PMID- 38053543
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231207
IS  - 2699-9404 (Electronic)
IS  - 2699-9404 (Linking)
VI  - 10
IP  - 4
DP  - 2023 Dec
TI  - Study on Screening Core Biomarkers of Noise and Drug-Induced Hearing Loss Based 
      on Transcriptomics.
PG  - 357-369
LID - 10.1055/s-0043-1777069 [doi]
AB  - Background  Noise and drug-induced hearing loss (HL) is becoming more and more 
      serious, but the integration and analysis based on transcriptomics and proteomics 
      are lacking. On the one hand, this study aims to integrate existing public 
      transcriptomic data on noise and gentamicin-induced HL. On the other hand, the 
      study aims to establish the gentamicin and noise-induced HL model of guinea pigs, 
      then to perform the transcriptomic and proteomic analyses. Through comprehensive 
      analysis of the above data, we aim to screen, predict, and preliminarily verify 
      biomarkers closely related to HL. Material and Methods  We screened the Gene 
      Expression Omnibus database to obtain transcriptome data expression profiles of 
      HL caused by noise and gentamicin, then constructed the guinea pig HL model and 
      perform the transcriptomic and proteomic analyses. Differential expression and 
      enrichment analysis were performed on public and self-sequenced data, and common 
      differentially expressed genes (DEGs) and signaling pathways were obtained. 
      Finally, we used proteomic data to screen for common differential proteins and 
      validate common differential expression genes for HL. Results  By integrating the 
      public data set with self-constructed model data set, we eventually obtained two 
      core biomarkers of HL, which were RSAD2 and matrix metalloproteinase-3 (MMP3). 
      Their main function is to regulate the development of sense organ in the inner 
      ear and they are mainly involved in mitogen-activated protein kinase and 
      phosphoinositol-3 kinase/protein kinase B signaling pathways. Finally, by 
      integrating the proteomic data of the self-constructed model, we also found 
      differential expression of MMP3 protein. This also preliminarily and partially 
      verified the above-mentioned core biomarkers. Conclusion and Significance  In 
      this study, public database and transcriptomic data of self-constructed model 
      were integrated, and we screened out two core genes and various signal pathways 
      of HL through differential analysis, enrichment analysis, and other analysis 
      methods. Then, we preliminarily validated the MMP3 by proteomic analysis of 
      self-constructed model. This study pointed out the direction for further 
      laboratory verification of key biomarkers of HL, which is of great significance 
      for revealing the core pathogenic mechanism of HL.
CI  - The Author(s). This is an open access article published by Thieme under the terms 
      of the Creative Commons Attribution License, permitting unrestricted use, 
      distribution, and reproduction so long as the original work is properly cited. ( 
      https://creativecommons.org/licenses/by/4.0/ ).
FAU - Qiu, Xin
AU  - Qiu X
AD  - Department of Otolaryngology-Head & Neck Surgery, The Sixth Medical Center of PLA 
      General Hospital, National Clinical Research Center for Otolaryngologic Diseases, 
      State Key Lab of Hearing Science, Ministry of Education, Beijing Key Lab of 
      Hearing Impairment Prevention and Treatment Beijing, Beijing, People's Republic 
      of China.
FAU - Jiang, Qing-Qing
AU  - Jiang QQ
AD  - Department of Otolaryngology-Head & Neck Surgery, The Sixth Medical Center of PLA 
      General Hospital, National Clinical Research Center for Otolaryngologic Diseases, 
      State Key Lab of Hearing Science, Ministry of Education, Beijing Key Lab of 
      Hearing Impairment Prevention and Treatment Beijing, Beijing, People's Republic 
      of China.
FAU - Guo, Wei-Wei
AU  - Guo WW
AD  - Department of Otolaryngology-Head & Neck Surgery, The Sixth Medical Center of PLA 
      General Hospital, National Clinical Research Center for Otolaryngologic Diseases, 
      State Key Lab of Hearing Science, Ministry of Education, Beijing Key Lab of 
      Hearing Impairment Prevention and Treatment Beijing, Beijing, People's Republic 
      of China.
FAU - Yu, Ning
AU  - Yu N
AD  - Department of Otolaryngology-Head & Neck Surgery, The Sixth Medical Center of PLA 
      General Hospital, National Clinical Research Center for Otolaryngologic Diseases, 
      State Key Lab of Hearing Science, Ministry of Education, Beijing Key Lab of 
      Hearing Impairment Prevention and Treatment Beijing, Beijing, People's Republic 
      of China.
FAU - Yang, Shi-Ming
AU  - Yang SM
AD  - Department of Otolaryngology-Head & Neck Surgery, The Sixth Medical Center of PLA 
      General Hospital, National Clinical Research Center for Otolaryngologic Diseases, 
      State Key Lab of Hearing Science, Ministry of Education, Beijing Key Lab of 
      Hearing Impairment Prevention and Treatment Beijing, Beijing, People's Republic 
      of China.
LA  - eng
PT  - Journal Article
DEP - 20231204
PL  - Germany
TA  - Glob Med Genet
JT  - Global medical genetics
JID - 101769583
PMC - PMC10695707
OTO - NOTNLM
OT  - biomarkers
OT  - gentamicin
OT  - hearing loss
OT  - noise
OT  - proteomics
OT  - transcriptomics
COIS- Conflict of Interest The authors declare that the research was conducted without 
      any commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/12/06 06:42
MHDA- 2023/12/06 06:43
PMCR- 2023/12/01
CRDT- 2023/12/06 04:03
PHST- 2023/12/06 06:43 [medline]
PHST- 2023/12/06 06:42 [pubmed]
PHST- 2023/12/06 04:03 [entrez]
PHST- 2023/12/01 00:00 [pmc-release]
AID - GMG-D-23-00070 [pii]
AID - 10.1055/s-0043-1777069 [doi]
PST - epublish
SO  - Glob Med Genet. 2023 Dec 4;10(4):357-369. doi: 10.1055/s-0043-1777069. 
      eCollection 2023 Dec.