PMID- 38043312
OWN - NLM
STAT- MEDLINE
DCOM- 20240115
LR  - 20240115
IS  - 1095-8630 (Electronic)
IS  - 0301-4797 (Linking)
VI  - 351
DP  - 2024 Feb
TI  - Enhancing the sustainable immobilization of laccase by amino-functionalized 
      PMMA-reinforced graphene nanomaterial.
PG  - 119503
LID - S0301-4797(23)02291-0 [pii]
LID - 10.1016/j.jenvman.2023.119503 [doi]
AB  - Human health and the environment are negatively affected by endocrine-disrupting 
      chemicals (EDCs), such as bisphenol A. Therefore, developing appropriate 
      remediation methods is essential for efficiently removing phenolic compounds from 
      aqueous solutions. Enzymatic biodegradation is a potential biotechnological 
      approach for responsibly addressing water pollution. With its high catalytic 
      efficiency and few by-products, laccase is an eco-friendly biocatalyst with 
      significant promise for biodegradation. Herein, two novel supporting materials 
      (NH(2)-PMMA and NH(2)-PMMA-Gr) were fabricated via the functionalization of 
      poly(methylmethacrylate) (PMMA) polymer using ethylenediamine and reinforced with 
      graphene followed by glutaraldehyde activation. NH(2)-PMMA and NH(2)-PMMA-Gr were 
      utilized for laccase immobilization with an immobilization yield (IY%) of 78.3% 
      and 82.5% and an activity yield (AY%) of 81.2% and 85.9%, respectively. Scanning 
      electron microscope (SEM) and Fourier-transform infrared (FTIR) were used to 
      study the characteristics of fabricated material supports. NH(2)-PMMA-Gr@laccase 
      exhibited an optimal pH profile from 4.5 to 5.0, while NH(2)-PMMA@laccase 
      exhibited optimum pH at 5.0 compared to a value of 4.0 for free form. A wider 
      temperature ranges of 40-50  degrees C was noted for both immobilized laccases compared 
      to a value of 40  degrees C for the free form. Additionally, it was reported that 
      immobilized laccase outperformed free laccase in terms of substrate affinity and 
      storage stability. NH(2)-PMMA@laccase and NH(2)-PMMA-Gr@laccase improved 
      stability by up to 3.9 and 4.6-fold when stored for 30 days at 4  degrees C and preserved 
      up to 80.5% and 86.7% of relative activity after ten cycles of reuse. Finally, 
      the degradation of BPA was achieved using NH(2)-PMMA@laccase and 
      NH(2)-PMMA-Gr@laccase. After five cycles, NH(2)-PMMA@laccase and 
      NH(2)-PMMA-Gr@laccase showed that the residual degradation of BPA was 77% and 
      84.5% using 50 mum of BPA. This study introduces a novel, high-performance 
      material for organic pollution remediation in wastewater that would inspire 
      further progress.
CI  - Copyright (c) 2023 Elsevier Ltd. All rights reserved.
FAU - Zayed, Mohie E M
AU  - Zayed MEM
AD  - Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, 
      21589, Saudi Arabia.
FAU - Obaid, Abdullah Y
AU  - Obaid AY
AD  - Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, 
      21589, Saudi Arabia.
FAU - Almulaiky, Yaaser Q
AU  - Almulaiky YQ
AD  - Department of Chemistry, College of Science and Arts at Khulis, University of 
      Jeddah, Jeddah, 21921, Saudi Arabia.
FAU - El-Shishtawy, Reda M
AU  - El-Shishtawy RM
AD  - Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, 
      21589, Saudi Arabia. Electronic address: elshishtawy@hotmail.com.
LA  - eng
PT  - Journal Article
DEP - 20231203
PL  - England
TA  - J Environ Manage
JT  - Journal of environmental management
JID - 0401664
RN  - 0 (Enzymes, Immobilized)
RN  - EC 1.10.3.2 (Laccase)
RN  - 7782-42-5 (Graphite)
RN  - 9011-14-7 (Polymethyl Methacrylate)
SB  - IM
MH  - Humans
MH  - Enzymes, Immobilized/chemistry/metabolism
MH  - Laccase/chemistry/metabolism
MH  - *Graphite
MH  - Polymethyl Methacrylate
MH  - Hydrogen-Ion Concentration
MH  - *Nanostructures
OTO - NOTNLM
OT  - Bisphenol A
OT  - Degradation
OT  - Graphene nanomaterial
OT  - Immobilization
OT  - Laccase
OT  - PMMA polymer
OT  - Reusability
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/12/04 00:42
MHDA- 2024/01/15 12:43
CRDT- 2023/12/03 18:08
PHST- 2023/03/22 00:00 [received]
PHST- 2023/10/09 00:00 [revised]
PHST- 2023/10/26 00:00 [accepted]
PHST- 2024/01/15 12:43 [medline]
PHST- 2023/12/04 00:42 [pubmed]
PHST- 2023/12/03 18:08 [entrez]
AID - S0301-4797(23)02291-0 [pii]
AID - 10.1016/j.jenvman.2023.119503 [doi]
PST - ppublish
SO  - J Environ Manage. 2024 Feb;351:119503. doi: 10.1016/j.jenvman.2023.119503. Epub 
      2023 Dec 3.