PMID- 38049081
OWN - NLM
STAT- MEDLINE
DCOM- 20240110
LR  - 20240119
IS  - 1090-2422 (Electronic)
IS  - 0014-4827 (Linking)
VI  - 434
IP  - 2
DP  - 2024 Jan 15
TI  - Dual-specificity phosphatase 5-mediated fatty acid oxidation promotes 
      Mycobacterium bovis BCG -induced inflammatory responses.
PG  - 113869
LID - S0014-4827(23)00420-2 [pii]
LID - 10.1016/j.yexcr.2023.113869 [doi]
AB  - Mycobacterium tuberculosis (Mtb) reprograms FAs metabolism of macrophages during 
      infection and affects inflammatory reaction eventually, however, the mechanism 
      remains poorly understood. Here we show that Mycobacterium bovis (BCG) induces 
      DUSP5 expression through TLR2-MAPKs signaling pathway and promotes fatty acid 
      oxidation (FAO). Silencing DUSP5 by adeno-associated virus vector (AAV) 
      ameliorates lung injury and DUSP5 knockdown reduces the expression of IL-1beta, IL-6 
      and inactivated NF-kappaB signaling in BCG-infected macrophages. Of note, DUSP5 
      specific siRNA increases the content of free fatty acids (FFAs) and triglyceride 
      (TG), but represses the expression of FAO associated enzymes such as CPT1A and 
      PPARalpha, suggesting DUSP5 mediated FAO during BCG infection. Moreover, Inhibiting 
      FAO by pharmacological manner suppresses IL-1beta, IL-6, TNF-alpha expression and 
      relieves lung damage. Taken together, our data indicates DUSP5 mediates FAO 
      reprogramming and promotes inflammatory response to BCG infection.
CI  - Copyright (c) 2023 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Luo, Jia
AU  - Luo J
AD  - Institute of Medical Sciences, General Hospital of Ningxia Medical University, 
      Yinchuan, Ningxia, 750004, China; General Hospital of Ningxia Medical University, 
      Yinchuan, Ningxia, 750004, China.
FAU - Tian, Zengjian
AU  - Tian Z
AD  - School of Clinical Medicine, Ningxia Medical University, Yinchuan, Ningxia, 
      750004, China.
FAU - Song, Fuyang
AU  - Song F
AD  - College of Life Science, Ningxia University, Yinchuan, Ningxia, 750021, China.
FAU - Ren, Chao
AU  - Ren C
AD  - General Hospital of Ningxia Medical University, Yinchuan, Ningxia, 750004, China.
FAU - Liu, Wenmiao
AU  - Liu W
AD  - General Hospital of Ningxia Medical University, Yinchuan, Ningxia, 750004, China; 
      The Center of Laboratory Medicine, General Hospital of Ningxia Medical 
      University, Yinchuan, Ningxia, 750004, China. Electronic address: 
      liuwenmiao@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231202
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Interleukin-6)
RN  - EC 3.1.3.48 (Dual-Specificity Phosphatases)
RN  - 0 (Fatty Acids)
SB  - IM
MH  - *Mycobacterium bovis
MH  - Interleukin-6/genetics/metabolism
MH  - Signal Transduction
MH  - Dual-Specificity Phosphatases/genetics
MH  - Fatty Acids
OTO - NOTNLM
OT  - BCG
OT  - Dual specificity phosphatase 5
OT  - Fatty acid oxidation
OT  - Inflammation
OT  - Macrophage
COIS- Declaration of competing interest This work has not been submitted to any other 
      journals and all the authors have contributed to the paper and have reviewed the 
      manuscript and agree with its contents.
EDAT- 2023/12/05 00:41
MHDA- 2024/01/10 06:42
CRDT- 2023/12/04 19:27
PHST- 2023/09/12 00:00 [received]
PHST- 2023/11/24 00:00 [revised]
PHST- 2023/11/28 00:00 [accepted]
PHST- 2024/01/10 06:42 [medline]
PHST- 2023/12/05 00:41 [pubmed]
PHST- 2023/12/04 19:27 [entrez]
AID - S0014-4827(23)00420-2 [pii]
AID - 10.1016/j.yexcr.2023.113869 [doi]
PST - ppublish
SO  - Exp Cell Res. 2024 Jan 15;434(2):113869. doi: 10.1016/j.yexcr.2023.113869. Epub 
      2023 Dec 2.