PMID- 38026878
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231201
IS  - 2296-4185 (Print)
IS  - 2296-4185 (Electronic)
IS  - 2296-4185 (Linking)
VI  - 11
DP  - 2023
TI  - Biomimetic electrospun nanofibrous scaffold for tissue engineering: preparation, 
      optimization by design of experiments (DOE), in-vitro and in-vivo 
      characterization.
PG  - 1288539
LID - 10.3389/fbioe.2023.1288539 [doi]
LID - 1288539
AB  - Electrospinning is a versatile method for fabrication of precised nanofibrous 
      materials for various biomedical application including tissue engineering and 
      drug delivery. This research is aimed to fabricate the PVP/PVA nanofiber scaffold 
      by novel electrospinning technique and to investigate the impact of process 
      parameters (flow rate, voltage and distance) and polymer concentration/solvent 
      combinations influence on properties of electrospun nanofibers. The in-vitro and 
      in-vivo degradation studies were performed to evaluate the potential of 
      electrospun PVP/PVA as a tissue engineering scaffold. The solvents used for 
      electrospinning of PVP/PVA nanofibers were ethanol and 90% acetic acid, optimized 
      with central composite design via Design Expert software. NF-2 and NF-35 were 
      selected as optimised nanofiber formulation in acetic acid and ethanol, and their 
      characterization showed diameter of 150-400 nm, tensile strength of 18.3 and 
      13.1 MPa, respectively. XRD data revealed the amorphous nature, and exhibited 
      hydrophilicity (contact angles: 67.89 degrees  and 58.31 degrees  for NF-2 and NF-35). Swelling 
      and in-vitro degradability studies displayed extended water retention as well as 
      delayed degradation. FTIR analysis confirmed solvent-independent interactions. 
      Additionally, hemolysis and in-vitro cytotoxicity studies revealed the non-toxic 
      nature of fabricated scaffolds on RBCs and L929 fibroblast cells. Subcutaneous 
      rat implantation assessed tissue response, month-long biodegradation, and 
      biocompatibility through histological analysis of surrounding tissue. Due to its 
      excellent biocompatibility, this porous PVP/PVA nanofiber has great potential for 
      biomedical applications.
CI  - Copyright (c) 2023 Anjum, Li, Arya, Ali, Alarifi, Yulin, Hengtong, Rajinikanth and 
      Ao.
FAU - Anjum, Shabnam
AU  - Anjum S
AD  - Department of Tissue Engineering, School of Intelligent Medicine, China Medical 
      University, Shenyang, Liaoning, China.
AD  - NMPA Key Laboratory for Quality Research and Control of Tissue Regenerative 
      Biomaterial, National Engineering Research Centre for Biomaterials, Institute of 
      Regulatory Science for Medical Device, Sichuan University, Chengdu, Sichuan, 
      China.
FAU - Li, Ting
AU  - Li T
AD  - Department of Laboratory Medicine, Shengjing Hospital of China Medical 
      University, Shenyang, Liaoning, China.
FAU - Arya, Dilip Kumar
AU  - Arya DK
AD  - Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University, 
      Vidya Vihar, Lucknow, India.
FAU - Ali, Daoud
AU  - Ali D
AD  - Department of Zoology, College of Science, King Saud University, Riyadh, Saudi 
      Arabia.
FAU - Alarifi, Saud
AU  - Alarifi S
AD  - Department of Zoology, College of Science, King Saud University, Riyadh, Saudi 
      Arabia.
FAU - Yulin, Wang
AU  - Yulin W
AD  - NMPA Key Laboratory for Quality Research and Control of Tissue Regenerative 
      Biomaterial, National Engineering Research Centre for Biomaterials, Institute of 
      Regulatory Science for Medical Device, Sichuan University, Chengdu, Sichuan, 
      China.
FAU - Hengtong, Zhang
AU  - Hengtong Z
AD  - NMPA Key Laboratory for Quality Research and Control of Tissue Regenerative 
      Biomaterial, National Engineering Research Centre for Biomaterials, Institute of 
      Regulatory Science for Medical Device, Sichuan University, Chengdu, Sichuan, 
      China.
FAU - Rajinikanth, P S
AU  - Rajinikanth PS
AD  - Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University, 
      Vidya Vihar, Lucknow, India.
FAU - Ao, Qiang
AU  - Ao Q
AD  - Department of Tissue Engineering, School of Intelligent Medicine, China Medical 
      University, Shenyang, Liaoning, China.
AD  - NMPA Key Laboratory for Quality Research and Control of Tissue Regenerative 
      Biomaterial, National Engineering Research Centre for Biomaterials, Institute of 
      Regulatory Science for Medical Device, Sichuan University, Chengdu, Sichuan, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20231031
PL  - Switzerland
TA  - Front Bioeng Biotechnol
JT  - Frontiers in bioengineering and biotechnology
JID - 101632513
PMC - PMC10646156
OTO - NOTNLM
OT  - PVA
OT  - PVP
OT  - electrospinning
OT  - subcutaneous implant
OT  - tissue engineering
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/11/29 18:41
MHDA- 2023/11/29 18:42
PMCR- 2023/01/01
CRDT- 2023/11/29 16:58
PHST- 2023/09/06 00:00 [received]
PHST- 2023/10/16 00:00 [accepted]
PHST- 2023/11/29 18:42 [medline]
PHST- 2023/11/29 18:41 [pubmed]
PHST- 2023/11/29 16:58 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 1288539 [pii]
AID - 10.3389/fbioe.2023.1288539 [doi]
PST - epublish
SO  - Front Bioeng Biotechnol. 2023 Oct 31;11:1288539. doi: 10.3389/fbioe.2023.1288539. 
      eCollection 2023.