PMID- 38005924 OWN - NLM STAT- MEDLINE DCOM- 20231127 LR - 20231127 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 15 IP - 11 DP - 2023 Nov 11 TI - Pathogenicity Studies of NADC34-like Porcine Reproductive and Respiratory Syndrome Virus LNSY-GY and NADC30-like Porcine Reproductive and Respiratory Syndrome Virus GXGG-8011 in Piglets. LID - 10.3390/v15112247 [doi] LID - 2247 AB - The porcine reproductive and respiratory syndrome virus (PRRSV) has caused significant economic losses to the swine industry. The U.S., China, and Peru have reported NADC30-like or NADC34-like PRRSV-infected piglets, which have been identified as the cause of a significant number of abortions in clinics. Although the pathogenicity of NADC30-like PRRSV and NADC34-like PRRSV in piglets exhibits significant variability globally, studies on their pathogenicity in China are limited. In this study, the animal experiments showed that within 8-14 days post-infection, both piglets infected with NADC30-like PRRSV GXGG-8011 and those infected with NADC34-like PRRSV LNSY-GY exhibited significant weight loss compared to the control piglets. Additionally, the viremia of the LNSY-GY persisted for 28 days, while the viremia of piglets infected with the GXGG-8011 lasted for 17 days. Similarly, the duration of viral shedding through the fecal-oral route after the LNSY-GY infection was longer than that observed after the GXGG-8011 infection. Furthermore, post-infection, both the LNSY-GY and GXGG-8011 led to pronounced histopathological lesions in the lungs of piglets, including interstitial pneumonia and notable viral colonization. However, the antibody production in the LNSY-GY-infected group occurred earlier than that in the GXGG-8011-infected group. Our research findings indicate that LNSY-GY is a mildly pathogenic strain in piglets, whereas we speculate that the GXGG-8011 might be a highly pathogenic strain. FAU - Zhu, Hechao AU - Zhu H AD - National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. AD - The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. FAU - Wei, Liuqing AU - Wei L AD - National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. AD - The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. FAU - Liu, Xiangzu AU - Liu X AD - National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. AD - The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. FAU - Liu, Shudan AU - Liu S AD - National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. AD - The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. FAU - Chen, Huanchun AU - Chen H AD - National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. AD - The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. FAU - Chen, Pin AU - Chen P AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. FAU - Li, Xiangmin AU - Li X AD - National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. AD - The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. FAU - Qian, Ping AU - Qian P AD - National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. AD - The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. LA - eng GR - IRIFH202209/the Yingzi Tech & Huazhong Agricultural University Intelligent Research Institute of Food Health/ GR - 2022YFD1800800/the National Key R&D Program of China/ GR - YXNM-R&D2023-08/the Creative Program on Key Research Project of Guangxi Yangxiang Agriculture and Husbandry Co., LTD/ PT - Journal Article DEP - 20231111 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 SB - IM MH - Animals MH - Swine MH - *Porcine respiratory and reproductive syndrome virus MH - *Porcine Reproductive and Respiratory Syndrome MH - Virulence MH - Viremia MH - Genome, Viral MH - Phylogeny MH - China PMC - PMC10674415 OTO - NOTNLM OT - NADC30-like OT - NADC34-like OT - PRRSV OT - pathogenicity COIS- The authors declare no conflict of interest. EDAT- 2023/11/25 12:47 MHDA- 2023/11/27 12:42 PMCR- 2023/11/11 CRDT- 2023/11/25 01:30 PHST- 2023/10/15 00:00 [received] PHST- 2023/11/08 00:00 [revised] PHST- 2023/11/09 00:00 [accepted] PHST- 2023/11/27 12:42 [medline] PHST- 2023/11/25 12:47 [pubmed] PHST- 2023/11/25 01:30 [entrez] PHST- 2023/11/11 00:00 [pmc-release] AID - v15112247 [pii] AID - viruses-15-02247 [pii] AID - 10.3390/v15112247 [doi] PST - epublish SO - Viruses. 2023 Nov 11;15(11):2247. doi: 10.3390/v15112247.