PMID- 38021186
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231201
IS  - 2248-2997 (Print)
IS  - 2248-2997 (Electronic)
IS  - 2248-2997 (Linking)
VI  - 12
IP  - 4
DP  - 2022 Dec
TI  - Prevalence of multidrug-resistant Gram-negative bacteria from blood cultures and 
      rapid detection of beta-lactamase-encoding genes by multiplex PCR assay.
PG  - 434-443
LID - 10.18683/germs.2022.1349 [doi]
AB  - INTRODUCTION: This study aimed to determine the prevalence of multidrug-resistant 
      Gram-negative bacteria (GNB) from blood cultures in a tertiary-care hospital and 
      the multiplex PCR assay's ability to detect resistance genes. METHODS: A total of 
      388 GNB isolates obtained from hospitalized patients between November 2019 and 
      November 2021 were included in the study. Antimicrobial susceptibility testing 
      was done by VITEK 2 system and broth microdilution method. 
      Beta-lactamase-encoding genes were detected by multiplex PCR assays, 
      BioFire-Blood Culture Identification 2 (BCID2) panel (bioMerieux, France). 
      Extended-spectrum beta-lactamases (ESBLs) were detected phenotypically with VITEK 
      AST-GN71 card (bioMerieux, France). The isolates of GNB were classified into 
      multidrug-resistant, extensively-drug-resistant, and pandrug-resistant 
      categories, and their prevalence and distribution in different wards, including 
      coronavirus diseases 2019 (COVID-19) intensive care units (ICU), were calculated. 
      RESULTS: Results revealed that all isolates of Acinetobacter baumannii and 
      Pseudomonas aeruginosa were multidrug-resistant as well as 91.6% of Enterobacter 
      cloacae, 80.6% of Proteus mirabilis, and 76.1% of Klebsiella pneumoniae, 
      respectively. In fermentative bacteria, bla(OXA-48-like) (58.1%), bla(NDM) 
      (16.1%), bla(KPC) (9.7%) and bla(VIM) (6.5%) genes were detected. More than half 
      of Enterobacter cloacae (58.3%) and Klebsiella pneumoniae (53.7%) produced ESBLs. 
      Among non-fermenters, the bla(NDM) gene was carried by 55% of Pseudomonas 
      aeruginosa and 19.5% of Acinetobacter baumannii. In the COVID-19 ICU, 
      Acinetobacter baumannii was the most common isolate (86.1%). CONCLUSIONS: This 
      study revealed high proportions of multidrug-resistant blood isolates and various 
      underlying resistance genes in Gram-negative strains. The BCID2 panel seems to be 
      helpful for the detection of the most prevalent resistance genes of fermentative 
      bacteria.
CI  - GERMS.
FAU - Zornic, Sanja
AU  - Zornic S
AD  - MD, University Clinical Center Kragujevac, Department of Microbiology, Zmaj 
      Jovina 30, 34000 Kragujevac, Serbia.
FAU - Lukovic, Bojana
AU  - Lukovic B
AD  - MD, PhD, Academy of Applied Studies Belgrade, College of Health Sciences, Cara 
      Dusana 254, 11080 Belgrade, Serbia.
FAU - Petrovic, Ivana
AU  - Petrovic I
AD  - MD, University Clinical Center Kragujevac, Department of Microbiology, Zmaj 
      Jovina 30, 34000 Kragujevac, Serbia.
FAU - Jencic, Aleksandra
AU  - Jencic A
AD  - MD, Public Health Institute Pozarevac, Department of Microbiology, Jovana 
      Serbanovica 14, 12000 Pozarevac, Serbia.
LA  - eng
PT  - Journal Article
DEP - 20221231
PL  - Romania
TA  - Germs
JT  - Germs
JID - 101596099
PMC - PMC10660225
OTO - NOTNLM
OT  - Blood culture
OT  - Gram-negative bacteria
OT  - beta-lactamases
OT  - carbapenemases
OT  - multidrug-resistant
COIS- Conflicts of interest: All authors - none to declare.
EDAT- 2023/11/29 18:42
MHDA- 2023/11/29 18:43
PMCR- 2022/12/31
CRDT- 2023/11/29 14:59
PHST- 2022/08/03 00:00 [received]
PHST- 2022/10/17 00:00 [revised]
PHST- 2022/12/29 00:00 [accepted]
PHST- 2023/11/29 18:43 [medline]
PHST- 2023/11/29 18:42 [pubmed]
PHST- 2023/11/29 14:59 [entrez]
PHST- 2022/12/31 00:00 [pmc-release]
AID - germs.2022.1349 [pii]
AID - 10.18683/germs.2022.1349 [doi]
PST - epublish
SO  - Germs. 2022 Dec 31;12(4):434-443. doi: 10.18683/germs.2022.1349. eCollection 2022 
      Dec.