PMID- 38026466
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231201
IS  - 1178-7090 (Print)
IS  - 1178-7090 (Electronic)
IS  - 1178-7090 (Linking)
VI  - 16
DP  - 2023
TI  - Acupuncture Relieves Cervical Spondylosis Radiculopathy by Regulating Spinal 
      Microglia Activation Through MAPK Signaling Pathway in Rats.
PG  - 3945-3960
LID - 10.2147/JPR.S419927 [doi]
AB  - PURPOSE: Local acupuncture has been found to have a good analgesic effect in rats 
      with cervical spondylosis radiculopathy (CSR), but it lacks a regulatory effect 
      on traditional Chinese medicine syndrome types of CSR. We proposed "Invigorating 
      Qi and activating Blood" (IQAB) acupuncture, compared with Fenbid, and local 
      electroacupuncture (LEA), to observe whether it has advantages in the protection 
      of the CSR rat model and to elucidate its mechanism through the MAPK 
      (mitogen-activated protein kinase) signaling pathway. MATERIALS AND METHODS: Male 
      Sprague-Dawley rats were randomly divided into six groups: control, sham, model, 
      Fenbid, LEA, and IQAB. The CSR model was induced by inserting nylon sutures to 
      compress the C(4)-T(1) nerve root. The Fenbid group was treated with ibuprofen 
      sustained-release capsules (15 mg/kg.d, ig). The LEA group received 
      electroacupuncture at both C(5) and C(7) EX-B2 once a day. The IQAB group 
      received acupuncture at both ST36 and BL17 based on the LEA group's intervention. 
      Mechanical allodynia and gait, morphological changes in the spinal cord, IL-6 and 
      TNF-alpha levels, MAPKs phosphorylation ratio, monocyte chemoattractant protein-1 
      (MCP-1) levels in the spinal cord, and the expression of p-p38 in the spinal cord 
      and its colocalization with neurons and glial cell activation markers were 
      detected. RESULTS: Mechanical allodynia, gait disorder, edema, reduced 
      Nissl-positive cell numbers, and increased IL-6 and TNF-alpha levels in the spinal 
      cord were observed in CSR rats. IQAB significantly alleviated these changes, and 
      the effects were generally comparable to those of Fenbid. Meanwhile, the 
      phosphorylation ratios of p38 and extracellular regulated protein kinase (ERK), 
      co-expression of p-p38 with neuron/microglia, and MCP-1 levels in the spinal cord 
      were markedly down-regulated by IQAB compared with those in CSR model rats. 
      CONCLUSION: IQAB reduced p38-activation-related microglia activation and MCP-1 
      levels, thus alleviating pathological changes, inflammation levels in the local 
      spinal cord, and pain behavior of CSR.
CI  - (c) 2023 Shi et al.
FAU - Shi, Tianyu
AU  - Shi T
AUID- ORCID: 0000-0002-1169-7371
AD  - School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese 
      Medicine, Beijing, People's Republic of China.
FAU - Liu, Yitian
AU  - Liu Y
AD  - School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese 
      Medicine, Beijing, People's Republic of China.
FAU - Ji, Bo
AU  - Ji B
AD  - School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese 
      Medicine, Beijing, People's Republic of China.
FAU - Wang, Jiajia
AU  - Wang J
AD  - School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese 
      Medicine, Beijing, People's Republic of China.
FAU - Ge, Yunpeng
AU  - Ge Y
AD  - School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese 
      Medicine, Beijing, People's Republic of China.
FAU - Fang, Yang
AU  - Fang Y
AD  - School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese 
      Medicine, Beijing, People's Republic of China.
FAU - Xie, Yana
AU  - Xie Y
AD  - School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese 
      Medicine, Beijing, People's Republic of China.
FAU - Xiao, Hongli
AU  - Xiao H
AD  - School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese 
      Medicine, Beijing, People's Republic of China.
FAU - Wu, Le
AU  - Wu L
AD  - School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese 
      Medicine, Beijing, People's Republic of China.
FAU - Wang, Yifei
AU  - Wang Y
AD  - School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese 
      Medicine, Beijing, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20231120
PL  - New Zealand
TA  - J Pain Res
JT  - Journal of pain research
JID - 101540514
PMC - PMC10674675
OTO - NOTNLM
OT  - MCP-1
OT  - analgesia
OT  - chemokine
OT  - inflammatory cytokine
OT  - mitogen activated protein kinases
COIS- The authors report no conflicts of interest in this work.
EDAT- 2023/11/29 18:41
MHDA- 2023/11/29 18:42
PMCR- 2023/11/20
CRDT- 2023/11/29 16:53
PHST- 2023/05/04 00:00 [received]
PHST- 2023/11/09 00:00 [accepted]
PHST- 2023/11/29 18:42 [medline]
PHST- 2023/11/29 18:41 [pubmed]
PHST- 2023/11/29 16:53 [entrez]
PHST- 2023/11/20 00:00 [pmc-release]
AID - 419927 [pii]
AID - 10.2147/JPR.S419927 [doi]
PST - epublish
SO  - J Pain Res. 2023 Nov 20;16:3945-3960. doi: 10.2147/JPR.S419927. eCollection 2023.