PMID- 38056570
OWN - NLM
STAT- MEDLINE
DCOM- 20240422
LR  - 20240422
IS  - 1525-3198 (Electronic)
IS  - 0022-0302 (Linking)
VI  - 107
IP  - 5
DP  - 2024 May
TI  - Single-step genome-wide association analyses for milk urea concentration in 
      Walloon Holstein cows.
PG  - 3020-3031
LID - S0022-0302(23)00825-1 [pii]
LID - 10.3168/jds.2023-23902 [doi]
AB  - The aims of this study were to conduct a single-step genome-wide association to 
      identify genomic regions associated with milk urea (MU) and to estimate genetic 
      correlations between MU and milk yield (MY), milk composition (calcium content 
      [CC], fat percentage [FP], protein percentage [PP], and casein percentage [CNP]), 
      and cheese-making properties (CMP; coagulation time [CT], curd firmness after 30 
      min from rennet addition [a30], and titratable acidity [TA]). The used data have 
      been collected from 2015 to 2020 on 78,073 first-parity (485,218 test-day 
      records) and 48,766 second-parity (284,942 test-day records) Holstein cows 
      distributed in 671 herds in the Walloon Region of Belgium. Data of 565,533 SNP 
      located on 29 BTA of 6,617 animals (1,712 males) were used. Random regression 
      test-day models were used to estimate genetic parameters through the Bayesian 
      Gibbs sampling method. The SNP solutions were estimated using a single-step 
      genomic BLUP approach. The proportion of the total additive genetic variance 
      explained by windows of 50 consecutive SNPs (with an average size of  approximately 216 kb) was 
      calculated, and the top-3 genomic regions explaining the largest rate of the 
      genetic variance were considered promising regions and used to identify potential 
      candidate genes. Mean (SD) MU was 25.38 (8.02) mg/dL and 25.03 (8.06) mg/dL in 
      the first and second lactation, respectively. Mean heritability estimates for 
      daily MU were 0.21 and 0.23 for the first and second lactation, respectively. The 
      genetic correlations estimated between MU and MY, milk composition, and CMP were 
      quite low (ranged from -0.10 [CC] to 0.10 [TA] and -0.05 [CT] to 0.13 [TA] for 
      the first and second lactations, respectively). The top-3 regions associated with 
      MU were located from 80.61 to 80.74 Mb on BTA6, 103.26 to 103.41 Mb on BTA11, and 
      1.59 to 2.15 Mb on BTA14. Genes including KCNT1, MROH1, SHARPIN, TSSK5, CPSF1, 
      HSF1, TONSL, DGAT1, SCX, and MAF1 were identified as positional candidate genes 
      for MU. The findings of this study can be used for a better understanding of the 
      genomic architecture underlying MU in Holstein cattle.
CI  - The Authors. Published by Elsevier Inc. on behalf of the American Dairy Science 
      Association(R). This is an open access article under the CC BY license 
      (http://creativecommons.org/licenses/by/4.0/).
FAU - Atashi, H
AU  - Atashi H
AD  - TERRA Teaching and Research Center, Gembloux Agro-Bio Tech, University of Liege, 
      5030 Gembloux, Belgium; Department of Animal Science, Shiraz University, 
      71441-13131 Shiraz, Iran. Electronic address: atashi@shirazu.ac.ir.
FAU - Chen, Y
AU  - Chen Y
AD  - TERRA Teaching and Research Center, Gembloux Agro-Bio Tech, University of Liege, 
      5030 Gembloux, Belgium.
FAU - Vanderick, S
AU  - Vanderick S
AD  - TERRA Teaching and Research Center, Gembloux Agro-Bio Tech, University of Liege, 
      5030 Gembloux, Belgium.
FAU - Hubin, X
AU  - Hubin X
AD  - Eleveo asbl Awe Group, 5590 Ciney, Belgium.
FAU - Gengler, N
AU  - Gengler N
AD  - TERRA Teaching and Research Center, Gembloux Agro-Bio Tech, University of Liege, 
      5030 Gembloux, Belgium.
LA  - eng
PT  - Journal Article
DEP - 20231204
PL  - United States
TA  - J Dairy Sci
JT  - Journal of dairy science
JID - 2985126R
RN  - 8W8T17847W (Urea)
SB  - IM
MH  - Pregnancy
MH  - Female
MH  - Male
MH  - Cattle/genetics
MH  - Animals
MH  - *Milk/metabolism
MH  - *Genome-Wide Association Study/veterinary
MH  - Urea/metabolism
MH  - Bayes Theorem
MH  - Lactation/genetics
MH  - Phenotype
OTO - NOTNLM
OT  - Holstein
OT  - genomic association
OT  - milk urea
EDAT- 2023/12/07 00:42
MHDA- 2024/04/22 06:42
CRDT- 2023/12/06 19:23
PHST- 2023/06/26 00:00 [received]
PHST- 2023/11/07 00:00 [accepted]
PHST- 2024/04/22 06:42 [medline]
PHST- 2023/12/07 00:42 [pubmed]
PHST- 2023/12/06 19:23 [entrez]
AID - S0022-0302(23)00825-1 [pii]
AID - 10.3168/jds.2023-23902 [doi]
PST - ppublish
SO  - J Dairy Sci. 2024 May;107(5):3020-3031. doi: 10.3168/jds.2023-23902. Epub 2023 
      Dec 4.