PMID- 38047016
OWN - NLM
STAT- MEDLINE
DCOM- 20231205
LR  - 20231216
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 11
DP  - 2023
TI  - Excessive aggregation of fine particles may play a crucial role in adolescent 
      spontaneous pneumothorax pathogenesis.
PG  - e16484
LID - 10.7717/peerj.16484 [doi]
LID - e16484
AB  - BACKGROUND: The pathogenesis of primary spontaneous pneumothorax (PSP) is 
      unclear. Fine particles aggregated in the lung can be phagocytosed by alveolar 
      macrophages (AMs) to induce an inflammatory reaction and damage local pulmonary 
      tissue, which could be a mechanism of PSP. This project aimed to explore the 
      pathological association between fine particulate matter and PSP. METHODS: Thirty 
      pulmonary bullae tissues were obtained from surgery of PSP patients (B group). 
      The adjacent normal tissues of the lungs were defined as the control S group. 
      Another 30 normal lung tissues with nonpneumothorax disease (NPD) were applied as 
      the control N group. Hematoxylin and eosin (H & E), Wright-Giemsa (W-G), Victoria 
      blue, and immunohistochemical (IHC) staining experiments were performed to 
      measure the levels of fine particulate matter, alveolar macrophages (AMs), 
      pulmonary elastic fibers, monocyte chemoattractant protein-1 (MCP-1), and matrix 
      metalloproteinase-9 (MMP-9) in the lung tissues. The serum levels of MCP-1 and 
      MMP-9 were prospectively analyzed as well. RESULTS: Histopathological 
      examinations revealed obvious deposition of fine particulate matter and 
      inflammatory reactions (proliferation of AMs) in the B group, compared with those 
      in the S group and the N group. These alterations were significantly associated 
      with PSP. The numbers of AMs and pulmonary elastic fibers, the positive area of 
      the H-score, as well as the concentrations of MCP-1 and MMP-9 in the lungs of the 
      experimental group were obviously raised compared with the controls (P < 0.05). 
      CONCLUSIONS: Fine particulate matter aggregation, inflammation (macrophage 
      hyperplasia), and overexpression of MCP-1 and MMP-9 may contribute to the 
      pathogenesis of PSP. The overaccumulation of fine particulate matter may play a 
      crucial part in the occurrence of adolescent and young adult PSP. TRIAL 
      REGISTRATION: This project was enrolled on the Chinese Clinical Trial Registry: 
      ChiCTR2100051460.
CI  - (c)2023 Wang et al.
FAU - Wang, Sibo
AU  - Wang S
AD  - Department of Cardiothoracic Surgery, The Southern Yunnan Central Hospital of 
      Yunnan/The First People's Hospital of Honghe Prefecture, Gejiu, Yunnan, China.
FAU - Li, Jun
AU  - Li J
AD  - Department of Cardiothoracic Surgery, The Southern Yunnan Central Hospital of 
      Yunnan/The First People's Hospital of Honghe Prefecture, Gejiu, Yunnan, China.
FAU - Qian, Mengjiao
AU  - Qian M
AD  - Department of Cardiothoracic Surgery, The Southern Yunnan Central Hospital of 
      Yunnan/The First People's Hospital of Honghe Prefecture, Gejiu, Yunnan, China.
FAU - Wang, Jing
AU  - Wang J
AD  - Department of Cardiothoracic Surgery, The Southern Yunnan Central Hospital of 
      Yunnan/The First People's Hospital of Honghe Prefecture, Gejiu, Yunnan, China.
FAU - Tan, Yongxing
AU  - Tan Y
AD  - Department of Pathology, The Southern Yunnan Central Hospital of Yunnan/The First 
      People's Hospital of Honghe Prefecture, Gejiu, Yunnan, China.
FAU - Ou, Haibo
AU  - Ou H
AD  - Department of Cardiothoracic Surgery, The Southern Yunnan Central Hospital of 
      Yunnan/The First People's Hospital of Honghe Prefecture, Gejiu, Yunnan, China.
FAU - Wang, Zhongyin
AU  - Wang Z
AD  - Department of Cardiothoracic Surgery, The Southern Yunnan Central Hospital of 
      Yunnan/The First People's Hospital of Honghe Prefecture, Gejiu, Yunnan, China.
FAU - Chen, Xiao
AU  - Chen X
AD  - Department of Cardiothoracic Surgery, The Southern Yunnan Central Hospital of 
      Yunnan/The First People's Hospital of Honghe Prefecture, Gejiu, Yunnan, China.
FAU - Tu, Yunjiao
AU  - Tu Y
AD  - Department of Pathology, The Southern Yunnan Central Hospital of Yunnan/The First 
      People's Hospital of Honghe Prefecture, Gejiu, Yunnan, China.
FAU - Xu, Kai
AU  - Xu K
AD  - Department of Clinical Laboratory, The Southern Yunnan Central Hospital of 
      Yunnan/The First People's Hospital of Honghe Prefecture, Gejiu, Yunnan, China.
LA  - eng
PT  - Journal Article
DEP - 20231129
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - 0 (Particulate Matter)
SB  - IM
MH  - Young Adult
MH  - Humans
MH  - Adolescent
MH  - *Pneumothorax/pathology
MH  - Matrix Metalloproteinase 9
MH  - Lung/pathology
MH  - *Lung Diseases/pathology
MH  - Particulate Matter/adverse effects
PMC - PMC10693242
OTO - NOTNLM
OT  - Alveolar macrophage
OT  - Fine particulate matter
OT  - Matrix metalloproteinase-9
OT  - Monocyte chemoattractant protein-1
OT  - Primary spontaneous pneumothorax
OT  - Pulmonary elastic fiber
COIS- The authors declare there are no competing interests.
EDAT- 2023/12/04 06:41
MHDA- 2023/12/05 17:45
PMCR- 2023/11/29
CRDT- 2023/12/04 05:16
PHST- 2023/07/18 00:00 [received]
PHST- 2023/10/27 00:00 [accepted]
PHST- 2023/12/05 17:45 [medline]
PHST- 2023/12/04 06:41 [pubmed]
PHST- 2023/12/04 05:16 [entrez]
PHST- 2023/11/29 00:00 [pmc-release]
AID - 16484 [pii]
AID - 10.7717/peerj.16484 [doi]
PST - epublish
SO  - PeerJ. 2023 Nov 29;11:e16484. doi: 10.7717/peerj.16484. eCollection 2023.