PMID- 38002299
OWN - NLM
STAT- MEDLINE
DCOM- 20231127
LR  - 20240104
IS  - 2218-273X (Electronic)
IS  - 2218-273X (Linking)
VI  - 13
IP  - 11
DP  - 2023 Nov 5
TI  - Preventive Effects of Collagen-Derived Dipeptide Prolyl-Hydroxyproline against 
      Dexamethasone-Induced Muscle Atrophy in Mouse C2C12 Skeletal Myotubes.
LID - 10.3390/biom13111617 [doi]
LID - 1617
AB  - Glucocorticoids, commonly used to manage inflammatory diseases, can induce muscle 
      atrophy by accelerating the breakdown of muscle proteins. This research delves 
      into the influence of Prolyl-hydroxyproline (Pro-Hyp), a collagen-derived 
      peptide, on muscle atrophy induced with dexamethasone (DEX), a synthetic 
      glucocorticoid, in mouse C2C12 skeletal myotubes. Exposure to DEX (10 muM) for 6 
      days resulted in a decrease in myotube diameter, along with elevated mRNA and 
      protein levels of two muscle-atrophy-related ubiquitin ligases, muscle atrophy 
      F-box (MAFbx, also known as atrogin-1) and muscle ring finger 1 (MuRF-1). 
      Remarkably, treatment with 0.1 mM of Pro-Hyp mitigated the reduction in myotube 
      thickness caused by DEX, while promoting the phosphorylation of Akt, mammalian 
      target of rapamycin (mTOR), and forkhead box O3a (Foxo3a). This led to the 
      inhibition of the upregulation of the ubiquitin ligases atrogin-1 and MuRF-1. 
      These findings indicate the potential significance of Pro-Hyp as a promising 
      therapeutic target for countering DEX-induced muscle atrophy.
FAU - Kimira, Yoshifumi
AU  - Kimira Y
AUID- ORCID: 0000-0001-5134-2519
AD  - Department of Clinical Dietetics and Human Nutrition, Faculty of Pharmacy and 
      Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado-shi 350-0295, 
      Japan.
FAU - Osawa, Konosuke
AU  - Osawa K
AD  - Department of Clinical Dietetics and Human Nutrition, Faculty of Pharmacy and 
      Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado-shi 350-0295, 
      Japan.
FAU - Osawa, Yoshihiro
AU  - Osawa Y
AD  - Department of Clinical Dietetics and Human Nutrition, Faculty of Pharmacy and 
      Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado-shi 350-0295, 
      Japan.
FAU - Mano, Hiroshi
AU  - Mano H
AD  - Department of Clinical Dietetics and Human Nutrition, Faculty of Pharmacy and 
      Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado-shi 350-0295, 
      Japan.
LA  - eng
GR  - 23K05095/Japan Society for the Promotion of Science/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231105
PL  - Switzerland
TA  - Biomolecules
JT  - Biomolecules
JID - 101596414
RN  - 0 (prolyl-4-hydroxyproline)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 0 (Glucocorticoids)
RN  - 0 (Dipeptides)
RN  - EC 6.- (Ligases)
RN  - 0 (Ubiquitins)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Muscle, Skeletal/metabolism
MH  - *Dexamethasone/pharmacology/therapeutic use
MH  - Muscle Fibers, Skeletal/metabolism
MH  - Muscular Atrophy/chemically induced/drug therapy/prevention & control
MH  - Glucocorticoids/pharmacology/therapeutic use
MH  - Dipeptides/pharmacology
MH  - Ligases/metabolism
MH  - Ubiquitins/metabolism
MH  - Ubiquitin-Protein Ligases/metabolism
MH  - Mammals/metabolism
PMC - PMC10669392
OTO - NOTNLM
OT  - Prolyl-hydroxyproline
OT  - collagen-derived peptide
OT  - mouse C2C12 skeletal myotubes
OT  - muscle atrophy
OT  - ubiquitin ligases
COIS- The authors declare no conflict of interest.
EDAT- 2023/11/25 12:42
MHDA- 2023/11/27 12:42
PMCR- 2023/11/05
CRDT- 2023/11/25 01:09
PHST- 2023/08/17 00:00 [received]
PHST- 2023/11/02 00:00 [revised]
PHST- 2023/11/03 00:00 [accepted]
PHST- 2023/11/27 12:42 [medline]
PHST- 2023/11/25 12:42 [pubmed]
PHST- 2023/11/25 01:09 [entrez]
PHST- 2023/11/05 00:00 [pmc-release]
AID - biom13111617 [pii]
AID - biomolecules-13-01617 [pii]
AID - 10.3390/biom13111617 [doi]
PST - epublish
SO  - Biomolecules. 2023 Nov 5;13(11):1617. doi: 10.3390/biom13111617.