Allergens/chemistry
MH  - *Proanthocyanidins/metabolism
MH  - Glycoproteins/chemistry
MH  - Immunoglobulin E/metabolism
MH  - Polyphenols/metabolism
MH  - *Peanut Hypersensitivity
MH  - Plant Proteins/chemistry
OTO - NOTNLM
OT  - Allergenicity
OT  - Ara h 2
OT  - Interaction
OT  - Procyanidin
OT  - Structure
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/11/25 07:42
MHDA- 2024/02/08 06:43
CRDT- 2023/11/24 19:28
PHST- 2023/10/12 00:00 [received]
PHST- 2023/11/19 00:00 [revised]
PHST- 2023/11/20 00:00 [accepted]
PHST- 2024/02/08 06:43 [medline]
PHST- 2023/11/25 07:42 [pubmed]
PHST- 2023/11/24 19:28 [entrez]
AID - S0141-8130(23)05239-X [pii]
AID - 10.1016/j.ijbiomac.2023.128340 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2024 Feb;258(Pt 1):128340. doi: 
      10.1016/j.ijbiomac.2023.128340. Epub 2023 Nov 23.