PMID- 38037612
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231205
IS  - 2667-0313 (Electronic)
IS  - 2667-0313 (Linking)
VI  - 3
IP  - 4
DP  - 2023 Nov
TI  - Isothiocyanate-rich moringa seed extract reduces skin inflammation in mouse ear 
      edema model.
LID - 100479 [pii]
LID - 10.1016/j.phyplu.2023.100479 [doi]
AB  - BACKGROUND: Moringa (Moringa oleifera Lam.) seed extract (MSE) and its primary 
      bioactive compound, moringa isothiocyanate-1(MIC-1), mitigate inflammation, 
      oxidative stress, diabetes, and cancer in the in vivo rodent models following 
      oral application. PURPOSE: To investigate the topical anti-inflammatory activity 
      of MSE and purified MIC-1 in a TPA-induced mouse ear edema model. STUDY DESIGN: 
      The present study elucidates the topical anti-inflammatory effects and mechanisms 
      of action of MSE, containing 38% of MIC-1 and purified MIC-1 using a mouse ear 
      edema model utilizing 12-O-tetradecanoylphorbol-13-acetate (TPA), as the 
      pro-inflammatory agent. METHODS: A time-dependent and dose-dependent response was 
      determined by pretreating CD-1 mice with various doses of MSE and MIC-1, positive 
      control, dexamethasone, or vehicle control, followed by TPA, and the subsequent 
      difference in ear thickness was measured using digital Vernier calipers. The 
      effective doses of MSE and MIC-1were then selected to evaluate the change in 
      weight of the ears using 6 mm biopsy punches and the results were confirmed by 
      microscopy. Inflammatory markers were quantified with Luminex multiplex 
      immunoassay. RESULTS: MSE and MIC-1 were effective in a dose-dependent manner in 
      a TPA-induced ear edema model, causing a reduction in ear thickness and a 48% and 
      49% decrease in ear punch weight, respectively. MSE and MIC-1 also caused a 
      reduction in the levels of cytokine and chemokines, interleukin 6 (IL-6), 
      monocyte chemoattractant protein-1 (MCP-1), and keratinocyte chemoattractant (KC) 
      in the ear tissue. MSE and MIC-1 reduced IL-6 expression by 84% and 78%, MCP1 by 
      74% and 73%, and KC by 56% and 43%, respectively. Additionally, the 
      anti-inflammatory effect of MSE and MIC-1 was confirmed by hematoxylin and eosin 
      (H&E) staining, used to assess the thickness of the ear swelling. MSE 
      significantly reduced the thickness of the ears by 20% compared to TPA. 
      CONCLUSION: These results reveal the topical anti-inflammatory properties of MSE, 
      and MIC-1 likely transmitted via the nuclear factor erythroid 2-related factor 2 
      (Nrf2) and nuclear factor-kappa B (NF-kappaB) pathways as mentioned in previous 
      studies. This work also suggests therapeutic uses of MSE and/or MIC-1 for skin 
      inflammation.
FAU - Wolff, Khea
AU  - Wolff K
AD  - Department of Plant Biology, School of Environmental and Biological Sciences, 
      Rutgers, The State University of New Jersey, New Brunswick, New Jersey, United 
      States of America.
FAU - Robinson, Keyaara
AU  - Robinson K
AD  - Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, New 
      Brunswick, New Jersey, United States of America.
FAU - Suh, Nanjoo
AU  - Suh N
AD  - Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, New 
      Brunswick, New Jersey, United States of America.
FAU - Michniak-Kohn, Bozena
AU  - Michniak-Kohn B
AD  - Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, New 
      Brunswick, New Jersey, United States of America.
FAU - Goedken, Michael
AU  - Goedken M
AD  - Rutgers Office for Research, Rutgers, The State University of New Jersey, New 
      Brunswick, New Jersey, United States of America.
FAU - Polunas, Marianne
AU  - Polunas M
AD  - Rutgers Office for Research, Rutgers, The State University of New Jersey, New 
      Brunswick, New Jersey, United States of America.
FAU - Raskin, Ilya
AU  - Raskin I
AD  - Department of Plant Biology, School of Environmental and Biological Sciences, 
      Rutgers, The State University of New Jersey, New Brunswick, New Jersey, United 
      States of America.
LA  - eng
GR  - P50 AT002776/AT/NCCIH NIH HHS/United States
PT  - Journal Article
DEP - 20230816
PL  - Netherlands
TA  - Phytomed Plus
JT  - Phytomedicine plus : international journal of phytotherapy and phytopharmacology
JID - 9918382982506676
PMC - PMC10688386
MID - NIHMS1945228
OTO - NOTNLM
OT  - Chemokines
OT  - Cytokines
OT  - Ear edema
OT  - Inflammation
OT  - Moringa isothiocyanate-1
OT  - Moringa seed extract
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/12/01 06:44
MHDA- 2023/12/01 06:45
PMCR- 2023/11/30
CRDT- 2023/12/01 03:54
PHST- 2023/12/01 06:45 [medline]
PHST- 2023/12/01 06:44 [pubmed]
PHST- 2023/12/01 03:54 [entrez]
PHST- 2023/11/30 00:00 [pmc-release]
AID - 100479 [pii]
AID - 10.1016/j.phyplu.2023.100479 [doi]
PST - ppublish
SO  - Phytomed Plus. 2023 Nov;3(4):100479. doi: 10.1016/j.phyplu.2023.100479. Epub 2023 
      Aug 16.