PMID- 38030141 OWN - NLM STAT- MEDLINE DCOM- 20240314 LR - 20240314 IS - 2192-2659 (Electronic) IS - 2192-2640 (Linking) VI - 13 IP - 7 DP - 2024 Mar TI - Enhancing Bone Regeneration through CDC20-Loaded ZIF-8 Nanoparticles Wrapped in Erythrocyte Membranes with Targeting Aptamer. PG - e2302725 LID - 10.1002/adhm.202302725 [doi] AB - In the context of bone regeneration, nanoparticles harboring osteogenic factors have emerged as pivotal agents for modulating the differentiation fate of stem cells. However, persistent challenges surrounding biocompatibility, loading efficiency, and precise targeting ability warrant innovative solution. In this study, a novel nanoparticle platform founded upon the zeolitic imidazolate framework-8 (ZIF-8) is introduced. This new design, CDC20@ZIF-8@eM-Apt, involves the envelopment of ZIF-8 within an erythrocyte membrane (eM) cloak, and is coupled with a targeting aptamer. ZIF-8, distinguished by its porosity, biocompatibility, and robust cargo transport capabilities, constitutes the core framework. Cell division cycle protein 20 homolog (CDC20) is illuminated as a new target in bone regeneration. The eM plays a dual role in maintaining nanoparticle stability and facilitating fusion with target cell membranes, while the aptamer orchestrates the specific recruitment of bone marrow mesenchymal stem cells (BMSCs) within bone defect sites. Significantly, CDC20@ZIF-8@eM-Apt amplifies osteogenic differentiation of BMSCs via the inhibition of NF-kappaB p65, and concurrently catalyzes bone regeneration in two bone defect models. Consequently, CDC20@ZIF-8@eM-Apt introduces a pioneering strategy for tackling bone defects and associated maladies, opening novel avenues in therapeutic intervention. CI - (c) 2023 Wiley-VCH GmbH. FAU - Du, Yangge AU - Du Y AD - State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, 430079, China. AD - Medical Research Institute, School of Medicine, Wuhan University, Wuhan, 430071, China. FAU - Deng, Tian AU - Deng T AD - State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, 430079, China. AD - Medical Research Institute, School of Medicine, Wuhan University, Wuhan, 430071, China. FAU - Cheng, Yihong AU - Cheng Y AD - State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, 430079, China. AD - Medical Research Institute, School of Medicine, Wuhan University, Wuhan, 430071, China. FAU - Zhao, Qin AU - Zhao Q AD - State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, 430079, China. AD - Medical Research Institute, School of Medicine, Wuhan University, Wuhan, 430071, China. FAU - Xia, Haibin AU - Xia H AD - State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, 430079, China. AD - Medical Research Institute, School of Medicine, Wuhan University, Wuhan, 430071, China. FAU - Ji, Yaoting AU - Ji Y AD - State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, 430079, China. AD - Medical Research Institute, School of Medicine, Wuhan University, Wuhan, 430071, China. FAU - Zhang, Yufeng AU - Zhang Y AD - State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, 430079, China. AD - Medical Research Institute, School of Medicine, Wuhan University, Wuhan, 430071, China. FAU - He, Qing AU - He Q AUID- ORCID: 0009-0009-2675-3661 AD - State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, 430079, China. AD - Medical Research Institute, School of Medicine, Wuhan University, Wuhan, 430071, China. LA - eng GR - 82072483/National Natural Science Foundation of China/ GR - 82172493/National Natural Science Foundation of China/ GR - 82101021/National Natural Science Foundation of China/ GR - 2023AFB125/Natural Science Foundation of Hubei Province/ GR - 2042023kfyq02/Fundamental Research Funds for the Central Universities/ GR - 2042023kf0139/Fundamental Research Funds for the Central Universities/ GR - 2042022kf1163/Fundamental Research Funds for the Central Universities/ GR - 2022BCA052/Key Research and Development Program of Hubei Province/ PT - Journal Article DEP - 20231210 PL - Germany TA - Adv Healthc Mater JT - Advanced healthcare materials JID - 101581613 RN - 1318-02-1 (Zeolites) SB - IM MH - Osteogenesis MH - *Zeolites MH - Erythrocyte Membrane MH - Bone Regeneration/physiology MH - *Nanoparticles OTO - NOTNLM OT - aptamer-conjugated delivery OT - cell division cycle protein 20 homolog OT - erythrocyte membrane coating OT - osteogenesis OT - zeolitic imidazolate framework-8 EDAT- 2023/11/30 00:42 MHDA- 2024/03/14 06:46 CRDT- 2023/11/29 20:03 PHST- 2023/11/15 00:00 [revised] PHST- 2023/08/18 00:00 [received] PHST- 2024/03/14 06:46 [medline] PHST- 2023/11/30 00:42 [pubmed] PHST- 2023/11/29 20:03 [entrez] AID - 10.1002/adhm.202302725 [doi] PST - ppublish SO - Adv Healthc Mater. 2024 Mar;13(7):e2302725. doi: 10.1002/adhm.202302725. Epub 2023 Dec 10.