PMID- 38015348
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231201
IS  - 2662-8651 (Electronic)
IS  - 2662-8651 (Linking)
VI  - 4
IP  - 1
DP  - 2023 Nov 28
TI  - Osteopontin enhances the effect of treadmill training and promotes functional 
      recovery after spinal cord injury.
PG  - 44
LID - 10.1186/s43556-023-00154-y [doi]
LID - 44
AB  - In this study, we examined the combined impact of osteopontin (OPN) and treadmill 
      training on mice with spinal cord injury (SCI). OPN was overexpressed by 
      injecting AAV9-SPP1-GFP into the sensorimotor cortex, followed by a left 
      incomplete C5 crush injury two weeks later. Mice (Ex or Ex + OPN group) were 
      trained at 50% maximum running speed for 8 weeks. To analyze the effects, we used 
      biotinylated dextran amine (BDA) for tracing the corticospinal tract (CST) and 
      performed Western blotting and immunohistochemical methods to assess the 
      activation of the mammalian target of rapamycin (mTOR). We also examined axonal 
      regeneration and conducted behavioral tests to measure functional recovery. The 
      results demonstrated that treadmill training promoted the expression of 
      neurotrophic factors such as brain-derived neurotrophic factor (BNDF) and 
      insulin-like growth factor I (IGF-1) and activated mTOR signaling. OPN amplified 
      the effect of treadmill training on activating mTOR signaling indicated by 
      upregulated phosphorylation of ribosomal protein S6 kinase (S6). The combination 
      of OPN and exercise further promoted functional recovery and facilitated limited 
      CST axonal regeneration which did not occur with treadmill training and OPN 
      treatment alone. These findings indicate that OPN enhances the effects of 
      treadmill training in the treatment of SCI and offer new therapeutic insights for 
      spinal cord injury.
CI  - (c) 2023. The Author(s).
FAU - Wang, Yunhang
AU  - Wang Y
AD  - Department of Rehabilitation Medicine, The Second Affiliated Hospital of 
      Chongqing Medical University, Chongqing, 400010, China.
AD  - Department of Rehabilitation, Zhejiang University School of Medicine Second 
      Affiliated Hospital, 88 Jiefang Road, Hangzhou, Zhejiang, 310009, China.
FAU - Su, Hong
AU  - Su H
AD  - Department of Rehabilitation Medicine, The Second Affiliated Hospital of 
      Chongqing Medical University, Chongqing, 400010, China.
FAU - Zhong, Juan
AU  - Zhong J
AD  - Department of Rehabilitation Medicine, The Second Affiliated Hospital of 
      Chongqing Medical University, Chongqing, 400010, China.
FAU - Zhan, Zuxiong
AU  - Zhan Z
AD  - Department of Rehabilitation Medicine, The Second Affiliated Hospital of 
      Chongqing Medical University, Chongqing, 400010, China.
FAU - Zhao, Qin
AU  - Zhao Q
AD  - Department of Rehabilitation Medicine, The Second Affiliated Hospital of 
      Chongqing Medical University, Chongqing, 400010, China.
FAU - Liu, Yuan
AU  - Liu Y
AD  - State Key Laboratory of Trauma, Burns and Combined Injury, Department of Special 
      War Wound, Daping Hospital, Army Medical University, Chongqing, 400042, China.
FAU - Li, Sen
AU  - Li S
AD  - State Key Laboratory of Trauma, Burns and Combined Injury, Department of Special 
      War Wound, Daping Hospital, Army Medical University, Chongqing, 400042, China.
FAU - Wang, Haiyan
AU  - Wang H
AD  - State Key Laboratory of Trauma, Burns and Combined Injury, Department of Special 
      War Wound, Daping Hospital, Army Medical University, Chongqing, 400042, China.
FAU - Yang, Ce
AU  - Yang C
AD  - State Key Laboratory of Trauma, Burns and Combined Injury, Department of Special 
      War Wound, Daping Hospital, Army Medical University, Chongqing, 400042, China.
FAU - Yu, Lehua
AU  - Yu L
AD  - Department of Rehabilitation Medicine, The Second Affiliated Hospital of 
      Chongqing Medical University, Chongqing, 400010, China.
FAU - Tan, Botao
AU  - Tan B
AD  - Department of Rehabilitation Medicine, The Second Affiliated Hospital of 
      Chongqing Medical University, Chongqing, 400010, China. 303518@cqmu.edu.cn.
FAU - Yin, Ying
AU  - Yin Y
AD  - Department of Rehabilitation Medicine, The Second Affiliated Hospital of 
      Chongqing Medical University, Chongqing, 400010, China. 300735@cqmu.edu.cn.
LA  - eng
GR  - 81702221/National Natural Science Foundation of China/
GR  - 82002377/National Natural Science Foundation of China/
GR  - cstc2020jcyj-msxm0651/Natural Science Foundation of Chongqing/
GR  - cstc2019jcyj-msxmX0195/Natural science Foundation of Chongqing/
GR  - 2020MSXM108/Chongqing Municipal Science and Technology Bureau and Health 
      Commission/
PT  - Journal Article
DEP - 20231128
PL  - Singapore
TA  - Mol Biomed
JT  - Molecular biomedicine
JID - 9918283581406676
PMC - PMC10684450
OTO - NOTNLM
OT  - Functional recovery
OT  - Osteopontin
OT  - Spinal cord injury
OT  - Treadmill training
COIS- There are no conflicts of interest to disclose in the submission of this 
      manuscript. On behalf of all the authors, I affirm that the research presented 
      here is original, has not been previously published, and is not under 
      consideration for publication elsewhere, whether in full or in part. Furthermore, 
      all the named authors have thoroughly reviewed and provided their approval for 
      this enclosed manuscript.
EDAT- 2023/11/28 12:42
MHDA- 2023/11/28 12:43
PMCR- 2023/11/28
CRDT- 2023/11/28 11:13
PHST- 2023/07/08 00:00 [received]
PHST- 2023/11/05 00:00 [accepted]
PHST- 2023/11/28 12:43 [medline]
PHST- 2023/11/28 12:42 [pubmed]
PHST- 2023/11/28 11:13 [entrez]
PHST- 2023/11/28 00:00 [pmc-release]
AID - 10.1186/s43556-023-00154-y [pii]
AID - 154 [pii]
AID - 10.1186/s43556-023-00154-y [doi]
PST - epublish
SO  - Mol Biomed. 2023 Nov 28;4(1):44. doi: 10.1186/s43556-023-00154-y.