PMID- 3803098
OWN - NLM
STAT- MEDLINE
DCOM- 19870227
LR  - 20131121
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 8
IP  - 1
DP  - 1987 Jan
TI  - Two-parameter data acquisition system for rapid slit-scan analysis of mammalian 
      chromosomes.
PG  - 83-90
AB  - A data acquisition system is described for recording two independent signals 
      simultaneously from a laser-based flow cytometer for rapid slit-scan chromosome 
      analysis. High-aperture microscope optics allow recording of fluorescence 
      distributions along the longest axis of metaphase chromosomes with a spatial 
      resolution better than 1 micron. Fluorescence and small angle forward light 
      scatter as well as dual-wavelength fluorescence signals from Indian muntjac 
      chromosomes stained with propidium iodide (PI) or acridine orange (AO) have been 
      recorded simultaneously. While maintaining the multi-user operation of the 
      computer, photomultiplier signals are digitized at a rate of 400 signals per 
      second, stored temporarily in high-speed cache memories, and transferred 
      subsequently to a minicomputer for further storage. Extensive software packages 
      for data acquisition, analysis, and display of the results are described. Data 
      acquisition is generally done in list mode, allowing complete reconstruction of 
      individual signals (profiles) at any time. The distribution of stained 
      constituents along the chromosomes can be displayed. Furthermore, histograms of 
      various parameters of the input signals may be generated.
FAU - Weier, H U
AU  - Weier HU
FAU - Eisert, W G
AU  - Eisert WG
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Algorithms
MH  - Animals
MH  - Cells, Cultured
MH  - Chromosomes/*analysis
MH  - Computers
MH  - DNA/*analysis
MH  - Deer
MH  - Flow Cytometry
MH  - Kinetics
MH  - Lasers
MH  - Male
MH  - Minicomputers
MH  - Software
EDAT- 1987/01/01 00:00
MHDA- 1987/01/01 00:01
CRDT- 1987/01/01 00:00
PHST- 1987/01/01 00:00 [pubmed]
PHST- 1987/01/01 00:01 [medline]
PHST- 1987/01/01 00:00 [entrez]
AID - 10.1002/cyto.990080112 [doi]
PST - ppublish
SO  - Cytometry. 1987 Jan;8(1):83-90. doi: 10.1002/cyto.990080112.