PMID- 38015303
OWN - NLM
STAT- Publisher
LR  - 20231128
IS  - 1559-1182 (Electronic)
IS  - 0893-7648 (Linking)
DP  - 2023 Nov 28
TI  - Tauroursodeoxycholic Acid Inhibited Apoptosis and Oxidative Stress in 
      H(2)O(2)-Induced BMSC Death via Modulating the Nrf-2 Signaling Pathway: the 
      Therapeutic Implications in a Rat Model of Spinal Cord Injury.
LID - 10.1007/s12035-023-03754-5 [doi]
AB  - Spinal cord injury (SCI) is a prevalent and significant injury to the central 
      nervous system, resulting in severe consequences. This injury is characterized by 
      motor, sensory, and excretory dysfunctions below the affected spinal segment. 
      Transplantation of bone marrow mesenchymal stem cells (BMSCs) has emerged as a 
      potential treatment for SCI. However, the low survival as well as the 
      differentiation rates of BMSCs within the spinal cord microenvironment 
      significantly limit their therapeutic efficiency. Tauroursodeoxycholic acid 
      (TUDCA), an active ingredient found in bear bile, has demonstrated its 
      neuroprotective, antioxidant, and antiapoptotic effects on SCI. Thus, the present 
      study was aimed to study the possible benefits of combining TUDCA with BMSC 
      transplantation using an animal model of SCI. The results showed that TUDCA 
      significantly enhanced BMSC viability and reduced apoptosis (assessed by Annexin 
      V-FITC, TUNEL, Bax, Bcl-2, and Caspase-3) as well as oxidative stress (assessed 
      by ROS, GSH, SOD, and MDA) both in vitro and in vivo. Additionally, TUDCA 
      accelerated tissue regeneration (assessed by HE, Nissl, MAP2, MBP, TUJ1, and 
      GFAP) and improved functional recovery (assessed by BBB score) following BMSC 
      transplantation in SCI. These effects were mediated via the Nrf-2 signaling 
      pathway, as evidenced by the upregulation of Nrf-2, NQO-1, and HO-1 expression 
      levels. Overall, these results indicate that TUDCA could serve as a valuable 
      adjunct to BMSC transplantation therapy for SCI, potentially enhancing its 
      therapeutic efficacy.
CI  - (c) 2023. The Author(s).
FAU - Weng, Jiaxian
AU  - Weng J
AD  - School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, 
      Guangzhou, 510006, Guangdong, China.
AD  - Lingnan Medical Research Center of Guangzhou University of Chinese Medicine, 
      Guangzhou, 510405, China.
AD  - Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
FAU - Wang, Le
AU  - Wang L
AD  - Department of Spine Surgery, the First Affiliated Hospital of Sun Yat-Sen 
      University, Guangdong Provincial Key Laboratory of Orthopedics and Traumatology, 
      Guangzhou, 510080, Guangdong, China.
FAU - Wang, Kai
AU  - Wang K
AD  - Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
FAU - Su, Haitao
AU  - Su H
AD  - State Key Laboratory of Dampness Syndrome of Chinese Medicine, Department of 
      Orthopedic Surgery,, The Second Affiliated Hospital of Guangzhou University of 
      Chinese Medicine, Guangzhou, 510120, China.
FAU - Luo, Dan
AU  - Luo D
AD  - Lingnan Medical Research Center of Guangzhou University of Chinese Medicine, 
      Guangzhou, 510405, China.
AD  - State Key Laboratory of Dampness Syndrome of Chinese Medicine, Department of 
      Orthopedic Surgery,, The Second Affiliated Hospital of Guangzhou University of 
      Chinese Medicine, Guangzhou, 510120, China.
FAU - Yang, Haimei
AU  - Yang H
AD  - Lingnan Medical Research Center of Guangzhou University of Chinese Medicine, 
      Guangzhou, 510405, China.
AD  - Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
FAU - Wen, Yaqian
AU  - Wen Y
AD  - Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
FAU - Wu, Qiduan
AU  - Wu Q
AD  - The First Affiliated Hospital of Chinese Medicine, Guangzhou University of 
      Chinese Medicine, Guangzhou, 510405, China. wuqiduan@126.com.
FAU - Li, Xing
AU  - Li X
AUID- ORCID: 0000-0002-5676-8962
AD  - Lingnan Medical Research Center of Guangzhou University of Chinese Medicine, 
      Guangzhou, 510405, China. liqixi723@126.com.
AD  - Guangzhou University of Chinese Medicine, Guangzhou, 510405, China. 
      liqixi723@126.com.
AD  - State Key Laboratory of Dampness Syndrome of Chinese Medicine, Department of 
      Orthopedic Surgery,, The Second Affiliated Hospital of Guangzhou University of 
      Chinese Medicine, Guangzhou, 510120, China. liqixi723@126.com.
LA  - eng
GR  - No. 82004384/Natural Science Foundation of China/
GR  - No.82102583/Natural Science Foundation of China/
PT  - Journal Article
DEP - 20231128
PL  - United States
TA  - Mol Neurobiol
JT  - Molecular neurobiology
JID - 8900963
SB  - IM
OTO - NOTNLM
OT  - Apoptosis
OT  - Bone marrow mesenchymal stem cells
OT  - Oxidative stress
OT  - Spinal cord injury
OT  - TUDCA
EDAT- 2023/11/28 12:42
MHDA- 2023/11/28 12:42
CRDT- 2023/11/28 11:09
PHST- 2023/06/26 00:00 [received]
PHST- 2023/10/28 00:00 [accepted]
PHST- 2023/11/28 12:42 [medline]
PHST- 2023/11/28 12:42 [pubmed]
PHST- 2023/11/28 11:09 [entrez]
AID - 10.1007/s12035-023-03754-5 [pii]
AID - 10.1007/s12035-023-03754-5 [doi]
PST - aheadofprint
SO  - Mol Neurobiol. 2023 Nov 28. doi: 10.1007/s12035-023-03754-5.