PMID- 38003194
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231128
IS  - 2076-2615 (Print)
IS  - 2076-2615 (Electronic)
IS  - 2076-2615 (Linking)
VI  - 13
IP  - 22
DP  - 2023 Nov 19
TI  - Mild Intermittent Cold Stimulation Affects Cardiac Substance Metabolism via the 
      Neuroendocrine Pathway in Broilers.
LID - 10.3390/ani13223577 [doi]
LID - 3577
AB  - This study aimed to investigate the impact of cold adaptation on the 
      neuroendocrine and cardiac substance metabolism pathways in broilers. The 
      broilers were divided into the control group (CC), cold adaptation group (C3), 
      and cold-stressed group (C9), and experimental period was divided into the 
      training period (d 1-35), recovery period (d 36-43), and cold stress period (d 
      43-44). During the training period, the CC group was reared at ambient 
      temperature, while C3 and C9 groups were reared at 3  degrees C and 9  degrees C lower than the 
      ambient temperature, respectively, for 5 h/d at 1 d intervals. During the 
      recovery period, all the groups were maintained at 20  degrees C. Lastly, during the cold 
      stress period, the groups were divided into two sub-groups, and each sub-group 
      was placed at 10  degrees C for 12 h (Y12) or 24 h (Y24) for acute cold stimulation. The 
      blood, hypothalamic, and cardiac tissues samples were obtained from all the 
      groups during the training, recovery, and acute stress periods. The results 
      revealed that the transcription of calcium voltage-gated channel subunit alpha 1 
      C (CACNAIC) was increased in the hypothalamic tissues of the C3 group (p < 0.05). 
      Moreover, compared to the CC group, the serum norepinephrine (NE) was increased 
      in the C9 group (p < 0.05), but insulin (INS) was decreased in the C9 group (p < 
      0.05). In addition, the transcription of the phosphoinositide-3 kinase (PI3K), 
      protein kinase B (Akt), mammalian target of rapamycin (mTOR), SREBP1c, FASN, 
      ACC1, and SCD genes was down-regulated in the C3 and C9 groups (p < 0.05); 
      however, their expression increased in the C3 and C9 groups after acute cold 
      stimulation (p < 0.05). Compared to the CC group, the transcription of forkhead 
      box O1 (FoxO1), PEPCK, G6Pase, GLUT1, HK1, PFK, and LDHB genes was up-regulated 
      in the C3 and C9 groups (p < 0.05. Furthermore, compared to the CC and C9 groups, 
      the protein and mRNA expressions of heat shock protein (HSP) 70 and HSP90 were 
      significantly increased in the C3 group (p < 0.05). These results indicate that 
      intermittent cold training can enhance cold stress tolerance in broilers by 
      regulating their neuroendocrine and cardiac substance metabolism pathways.
FAU - Liu, Yuanyuan
AU  - Liu Y
AD  - College of Life Science, Northeast Agricultural University, Harbin 150030, China.
FAU - Xing, Lu
AU  - Xing L
AD  - College of Life Science, Northeast Agricultural University, Harbin 150030, China.
FAU - Zhang, Yong
AU  - Zhang Y
AD  - College of Life Science, Northeast Agricultural University, Harbin 150030, China.
FAU - Liu, Xiaotao
AU  - Liu X
AD  - College of Life Science, Northeast Agricultural University, Harbin 150030, China.
FAU - Li, Tingting
AU  - Li T
AD  - College of Life Science, Northeast Agricultural University, Harbin 150030, China.
FAU - Zhang, Shijie
AU  - Zhang S
AD  - College of Life Science, Northeast Agricultural University, Harbin 150030, China.
FAU - Wei, Haidong
AU  - Wei H
AUID- ORCID: 0000-0002-6623-5502
AD  - College of Life Science, Northeast Agricultural University, Harbin 150030, China.
FAU - Li, Jianhong
AU  - Li J
AUID- ORCID: 0000-0002-7736-0333
AD  - College of Life Science, Northeast Agricultural University, Harbin 150030, China.
AD  - Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and 
      Rural Affairs, Harbin 150030, China.
LA  - eng
GR  - 32172785/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20231119
PL  - Switzerland
TA  - Animals (Basel)
JT  - Animals : an open access journal from MDPI
JID - 101635614
PMC - PMC10668735
OTO - NOTNLM
OT  - cold adaptation
OT  - cold stress
OT  - glucose metabolism
OT  - lipid metabolism
OT  - neuroendocrine
COIS- The authors declare no conflict of interest.
EDAT- 2023/11/25 12:47
MHDA- 2023/11/25 12:48
PMCR- 2023/11/19
CRDT- 2023/11/25 01:14
PHST- 2023/09/04 00:00 [received]
PHST- 2023/11/08 00:00 [revised]
PHST- 2023/11/13 00:00 [accepted]
PHST- 2023/11/25 12:48 [medline]
PHST- 2023/11/25 12:47 [pubmed]
PHST- 2023/11/25 01:14 [entrez]
PHST- 2023/11/19 00:00 [pmc-release]
AID - ani13223577 [pii]
AID - animals-13-03577 [pii]
AID - 10.3390/ani13223577 [doi]
PST - epublish
SO  - Animals (Basel). 2023 Nov 19;13(22):3577. doi: 10.3390/ani13223577.