PMID- 38050447
OWN - NLM
STAT- MEDLINE
DCOM- 20240118
LR  - 20240118
IS  - 1099-0461 (Electronic)
IS  - 1095-6670 (Linking)
VI  - 38
IP  - 1
DP  - 2024 Jan
TI  - Nanocurcumin attenuates pyroptosis and inflammation through inhibiting 
      NF-kappaB/GSDMD signal in high altitude-associated acute liver injury.
PG  - e23606
LID - 10.1002/jbt.23606 [doi]
AB  - Exposure to a hypobaric hypoxic environment at high altitudes can lead to liver 
      injury, and mounting evidence indicates that pyroptosis and inflammation play 
      important roles in liver injury. Curcumin (Cur) can inhibit pyroptosis and 
      inflammation. Therefore, our purpose here was to clarify the mechanism underlying 
      the protective effect of nanocurcumin (Ncur) and Cur in a rat model of high 
      altitude-associated acute liver injury. Eighty healthy rats were selected and 
      exposed to different altitudes (6000 or 7000 m) for 0, 24, 48, or 72 h. Fifty 
      normal healthy rats were divided into normal control, high-altitude control, 
      salidroside (40 mg/kg [Sal-40]), Cur (200 mg/kg [Cur-200]), and Ncur (25 mg/kg 
      [Ncur-25]) groups and exposed to a high-altitude hypobaric hypoxic environment 
      (48 h, 7000 m). Serum-liver enzyme activities (alanine transaminase, aspartate 
      transaminase, and lactate dehydrogenase were detected and histopathology of liver 
      injury was evaluated by hematoxylin and eosin staining, and inflammatory factors 
      were detected in liver tissues by enzyme-linked immunosorbent assays. 
      Pyroptosis-associated proteins (gasdermin D, gasdermin D N-terminal [GSDMD-N], 
      pro-Caspase-1, and cleaved-Caspase-1 [cleaved-Casp1]) and inflammation-associated 
      proteins (nuclear factor-kappaB [NF-kappaB], phospho-NF-kappaB [P-NF-kappaB], and high-mobility 
      group protein B1 [HMGB1]) levels were analyzed by immunoblotting. Ncur and Cur 
      inhibited increased serum-liver enzyme activities, alleviated liver injury in 
      rats caused by high-altitude hypobaric hypoxic exposure, and downregulated 
      inflammatory factors, including tumor necrosis factor-alpha, interleukin (IL)-1beta, 
      IL-6, and IL-18, in rat liver tissues. The level of P-NF-kappaB, GSDMD-N, 
      cleaved-Casp1, and HMGB1 in rat liver tissues increased significantly after 
      high-altitude exposure. Ncur and Cur downregulated P-NF-kappaB, GSDMD-N, 
      cleaved-Casp-1, and HMGB1. Ncur and Cur may inhibit inflammatory responses and 
      pyroptosis in a rat model of high altitude-associated acute liver injury.
CI  - (c) 2023 The Authors. Journal of Biochemical and Molecular Toxicology published by 
      Wiley Periodicals LLC.
FAU - Yang, Xinyue
AU  - Yang X
AUID- ORCID: 0000-0001-8230-1322
AD  - Key Laboratory of Special Environmental Medicine of Xinjiang, General Hospital of 
      Xinjang Military Command, Urumqi, China.
AD  - Graduate School, Xinjiang Medical University, Urumqi, China.
FAU - Dong, Xiang
AU  - Dong X
AUID- ORCID: 0000-0001-8690-7735
AD  - Graduate School, Xinjiang Medical University, Urumqi, China.
FAU - Li, Jiajia
AU  - Li J
AUID- ORCID: 0000-0003-3406-9703
AD  - Graduate School, Xinjiang Medical University, Urumqi, China.
FAU - Zheng, Aiping
AU  - Zheng A
AD  - Institute of Pharmacology and Toxicology, Academy of Military Medicine, Beijing, 
      China.
FAU - Shi, Wenhui
AU  - Shi W
AUID- ORCID: 0000-0002-4338-4046
AD  - Graduate School, Xinjiang Medical University, Urumqi, China.
FAU - Shen, Caifu
AU  - Shen C
AUID- ORCID: 0000-0002-4656-5344
AD  - Graduate School, Xinjiang Medical University, Urumqi, China.
FAU - Liu, Jiangwei
AU  - Liu J
AUID- ORCID: 0000-0002-7652-9379
AD  - Graduate School, Xinjiang Medical University, Urumqi, China.
LA  - eng
GR  - 2019D04022/Xinjiang Uygur Autonomous Region Department of Education/
PT  - Journal Article
DEP - 20231204
PL  - United States
TA  - J Biochem Mol Toxicol
JT  - Journal of biochemical and molecular toxicology
JID - 9717231
RN  - 0 (NF-kappa B)
RN  - 0 (HMGB1 Protein)
RN  - 0 (Gasdermins)
RN  - EC 3.4.22.36 (Caspase 1)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
SB  - IM
MH  - Rats
MH  - Animals
MH  - NF-kappa B/metabolism
MH  - Pyroptosis
MH  - *HMGB1 Protein/metabolism
MH  - Altitude
MH  - Gasdermins
MH  - Inflammation/drug therapy/metabolism
MH  - *Liver Diseases/metabolism
MH  - Caspase 1/metabolism
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
OTO - NOTNLM
OT  - acute liver injury
OT  - hypobaric hypoxia
OT  - inflammation
OT  - nanocurcumin
OT  - pyroptosis
EDAT- 2023/12/05 12:42
MHDA- 2024/01/18 06:42
CRDT- 2023/12/05 03:44
PHST- 2023/07/12 00:00 [revised]
PHST- 2022/05/07 00:00 [received]
PHST- 2023/11/21 00:00 [accepted]
PHST- 2024/01/18 06:42 [medline]
PHST- 2023/12/05 12:42 [pubmed]
PHST- 2023/12/05 03:44 [entrez]
AID - 10.1002/jbt.23606 [doi]
PST - ppublish
SO  - J Biochem Mol Toxicol. 2024 Jan;38(1):e23606. doi: 10.1002/jbt.23606. Epub 2023 
      Dec 4.