PMID- 38007158
OWN - NLM
STAT- MEDLINE
DCOM- 20240102
LR  - 20240106
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 895
DP  - 2024 Feb 15
TI  - Molecular mechanism of microRNA-mediated hypoglycemic effect of whole grain 
      highland barley.
PG  - 148021
LID - S0378-1119(23)00862-4 [pii]
LID - 10.1016/j.gene.2023.148021 [doi]
AB  - As a non-coding RNA, microRNA (miRNA) has been proven to play an important role 
      in the development and progression of type 2 diabetes mellitus (T2DM). Highland 
      barley is a whole grain from the Tibetan areas of China. Our previous studies 
      have demonstrated its hypoglycemic effect. To further explore the underlining 
      molecular mechanism, we investigated the effect of highland barley intervention 
      on liver miRNA expression profiles in diabetic mice. Our results showed that ten 
      differentially expressed miRNA among different groups were identified and their 
      target genes were predicted. Remarkably, many glycometabolism-associated genes, 
      including Foxo3, Nras, Rptor, Igf1r, Tsc2 and Braf, were negatively regulated by 
      miR-122-5p, miR-503-5p, miR-455-5p and miR-210-3p, respectively. Pathway 
      enrichment analysis revealed these target genes were mainly involved in AMPK, 
      MAPK and FOXO signaling pathways. Thereby, these miRNA and mRNA were validated 
      using qRT-PCR, and the results were consistent with the small RNA-seq and 
      expectations. Highland barley could regulate the MAPK, AMPK, and FOXO signaling 
      pathways by regulating critical miRNA-mRNA pairs, e.x. miR-210-3p-Tsc2/Braf, 
      miR-122-5p-Foxo3, and miR-455-5p-Igf1r, thereby improving blood glucose 
      metabolism in diabetic mice. The present study preliminarily explored the 
      hypoglycaemic effects of highland barley based on transcriptomics, and more 
      detailed and in-depth studies on this topic are needed in the future.
CI  - Copyright (c) 2023. Published by Elsevier B.V.
FAU - Qin, Mengyuan
AU  - Qin M
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing 
      Engineering and Technology Research Center of Food Additives, National Grain 
      Industry Highland Barley Deep Processing Technology Innovation Center, Beijing 
      Technology and Business University, Beijing 100048, China.
FAU - Ren, Xin
AU  - Ren X
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing 
      Engineering and Technology Research Center of Food Additives, National Grain 
      Industry Highland Barley Deep Processing Technology Innovation Center, Beijing 
      Technology and Business University, Beijing 100048, China.
FAU - Zhang, Min
AU  - Zhang M
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing 
      Engineering and Technology Research Center of Food Additives, National Grain 
      Industry Highland Barley Deep Processing Technology Innovation Center, Beijing 
      Technology and Business University, Beijing 100048, China. Electronic address: 
      zmin@th.btbu.edu.cn.
FAU - Chen, Zenglong
AU  - Chen Z
AD  - State Key Laboratory of Integrated Management of Pest Insects and Rodents, 
      Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
FAU - Shen, Jing
AU  - Shen J
AD  - Ningjin County Market Supervision Administration, Dezhou, Shandong 253400, China.
LA  - eng
PT  - Journal Article
DEP - 20231123
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (MicroRNAs)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins B-raf)
RN  - 0 (Hypoglycemic Agents)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *MicroRNAs/metabolism
MH  - Proto-Oncogene Proteins B-raf
MH  - *Hordeum/genetics
MH  - Hypoglycemic Agents
MH  - *Diabetes Mellitus, Type 2
MH  - AMP-Activated Protein Kinases
MH  - *Diabetes Mellitus, Experimental/genetics
MH  - Whole Grains
MH  - RNA, Messenger
OTO - NOTNLM
OT  - Glucose metabolism
OT  - Highland barley
OT  - Target gene
OT  - microRNA
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/11/26 07:44
MHDA- 2024/01/02 11:46
CRDT- 2023/11/25 19:30
PHST- 2023/08/18 00:00 [received]
PHST- 2023/11/08 00:00 [revised]
PHST- 2023/11/21 00:00 [accepted]
PHST- 2024/01/02 11:46 [medline]
PHST- 2023/11/26 07:44 [pubmed]
PHST- 2023/11/25 19:30 [entrez]
AID - S0378-1119(23)00862-4 [pii]
AID - 10.1016/j.gene.2023.148021 [doi]
PST - ppublish
SO  - Gene. 2024 Feb 15;895:148021. doi: 10.1016/j.gene.2023.148021. Epub 2023 Nov 23.