PMID- 38043703
OWN - NLM
STAT- MEDLINE
DCOM- 20240129
LR  - 20240206
IS  - 1535-9484 (Electronic)
IS  - 1535-9476 (Print)
IS  - 1535-9476 (Linking)
VI  - 23
IP  - 1
DP  - 2024 Jan
TI  - IMBAS-MS Discovers Organ-Specific HLA Peptide Patterns in Plasma.
PG  - 100689
LID - S1535-9476(23)00200-1 [pii]
LID - 10.1016/j.mcpro.2023.100689 [doi]
LID - 100689
AB  - Distinction of non-self from self is the major task of the immune system. 
      Immunopeptidomics studies the peptide repertoire presented by the human leukocyte 
      antigen (HLA) protein, usually on tissues. However, HLA peptides are also bound 
      to plasma soluble HLA (sHLA), but little is known about their origin and 
      potential for biomarker discovery in this readily available biofluid. Currently, 
      immunopeptidomics is hampered by complex workflows and limited sensitivity, 
      typically requiring several mL of plasma. Here, we take advantage of recent 
      improvements in the throughput and sensitivity of mass spectrometry (MS)-based 
      proteomics to develop a highly sensitive, automated, and economical workflow for 
      HLA peptide analysis, termed Immunopeptidomics by Biotinylated Antibodies and 
      Streptavidin (IMBAS). IMBAS-MS quantifies more than 5000 HLA class I peptides 
      from only 200 mul of plasma, in just 30 min. Our technology revealed that the 
      plasma immunopeptidome of healthy donors is remarkably stable throughout the year 
      and strongly correlated between individuals with overlapping HLA types. 
      Immunopeptides originating from diverse tissues, including the brain, are 
      proportionately represented. We conclude that sHLAs are a promising avenue for 
      immunology and potentially for precision oncology.
CI  - Copyright (c) 2023 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Wahle, Maria
AU  - Wahle M
AD  - Department Proteomics and Signal Transduction, Max Planck Institute of 
      Biochemistry, Martinsried, Germany.
FAU - Thielert, Marvin
AU  - Thielert M
AD  - Department Proteomics and Signal Transduction, Max Planck Institute of 
      Biochemistry, Martinsried, Germany.
FAU - Zwiebel, Maximilian
AU  - Zwiebel M
AD  - Department Proteomics and Signal Transduction, Max Planck Institute of 
      Biochemistry, Martinsried, Germany.
FAU - Skowronek, Patricia
AU  - Skowronek P
AD  - Department Proteomics and Signal Transduction, Max Planck Institute of 
      Biochemistry, Martinsried, Germany.
FAU - Zeng, Wen-Feng
AU  - Zeng WF
AD  - Department Proteomics and Signal Transduction, Max Planck Institute of 
      Biochemistry, Martinsried, Germany.
FAU - Mann, Matthias
AU  - Mann M
AD  - Department Proteomics and Signal Transduction, Max Planck Institute of 
      Biochemistry, Martinsried, Germany. Electronic address: mmann@biochem.mpg.de.
LA  - eng
PT  - Journal Article
DEP - 20231201
PL  - United States
TA  - Mol Cell Proteomics
JT  - Molecular & cellular proteomics : MCP
JID - 101125647
RN  - 9013-20-1 (Streptavidin)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Peptides)
RN  - 0 (Antibodies)
SB  - IM
MH  - Humans
MH  - Streptavidin
MH  - *Neoplasms
MH  - Precision Medicine
MH  - Histocompatibility Antigens Class I/metabolism
MH  - HLA Antigens
MH  - Histocompatibility Antigens Class II
MH  - Peptides/metabolism
MH  - Mass Spectrometry
MH  - Antibodies
PMC - PMC10765297
OTO - NOTNLM
OT  - HLA
OT  - IMBAS-MS
OT  - immunopeptidomics
OT  - plasma immunopeptidomics
OT  - sHLA
COIS- Conflict of interest M. M. is an indirect investor in Evosep Biosystems. All 
      other authors declare that they have no conflicts of interest with the contents 
      of this article.
EDAT- 2023/12/04 00:42
MHDA- 2024/01/29 06:43
PMCR- 2023/12/01
CRDT- 2023/12/03 19:27
PHST- 2023/08/22 00:00 [received]
PHST- 2023/11/24 00:00 [revised]
PHST- 2023/11/28 00:00 [accepted]
PHST- 2024/01/29 06:43 [medline]
PHST- 2023/12/04 00:42 [pubmed]
PHST- 2023/12/03 19:27 [entrez]
PHST- 2023/12/01 00:00 [pmc-release]
AID - S1535-9476(23)00200-1 [pii]
AID - 100689 [pii]
AID - 10.1016/j.mcpro.2023.100689 [doi]
PST - ppublish
SO  - Mol Cell Proteomics. 2024 Jan;23(1):100689. doi: 10.1016/j.mcpro.2023.100689. 
      Epub 2023 Dec 1.