PMID- 38029390
OWN - NLM
STAT- MEDLINE
DCOM- 20231216
LR  - 20231216
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 71
IP  - 49
DP  - 2023 Dec 13
TI  - Fluorescence Polarization Assay Based on a New Recognition Motif QepA for the 
      One-Step Detection of Fluoroquinolones in Eggs.
PG  - 19749-19759
LID - 10.1021/acs.jafc.3c03526 [doi]
AB  - A recognition motif is vital in determining the specificity and sensitivity of 
      the fluorescence polarization assay (FPA) for detecting chemical contaminants in 
      food. Four candidates (Gyrase, GyrBA, TopIV, and QepA) were prepared for this 
      study. The applicability of QepA was confirmed through DNA cleavage assay, 
      inhibition effects, and mechanism investigations using molecular docking, 
      compared to other counterparts. Finally, a novel FPA based on QepA and a CIP-FITC 
      tracer for the detection of fluoroquinolones (FQs) in eggs was developed. The 
      limits of detection (LODs) for eight fluoroquinolones ranged from 2.2 to 5.1 ng 
      g(-1), with enrofloxacin, danofloxacin, and difloxacin meeting the maximum 
      residue limits (MRLs). The spiked recoveries ranged from 65.8 to 103.6% with 
      coefficients of variation (CVs) of 5.4-12.8%. Therefore, a new recognition motif 
      for FQs that did not belong to conventional antibodies was identified, and 
      QepA-based FPA could be a potential tool for rapid, homogeneous, and sensitive 
      monitoring of the residue of FQs in eggs.
FAU - Jin, Qiushi
AU  - Jin Q
AD  - College of Veterinary Medicine, Hebei Agricultural University, Baoding 071000, 
      Hebei, China.
FAU - Fan, Yuhang
AU  - Fan Y
AD  - College of Veterinary Medicine, Hebei Agricultural University, Baoding 071000, 
      Hebei, China.
FAU - He, Tong
AU  - He T
AD  - College of Veterinary Medicine, Hebei Agricultural University, Baoding 071000, 
      Hebei, China.
FAU - Peng, Junling
AU  - Peng J
AD  - College of Veterinary Medicine, Hebei Agricultural University, Baoding 071000, 
      Hebei, China.
FAU - Liu, Jing
AU  - Liu J
AUID- ORCID: 0000-0002-8290-3320
AD  - College of Veterinary Medicine, Hebei Agricultural University, Baoding 071000, 
      Hebei, China.
FAU - Wang, Jianping
AU  - Wang J
AUID- ORCID: 0000-0002-2112-0218
AD  - College of Veterinary Medicine, Hebei Agricultural University, Baoding 071000, 
      Hebei, China.
LA  - eng
PT  - Journal Article
DEP - 20231129
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Fluoroquinolones)
SB  - IM
MH  - Molecular Docking Simulation
MH  - *Fluoroquinolones
MH  - *Eggs/analysis
MH  - Limit of Detection
MH  - Fluorescence Polarization
OTO - NOTNLM
OT  - QepA
OT  - egg
OT  - fluorescence polarization assay
OT  - fluoroquinolones
OT  - recognition motif
EDAT- 2023/11/29 18:42
MHDA- 2023/12/17 09:45
CRDT- 2023/11/29 17:31
PHST- 2023/12/17 09:45 [medline]
PHST- 2023/11/29 18:42 [pubmed]
PHST- 2023/11/29 17:31 [entrez]
AID - 10.1021/acs.jafc.3c03526 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2023 Dec 13;71(49):19749-19759. doi: 10.1021/acs.jafc.3c03526. 
      Epub 2023 Nov 29.