PMID- 38022592
OWN - NLM
STAT- MEDLINE
DCOM- 20231201
LR  - 20240401
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 14
DP  - 2023
TI  - Molecular and cellular characterization of immunity conferred by lactobacilli 
      against necrotic enteritis in chickens.
PG  - 1301980
LID - 10.3389/fimmu.2023.1301980 [doi]
LID - 1301980
AB  - Necrotic enteritis is an important enteric disease of poultry that can be 
      controlled with in-feed antibiotics. However, with the concerns over 
      antimicrobial resistance, there is an increased interest in the use of 
      alternatives. Probiotics are one of the alternatives that have gained 
      considerable attention due to their antimicrobial and immunomodulatory 
      activities. Therefore, in the present study, we evaluated the effects of two 
      different Lactobacillus species alone or as a cocktail on prevention of necrotic 
      enteritis. Day-old male broiler chickens were divided into five groups and on 
      days 1, 8, 15, and 22, birds in groups 2 and 3 received 1x10(8) colony forming 
      units (CFU) of L. johnsonii and L. reuteri, respectively. Group 4 received 
      probiotic cocktails containing both bacteria (10(8) CFU/bird) and the negative 
      and positive control groups did not receive any lactobacilli. Starting on day 23 
      post-hatch, birds in all groups (except the negative control group) were orally 
      challenged twice per day with 3x10(8) CFU of a pathogenic C. perfringens strain 
      for 3 days. Tissue and cecal samples were collected before and after challenge to 
      assess gene expression, lymphocyte subsets determination, and microbiome 
      analysis. On day 26 of age, lesion scoring was performed. The results 
      demonstrated that the group that received the lactobacilli cocktail had 
      significantly reduced lesion scores compared to the positive control group. In 
      addition, the expression of interleukin (IL)-12 in the jejunum and CXC motif 
      chemokine ligand 8 (CXCL8), IL-13, and IL-17 in the ileum were downregulated in 
      the group that received the lactobacilli cocktail when compared to the positive 
      control. Treating chickens with the lactobacilli cocktail prior to challenge 
      enhanced the percentage of CD3(-)CD8(+) cells and Bu-1(+)IgY(+) B cells in the 
      ileum and increased the frequency of monocyte/macrophages, CD3(-)CD8(+) cells, 
      Bu-1(+)IgM(+), and Bu-1(+)IgY(+) B cells in the jejunum. Treatment with the 
      lactobacilli cocktail reduced the relative expression of Gamma-Protobacteria and 
      Firmicutes compared to the positive control group. In conclusion, the results 
      presented here suggest that treatment with the lactobacilli cocktail containing 
      L. johnsonii and L. reuteri reduced necrotic enteritis lesions in the small 
      intestine of chickens, possibly through the modulation of immune responses.
CI  - Copyright (c) 2023 Alizadeh, Shojadoost, Boodhoo, Raj and Sharif.
FAU - Alizadeh, Mohammadali
AU  - Alizadeh M
AD  - Department of Pathobiology, Ontario Veterinary College, University of Guelph, 
      Guelph, ON, Canada.
FAU - Shojadoost, Bahram
AU  - Shojadoost B
AD  - Ceva Animal Health Inc., Guelph, ON, Canada.
FAU - Boodhoo, Nitish
AU  - Boodhoo N
AD  - Department of Pathobiology, Ontario Veterinary College, University of Guelph, 
      Guelph, ON, Canada.
FAU - Raj, Sugandha
AU  - Raj S
AD  - Department of Pathobiology, Ontario Veterinary College, University of Guelph, 
      Guelph, ON, Canada.
FAU - Sharif, Shayan
AU  - Sharif S
AD  - Department of Pathobiology, Ontario Veterinary College, University of Guelph, 
      Guelph, ON, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231107
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Animals
MH  - Male
MH  - *Clostridium Infections/prevention & control/veterinary/microbiology
MH  - *Enteritis/prevention & control/veterinary/microbiology
MH  - Chickens/microbiology
MH  - Lactobacillus
MH  - Clostridium perfringens/physiology
MH  - Anti-Bacterial Agents
PMC - PMC10662302
OTO - NOTNLM
OT  - chicken
OT  - cytokine
OT  - lactobacilli
OT  - lymphocyte
OT  - microbiome
OT  - necrotic enteritis
COIS- Author BS was employed by the company "CEVA Animal Health". The remaining authors 
      declare that the research was conducted in the absence of any commercial of 
      financial relationship that could be construed as potential conflict of interest.
EDAT- 2023/11/29 18:41
MHDA- 2023/12/01 06:45
PMCR- 2023/01/01
CRDT- 2023/11/29 15:27
PHST- 2023/09/25 00:00 [received]
PHST- 2023/10/23 00:00 [accepted]
PHST- 2023/12/01 06:45 [medline]
PHST- 2023/11/29 18:41 [pubmed]
PHST- 2023/11/29 15:27 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2023.1301980 [doi]
PST - epublish
SO  - Front Immunol. 2023 Nov 7;14:1301980. doi: 10.3389/fimmu.2023.1301980. 
      eCollection 2023.