PMID- 38003599
OWN - NLM
STAT- MEDLINE
DCOM- 20231127
LR  - 20231127
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 22
DP  - 2023 Nov 16
TI  - Anti-Proteolytic Peptide R7I Protects the Intestinal Barrier and Alleviates Fatty 
      Acid Malabsorption in Salmonella typhimurium-Infected Mice.
LID - 10.3390/ijms242216409 [doi]
LID - 16409
AB  - With a wide range of hosts, environmental adaptation, and antibiotic resistance, 
      Salmonella typhimurium is one of the most common causes of food poisoning in the 
      world. Infection with Salmonella typhimurium not only results in intestinal 
      inflammation but also damages the intestinal barrier and interferes with the 
      host's ability to absorb nutrients. It is imperative to find alternatives to 
      antibiotics for eradicating bacteria, reducing intestinal damage, and 
      reestablishing nutrient absorption, especially given that antibiotics are 
      currently prohibited. This research aims to understand the protective role of 
      anti-proteolytic peptide R7I on the gut in the setting of Salmonella typhimurium 
      infection and its impact on nutritional absorption, maybe offering an alternative 
      to antibiotics for bacterial killing. The findings demonstrated that R7I reduced 
      the production of inflammatory factors, including IL-6, TNF-alpha, and L-1beta in the 
      jejunum and decreased the expression of genes like TLR4 and NF-kappaB in the jejunum 
      (p < 0.05). R7I enhanced antioxidant capacity and preserved the 
      antioxidant/pro-oxidant balance in the jejunum (p < 0.05). R7I also normalized 
      intestinal shape and restored tight junction protein expression. Fatty acid 
      binding protein 2 (FABP2) and fatty acid transport protein 4 (FATP4) expression 
      in the jejunum was restored by R7I. In addition, serum-free fatty acids and lipid 
      metabolites were significantly higher in the R7I group than in the control group 
      (p < 0.05). Overall, the anti-enzyme peptide R7I maintained the healthy state of 
      the intestine and alleviated the abnormal fatty acid absorption caused by 
      bacterial infection.
FAU - Su, Yunzhe
AU  - Su Y
AD  - College of Animal Science and Technology, Northeast Agricultural University, 
      Harbin 150030, China.
FAU - Sun, Taotao
AU  - Sun T
AD  - College of Animal Science and Technology, Northeast Agricultural University, 
      Harbin 150030, China.
FAU - Gao, Junhan
AU  - Gao J
AD  - College of Animal Science and Technology, Northeast Agricultural University, 
      Harbin 150030, China.
FAU - Zhang, Chenxu
AU  - Zhang C
AD  - College of Animal Science and Technology, Northeast Agricultural University, 
      Harbin 150030, China.
FAU - Liu, Xuesheng
AU  - Liu X
AD  - College of Animal Science and Technology, Northeast Agricultural University, 
      Harbin 150030, China.
FAU - Bi, Chongpeng
AU  - Bi C
AD  - College of Animal Science and Technology, Northeast Agricultural University, 
      Harbin 150030, China.
FAU - Wang, Jiajun
AU  - Wang J
AUID- ORCID: 0000-0002-2086-2105
AD  - College of Animal Science and Technology, Northeast Agricultural University, 
      Harbin 150030, China.
FAU - Shan, Anshan
AU  - Shan A
AD  - College of Animal Science and Technology, Northeast Agricultural University, 
      Harbin 150030, China.
LA  - eng
GR  - 32030101/National Natural Science Foundation of China/
GR  - 32272914/National Natural Science Foundation of China/
GR  - 2022YFD1300700/National Key R&D Program of China/
GR  - YQ2020C009/Natural Science Foundation of Heilongjiang Province/
PT  - Journal Article
DEP - 20231116
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Fatty Acids)
RN  - 0 (Antioxidants)
RN  - 0 (Peptides)
RN  - EC 3.4.- (Peptide Hydrolases)
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Salmonella typhimurium
MH  - Fatty Acids
MH  - Antioxidants
MH  - *Salmonella Infections/drug therapy
MH  - Peptides
MH  - Peptide Hydrolases
MH  - Anti-Bacterial Agents
PMC - PMC10670956
OTO - NOTNLM
OT  - Salmonella typhimurium
OT  - anti-proteolytic peptide
OT  - fatty acid absorption
OT  - inflammation
OT  - intestinal barrier
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/11/25 12:45
MHDA- 2023/11/27 12:42
PMCR- 2023/11/16
CRDT- 2023/11/25 01:17
PHST- 2023/10/09 00:00 [received]
PHST- 2023/10/26 00:00 [revised]
PHST- 2023/11/06 00:00 [accepted]
PHST- 2023/11/27 12:42 [medline]
PHST- 2023/11/25 12:45 [pubmed]
PHST- 2023/11/25 01:17 [entrez]
PHST- 2023/11/16 00:00 [pmc-release]
AID - ijms242216409 [pii]
AID - ijms-24-16409 [pii]
AID - 10.3390/ijms242216409 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Nov 16;24(22):16409. doi: 10.3390/ijms242216409.