PMID- 38041718
OWN - NLM
STAT- MEDLINE
DCOM- 20240311
LR  - 20240311
IS  - 1678-4405 (Electronic)
IS  - 1517-8382 (Print)
IS  - 1517-8382 (Linking)
VI  - 55
IP  - 1
DP  - 2024 Mar
TI  - Natural co-infection of pigs with African swine fever virus and porcine 
      reproductive and respiratory syndrome virus in India.
PG  - 1017-1022
LID - 10.1007/s42770-023-01203-y [doi]
AB  - Porcine reproductive and respiratory syndrome (PRRS) and African swine fever 
      (ASF) are economically important diseases of pigs throughout the world. During an 
      outbreak, all age groups of animals except piglets < 1 month of age were affected 
      with symptoms of high fever, cutaneous hemorrhages, vomition with blood, 
      diarrhea, poor appetite, ataxia, and death. The outbreak was confirmed by the 
      detection of the N gene of the porcine reproductive and respiratory syndrome 
      virus (PRRSV) and the VP72 gene of the African swine fever virus (ASFV) by PCR in 
      representative blood samples from affected pigs followed by Sanger sequencing. 
      Mixed infection was also confirmed by simultaneous detection of both the viruses 
      using multiplex PCR. Phylogenetic analysis of both the viruses revealed that the 
      outbreak was related to ASFV and PRRSV strains from China which were also closely 
      related to the PRRSV and ASFV strains from the recent outbreak from India. The 
      study confirmed the involvement of genotype II of ASFV and genotype 2 of PRRSV in 
      the present outbreak. Interestingly, PRRSV associated with the present outbreak 
      was characterized as a highly pathogenic PRRSV. Therefore, the present study 
      indicates the possibility of future waves or further outbreaks of these diseases 
      (PRRS and ASF) in this region. This is the first report of ASFV and PRRSV 
      co-infection in pigs from India.
CI  - (c) 2023. The Author(s) under exclusive licence to Sociedade Brasileira de 
      Microbiologia.
FAU - Rajkhowa, Swaraj
AU  - Rajkhowa S
AUID- ORCID: 0000-0001-6296-1286
AD  - ICAR-National Research Centre on Pig, Rani, Guwahati, Assam, 781131, India. 
      swaraj.rajkhowa@gmail.com.
FAU - Sonowal, Joyshikh
AU  - Sonowal J
AD  - ICAR-National Research Centre on Pig, Rani, Guwahati, Assam, 781131, India.
FAU - Pegu, Seema Rani
AU  - Pegu SR
AD  - ICAR-National Research Centre on Pig, Rani, Guwahati, Assam, 781131, India.
FAU - Sanger, Gyanendra Singh
AU  - Sanger GS
AD  - ICAR-National Research Centre on Pig, Rani, Guwahati, Assam, 781131, India.
FAU - Deb, Rajib
AU  - Deb R
AD  - ICAR-National Research Centre on Pig, Rani, Guwahati, Assam, 781131, India.
FAU - Das, Pranab Jyoti
AU  - Das PJ
AD  - ICAR-National Research Centre on Pig, Rani, Guwahati, Assam, 781131, India.
FAU - Doley, Juwar
AU  - Doley J
AD  - ICAR-National Research Centre on Pig, Rani, Guwahati, Assam, 781131, India.
FAU - Paul, Souvik
AU  - Paul S
AD  - ICAR-National Research Centre on Pig, Rani, Guwahati, Assam, 781131, India.
FAU - Gupta, Vivek Kumar
AU  - Gupta VK
AD  - ICAR-National Research Centre on Pig, Rani, Guwahati, Assam, 781131, India.
LA  - eng
PT  - Journal Article
DEP - 20231202
PL  - Brazil
TA  - Braz J Microbiol
JT  - Brazilian journal of microbiology : [publication of the Brazilian Society for 
      Microbiology]
JID - 101095924
SB  - IM
MH  - Swine
MH  - Animals
MH  - *Porcine respiratory and reproductive syndrome virus/genetics
MH  - *African Swine Fever Virus/genetics
MH  - *Porcine Reproductive and Respiratory Syndrome/epidemiology
MH  - *African Swine Fever/epidemiology
MH  - *Coinfection/epidemiology/veterinary
MH  - Phylogeny
PMC - PMC10920511
OTO - NOTNLM
OT  - ASFV
OT  - Co-infection
OT  - Molecular detection
OT  - PRRSV
OT  - Pigs
COIS- The authors declare no competing interests.
EDAT- 2023/12/02 12:42
MHDA- 2024/03/11 06:42
PMCR- 2024/12/02
CRDT- 2023/12/02 11:04
PHST- 2023/05/13 00:00 [received]
PHST- 2023/11/28 00:00 [accepted]
PHST- 2024/12/02 00:00 [pmc-release]
PHST- 2024/03/11 06:42 [medline]
PHST- 2023/12/02 12:42 [pubmed]
PHST- 2023/12/02 11:04 [entrez]
AID - 10.1007/s42770-023-01203-y [pii]
AID - 1203 [pii]
AID - 10.1007/s42770-023-01203-y [doi]
PST - ppublish
SO  - Braz J Microbiol. 2024 Mar;55(1):1017-1022. doi: 10.1007/s42770-023-01203-y. Epub 
      2023 Dec 2.