PMID- 38023485
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231201
IS  - 2662-6810 (Print)
IS  - 2052-7276 (Electronic)
IS  - 2052-7276 (Linking)
VI  - 10
IP  - 11
DP  - 2023 Nov
TI  - PoWRKY71 is involved in Paeonia ostii resistance to drought stress by directly 
      regulating light-harvesting chlorophyll a/b-binding 151 gene.
PG  - uhad194
LID - 10.1093/hr/uhad194 [doi]
LID - uhad194
AB  - Although the functions of WRKY transcription factors in drought resistance are 
      well known, their regulatory mechanisms in response to drought by stabilising 
      photosynthesis remain unclear. Here, a differentially expressed PoWRKY71 gene 
      that was highly expressed in drought-treated Paeonia ostii leaves was identified 
      through transcriptome analysis. PoWRKY71 positively responded to drought stress 
      with significantly enhanced expression patterns and overexpressing PoWRKY71 in 
      tobacco greatly improved plant tolerance to drought stress, whereas silencing 
      PoWRKY71 in P. ostii resulted in a drought-intolerant phenotype. Furthermore, 
      lower chlorophyll contents, photosynthesis, and inhibited expression of 
      photosynthesis-related light-harvesting chlorophyll a/b-binding 151 (CAB151) gene 
      were found in PoWRKY71-silenced P. ostii. Meanwhile, a homologous system 
      indicated that drought treatment increased PoCAB151 promoter activity. 
      Interactive assays revealed that PoWRKY71 directly bound on the W-box element of 
      PoCAB151 promoter and activated its transcription. In addition, PoCAB151 
      overexpressing plants demonstrated increased drought tolerance, together with 
      significantly higher chlorophyll contents and photosynthesis, whereas these 
      indices were dramatically lower in PoCAB151-silenced P. ostii. The above results 
      indicated that PoWRKY71 activated the expression of PoCAB151, thus stabilising 
      photosynthesis via regulating chloroplast homeostasis and chlorophyll content in 
      P. ostii under drought stress. This study reveals a novel drought-resistance 
      mechanism in plants and provides a feasible strategy for improving plant drought 
      resistance via stabilising photosynthesis.
CI  - (c) The Author(s) 2023. Published by Oxford University Press on behalf of Nanjing 
      Agricultural University.
FAU - Luan, Yuting
AU  - Luan Y
AD  - College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 
      225009, China.
FAU - Chen, Zijie
AU  - Chen Z
AD  - College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 
      225009, China.
FAU - Fang, Ziwen
AU  - Fang Z
AD  - College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 
      225009, China.
FAU - Huang, Xingqi
AU  - Huang X
AD  - Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.
FAU - Zhao, Daqiu
AU  - Zhao D
AD  - College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 
      225009, China.
FAU - Tao, Jun
AU  - Tao J
AD  - College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 
      225009, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.
LA  - eng
PT  - Journal Article
DEP - 20230925
PL  - England
TA  - Hortic Res
JT  - Horticulture research
JID - 101655540
PMC - PMC10673652
COIS- The authors declare no conflict of interest.
EDAT- 2023/11/29 18:42
MHDA- 2023/11/29 18:43
PMCR- 2023/09/25
CRDT- 2023/11/29 15:49
PHST- 2023/06/16 00:00 [received]
PHST- 2023/09/17 00:00 [accepted]
PHST- 2023/11/29 18:43 [medline]
PHST- 2023/11/29 18:42 [pubmed]
PHST- 2023/11/29 15:49 [entrez]
PHST- 2023/09/25 00:00 [pmc-release]
AID - uhad194 [pii]
AID - 10.1093/hr/uhad194 [doi]
PST - epublish
SO  - Hortic Res. 2023 Sep 25;10(11):uhad194. doi: 10.1093/hr/uhad194. eCollection 2023 
      Nov.