PMID- 38045758
OWN - NLM
STAT- MEDLINE
DCOM- 20231205
LR  - 20240221
IS  - 2235-2988 (Electronic)
IS  - 2235-2988 (Linking)
VI  - 13
DP  - 2023
TI  - Proteomic analysis reveals changes in the proteome of human THP-1 macrophages 
      infected with Paracoccidioides brasiliensis.
PG  - 1275954
LID - 10.3389/fcimb.2023.1275954 [doi]
LID - 1275954
AB  - Paracoccidioides spp. is the etiologic agent of Paracoccidioidomycosis (PCM), a 
      systemic disease with wide distribution in Latin America. Macrophages are very 
      important cells during the response to infection by P. brasiliensis. In this 
      study, we performed a proteomic analysis to evaluate the consequences of P. 
      brasiliensis yeast cells on the human THP-1 macrophage proteome. We have 
      identified 443 and 2247 upregulated or downregulated proteins, respectively, in 
      macrophages co-cultured with yeast cells of P. brasiliensis in comparison to 
      control macrophages unexposed to the fungus. Proteomic analysis revealed that 
      interaction with P. brasiliensis caused metabolic changes in macrophages that 
      drastically affected energy production pathways. In addition, these macrophages 
      presented regulated many factors related to epigenetic modifications and gene 
      transcription as well as a decrease of many proteins associated to the immune 
      system activity. This is the first human macrophage proteome derived from 
      interactions with P. brasiliensis, which contributes to elucidating the changes 
      that occur during the host response to this fungus. Furthermore, it highlights 
      proteins that may be targets for the development of new therapeutic approaches to 
      PCM.
CI  - Copyright (c) 2023 de Figueiredo, Moraes, Bailao, Rocha, Silva, Ribeiro-Dias and 
      Soares.
FAU - de Figueiredo, Ana Marina Barroso
AU  - de Figueiredo AMB
AD  - Laboratorio de Imunidade Natural (LIN), Instituto de Patologia Tropical e Saude 
      Publica, Universidade Federal de Goias, Goiania, Goias, Brazil.
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania, Goias, Brazil.
FAU - Moraes, Dayane
AU  - Moraes D
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania, Goias, Brazil.
FAU - Bailao, Alexandre Melo
AU  - Bailao AM
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania, Goias, Brazil.
FAU - Rocha, Olivia Basso
AU  - Rocha OB
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania, Goias, Brazil.
FAU - Silva, Lana Ohara Souza
AU  - Silva LOS
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania, Goias, Brazil.
FAU - Ribeiro-Dias, Fatima
AU  - Ribeiro-Dias F
AD  - Laboratorio de Imunidade Natural (LIN), Instituto de Patologia Tropical e Saude 
      Publica, Universidade Federal de Goias, Goiania, Goias, Brazil.
FAU - Soares, Celia Maria de Almeida
AU  - Soares CMA
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania, Goias, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231116
PL  - Switzerland
TA  - Front Cell Infect Microbiol
JT  - Frontiers in cellular and infection microbiology
JID - 101585359
RN  - 0 (Proteome)
SB  - IM
MH  - Humans
MH  - *Paracoccidioides
MH  - Proteome/metabolism
MH  - Saccharomyces cerevisiae
MH  - Proteomics
MH  - Macrophages/microbiology
PMC - PMC10693345
OTO - NOTNLM
OT  - Paracoccidioides brasiliensis
OT  - immunometabolism
OT  - macrophages
OT  - metabolic reprogramming
OT  - proteomic analysis
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/12/04 06:42
MHDA- 2023/12/05 17:44
PMCR- 2023/01/01
CRDT- 2023/12/04 04:43
PHST- 2023/08/10 00:00 [received]
PHST- 2023/10/24 00:00 [accepted]
PHST- 2023/12/05 17:44 [medline]
PHST- 2023/12/04 06:42 [pubmed]
PHST- 2023/12/04 04:43 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 10.3389/fcimb.2023.1275954 [doi]
PST - epublish
SO  - Front Cell Infect Microbiol. 2023 Nov 16;13:1275954. doi: 
      10.3389/fcimb.2023.1275954. eCollection 2023.