PMID- 38003291
OWN - NLM
STAT- MEDLINE
DCOM- 20231129
LR  - 20231129
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 22
DP  - 2023 Nov 8
TI  - Dipeptidyl Peptidase 4 Stimulation Induces Adipogenesis-Related Gene Expression 
      of Adipose Stromal Cells.
LID - 10.3390/ijms242216101 [doi]
LID - 16101
AB  - Adipogenesis has emerged as a new therapeutic target for regulating metabolism 
      and achieving anti-inflammatory and anti-atherosclerotic effects via the release 
      of adiponectin. However, at present, the effects and mechanism of action of 
      dipeptidyl peptidase 4 (DPP4) stimulation on adiponectin production and 
      adipogenesis have not been clarified. Here, we investigated the effects of DPP4 
      stimulation with monocyte chemoattractant protein-1 (MCP-1) on platelet-derived 
      growth factor receptor alpha (PDGFRalpha) expression in adipose tissue and blood 
      adiponectin levels. Stromal vascular fractions (SVFs) purified from human 
      subcutaneous adipose tissue and inguinal adipose tissue of obese and diabetic 
      (Lepr(db/db)) mice were treated with 50 ng of MCP-1 and plasma from control 
      (Lepr(+/+)) mice supplemented with 10 ng or 50 ng of MCP-1. Treatment of SVFs 
      from human subcutaneous adipose tissues with 50 ng of MCP-1 significantly 
      increased AdipoQ, DPP4, peroxisome proliferator-activated receptor gamma (PPARgamma), 
      fatty-acid-binding protein (FABP4), and SERBF1 mRNA expression. 
      MCP-1-supplemented plasma increased adiponectin, CCAAT-Enhancer-binding protein 
      alpha (C/EBPalpha), DPP4, IL-33, and PDGFRalpha mRNA expression and adiponectin and DPP4 
      protein expression, while decreasing the expression of IL-10 mRNA in SVFs 
      compared with the levels in the plasma treatment group. MCP-1-supplemented plasma 
      was shown to increase PPARgamma, PPARgamma2, adiponectin, DPP4, and FABP4 and decrease 
      IL-10 mRNA expression in PDGFRalpha cells from adipose tissue. Meanwhile, 
      MCP-1-supplemented plasma increased MCP-1, PDGFRalpha, TNFalpha, adiponectin, and IL-1beta 
      and decreased IL-10 and FOXP3 mRNA expression in DPP4 cells. Moreover, the 
      injection of MCP-1-supplemented plasma into adipose tissue increased the 
      proportion of DPP4(+) cells among PDGFRalpha(+) cells from adipose tissue and plasma 
      adiponectin levels of Lepr(db/db) mice compared with the levels in the plasma 
      injection group. Our results demonstrate that DPP4(+) cells are important adipose 
      progenitor cells. Stimulation of DPP4 with MCP-1 increases adipogenesis-related 
      gene expression and the population of DPP4(+) cells among PDGFRalpha(+) cells in SVFs 
      and blood adiponectin levels. DPP4 stimulation could be a novel therapy to 
      increase local adipogenesis and systemic adiponectin levels.
FAU - Lai, Hsiao-Chi
AU  - Lai HC
AUID- ORCID: 0000-0002-8332-8970
AD  - Department of Surgery, Kaohsiung Veterans General Hospital, No. 386, Ta-Chung 1st 
      Road, Kaohsiung 813, Taiwan.
AD  - Institute of Emergency and Critical Care Medicine, National Yang Ming Chiao Tung 
      University, No. 155, Sec. 2, Linong Street, Taipei 112, Taiwan.
FAU - Chen, Pei-Hsuan
AU  - Chen PH
AD  - Department of Surgery, Kaohsiung Veterans General Hospital, No. 386, Ta-Chung 1st 
      Road, Kaohsiung 813, Taiwan.
AD  - Institute of Emergency and Critical Care Medicine, National Yang Ming Chiao Tung 
      University, No. 155, Sec. 2, Linong Street, Taipei 112, Taiwan.
FAU - Tang, Chia-Hua
AU  - Tang CH
AD  - Department of Surgery, Kaohsiung Veterans General Hospital, No. 386, Ta-Chung 1st 
      Road, Kaohsiung 813, Taiwan.
FAU - Chen, Lee-Wei
AU  - Chen LW
AUID- ORCID: 0000-0002-3583-1248
AD  - Department of Surgery, Kaohsiung Veterans General Hospital, No. 386, Ta-Chung 1st 
      Road, Kaohsiung 813, Taiwan.
AD  - Institute of Emergency and Critical Care Medicine, National Yang Ming Chiao Tung 
      University, No. 155, Sec. 2, Linong Street, Taipei 112, Taiwan.
AD  - Department of Biological Sciences, National Sun Yat-Sen University, No. 70, 
      Lien-Hai Road, Kaohsiung 804, Taiwan.
LA  - eng
GR  - KSVGH112-082/Kaohsiung Veterans General Hospital/
PT  - Journal Article
DEP - 20231108
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Adiponectin)
RN  - EC 3.4.14.5 (Dipeptidyl Peptidase 4)
RN  - 130068-27-8 (Interleukin-10)
RN  - 0 (PPAR gamma)
RN  - EC 2.7.10.1 (Receptor, Platelet-Derived Growth Factor alpha)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.14.5 (Dpp4 protein, mouse)
SB  - IM
MH  - Animals
MH  - Humans
MH  - Mice
MH  - *Adipogenesis/genetics
MH  - *Adiponectin/metabolism
MH  - Dipeptidyl Peptidase 4/genetics
MH  - Gene Expression
MH  - Interleukin-10/genetics
MH  - PPAR gamma/metabolism
MH  - Receptor, Platelet-Derived Growth Factor alpha/genetics
MH  - RNA, Messenger/metabolism
MH  - Stromal Cells/metabolism
PMC - PMC10671339
OTO - NOTNLM
OT  - FOXP3
OT  - IL-10
OT  - PDGFRalpha
OT  - adipogenesis
OT  - adipose
COIS- The authors declare no conflict of interest.
EDAT- 2023/11/25 12:42
MHDA- 2023/11/27 16:18
PMCR- 2023/11/08
CRDT- 2023/11/25 01:15
PHST- 2023/09/17 00:00 [received]
PHST- 2023/11/01 00:00 [revised]
PHST- 2023/11/04 00:00 [accepted]
PHST- 2023/11/27 16:18 [medline]
PHST- 2023/11/25 12:42 [pubmed]
PHST- 2023/11/25 01:15 [entrez]
PHST- 2023/11/08 00:00 [pmc-release]
AID - ijms242216101 [pii]
AID - ijms-24-16101 [pii]
AID - 10.3390/ijms242216101 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Nov 8;24(22):16101. doi: 10.3390/ijms242216101.