PMID- 38036049
OWN - NLM
STAT- MEDLINE
DCOM- 20240214
LR  - 20240214
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Linking)
VI  - 153
DP  - 2024 Apr
TI  - DNase I rescues goat sperm entrapped by neutrophil extracellular traps.
PG  - 105107
LID - S0145-305X(23)01260-0 [pii]
LID - 10.1016/j.dci.2023.105107 [doi]
AB  - Artificial insemination has been a predominant technique employed in goat 
      husbandry for breeding purposes. Subsequent to artificial insemination, sperm can 
      elicit inflammation in the reproductive tract, resulting in substantial the 
      accumulation of neutrophils. Recognized as foreign entities, sperm may become 
      entrapped within neutrophil extracellular traps (NETs) released by neutrophils, 
      thereby exploiting their properties of pathogen elimination. Deoxyribonuclease I 
      (DNase I), which is known for disintegrating NETs and causing loss of function, 
      has been utilized to ameliorate liver and brain damage resulting from NETs, as 
      well as to enhance sperm quality. This study investigated the mechanism of 
      sperm-induced NETs and further explored the impact of DNase I on NETs. Sperm 
      quality was evaluated using optical microscopy, while the structure of NETs was 
      observed through immunofluorescence staining. The formation mechanism of NETs was 
      examined using inhibitors and PicoGreen. The findings revealed that sperm induced 
      the formation of NETs, a process regulated by glycolysis, NADPH oxidase, 
      ERK(1/2), and p38 signaling pathways. The composition of NETs encompassed DNA, 
      citrullinated histone H3 (citH3), and elastase (NE). DNase I protects sperm by 
      degrading NETs, thereby concurrently preserving the integrity of plasma membrane 
      and motility of sperm. In summary, the release of sperm-induced NETs leads to its 
      damage, but this detrimental effect is counteracted by DNase I through 
      degradation of NETs. These observations provide novel insights into reproductive 
      immunity in goats.
CI  - Copyright (c) 2023 Elsevier Ltd. All rights reserved.
FAU - Wei, Zhengkai
AU  - Wei Z
AD  - College of Veterinary Medicine, Southwest University, Chongqing, 400715, PR 
      China; College of Life Sciences and Engineering, Foshan University, Foshan, 
      528225, Guangdong Province, PR China. Electronic address: wei_zhengkai@126.com.
FAU - Hong, Hongrong
AU  - Hong H
AD  - College of Life Sciences and Engineering, Foshan University, Foshan, 528225, 
      Guangdong Province, PR China.
FAU - Liu, Wei
AU  - Liu W
AD  - College of Life Sciences and Engineering, Foshan University, Foshan, 528225, 
      Guangdong Province, PR China.
FAU - Jiang, Liqiang
AU  - Jiang L
AD  - College of Life Sciences and Engineering, Foshan University, Foshan, 528225, 
      Guangdong Province, PR China.
FAU - Xu, Jingnan
AU  - Xu J
AD  - College of Life Sciences and Engineering, Foshan University, Foshan, 528225, 
      Guangdong Province, PR China.
FAU - Gao, Xinxin
AU  - Gao X
AD  - College of Life Sciences and Engineering, Foshan University, Foshan, 528225, 
      Guangdong Province, PR China.
FAU - Qian, Yuxiao
AU  - Qian Y
AD  - College of Life Sciences and Engineering, Foshan University, Foshan, 528225, 
      Guangdong Province, PR China.
FAU - Jiang, Yuqian
AU  - Jiang Y
AD  - College of Life Sciences and Engineering, Foshan University, Foshan, 528225, 
      Guangdong Province, PR China.
FAU - Jin, Zha
AU  - Jin Z
AD  - College of Life Sciences and Engineering, Foshan University, Foshan, 528225, 
      Guangdong Province, PR China.
FAU - Jin, Qinqin
AU  - Jin Q
AD  - College of Life Sciences and Engineering, Foshan University, Foshan, 528225, 
      Guangdong Province, PR China.
FAU - Chen, Meiyi
AU  - Chen M
AD  - College of Life Sciences and Engineering, Foshan University, Foshan, 528225, 
      Guangdong Province, PR China.
FAU - Yang, Zhengtao
AU  - Yang Z
AD  - College of Life Sciences and Engineering, Foshan University, Foshan, 528225, 
      Guangdong Province, PR China. Electronic address: yangzhengtao01@sina.com.
LA  - eng
PT  - Journal Article
DEP - 20231128
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - EC 3.1.21.1 (Deoxyribonuclease I)
SB  - IM
MH  - Male
MH  - Animals
MH  - *Extracellular Traps/metabolism
MH  - Goats
MH  - Semen
MH  - Neutrophils
MH  - Spermatozoa
MH  - Deoxyribonuclease I/metabolism/pharmacology
OTO - NOTNLM
OT  - Deoxyribonuclease I
OT  - Goat sperm
OT  - Neutrophil extracellular traps
OT  - Reproductive immunity
COIS- Declaration of competing interest Authors declare no conflict of interest 
      associated with this study.
EDAT- 2023/12/01 00:43
MHDA- 2024/02/10 10:50
CRDT- 2023/11/30 21:54
PHST- 2023/09/02 00:00 [received]
PHST- 2023/11/02 00:00 [revised]
PHST- 2023/11/23 00:00 [accepted]
PHST- 2024/02/10 10:50 [medline]
PHST- 2023/12/01 00:43 [pubmed]
PHST- 2023/11/30 21:54 [entrez]
AID - S0145-305X(23)01260-0 [pii]
AID - 10.1016/j.dci.2023.105107 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2024 Apr;153:105107. doi: 10.1016/j.dci.2023.105107. Epub 2023 
      Nov 28.