PMID- 38057100
OWN - NLM
STAT- MEDLINE
DCOM- 20240130
LR  - 20240206
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 47
IP  - 1
DP  - 2024 Jan 26
TI  - Tanshinone IIA Alleviates Early Brain Injury after Subarachnoid Hemorrhage in 
      Rats by Inhibiting the Activation of NF-kappaB/NLRP3 Inflammasome.
PG  - 279-291
LID - 10.1248/bpb.b23-00519 [doi]
AB  - The abnormal activation of the nuclear factor-kappa B (NF-kappaB)/nod-like receptor 
      family-pyrin domain-containing 3 (NLRP3) signaling pathway is closely related to 
      early brain injury after subarachnoid hemorrhage (SAH). Targeting the 
      NLRP3-inflammasome has been considered an efficient therapy for the local 
      inflammatory response after SAH. Tanshinone IIA (Tan IIA), a major component 
      extracted from Salvia miltiorrhiza, has been reported to have anti-inflammatory 
      effects. The aim of this study was to investigate the effect and mechanism of Tan 
      IIA on early brain injury after SAH. In vivo SAH injury was established by 
      endovascular perforation technique in Sprague-Dawley rats. Limb-placement test 
      and corner turning test were used to measure the behavior. Terminal 
      deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling 
      (TUNEL) staining, hematoxylin-eosin (H&E) staining, and immunofluorescence were 
      used to evaluate the nerve damage. Real-time RT quantitative PCR (RT-qPCR) was 
      used to quantify the levels of inflammatory factors. Western blot was performed 
      for the activation of the NF-kappaB/NLRP3 pathway. An in vitro SAH model was used to 
      validate the conclusion. We found that the neurobehavioral impairment and 
      cerebral edema in SAH model rats given Tan IIA were alleviated. Further study 
      demonstrated that Tan IIA could inhibit SAH-secondary neuronal apoptosis around 
      hematoma and alleviate brain injury. Tan IIA down-regulated the expression of 
      interleukin-6 (IL)-6, monocyte chemoattractant protein-1 (MCP-1), and tumor 
      necrosis factor (TNF)-alpha, and inhibited the activation of NF-kappaB. And the 
      overexpression of pro-inflammatory factors NLRP3, IL-1beta, and IL-18 induced after 
      SAH was also reversed by Tan IIA. In conclusions, Tan IIA could inhibit the 
      NF-kappaB/NLRP3 inflammasome activation to protect and ameliorate SAH-followed early 
      brain injury, and may be a preventive and therapeutic strategy against SAH.
FAU - Yang, Fanhui
AU  - Yang F
AD  - Department of Nuclear Medicine, The Affiliated Hospital of North Sichuan Medical 
      College.
FAU - Ma, Ningshuai
AU  - Ma N
AD  - Department of Ultrasonography, The Affiliated Hospital of North Sichuan Medical 
      College.
FAU - Li, Suping
AU  - Li S
AD  - Department of Nuclear Medicine, The Affiliated Hospital of North Sichuan Medical 
      College.
FAU - Chen, Fei
AU  - Chen F
AD  - Department of Nuclear Medicine, The Affiliated Hospital of North Sichuan Medical 
      College.
FAU - Huang, Xiaohong
AU  - Huang X
AD  - Department of Nuclear Medicine, The Affiliated Hospital of North Sichuan Medical 
      College.
FAU - Zhao, Li
AU  - Zhao L
AD  - Department of Neurology, The Affiliated Hospital of North Sichuan Medical 
      College.
AD  - Institute of Neurological Diseases, North Sichuan Medical College.
FAU - Cao, Lingzhi
AU  - Cao L
AD  - Department of Nuclear Medicine, The Affiliated Hospital of North Sichuan Medical 
      College.
LA  - eng
PT  - Journal Article
DEP - 20231206
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Inflammasomes)
RN  - 0 (NF-kappa B)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 03UUH3J385 (tanshinone)
RN  - 0 (Abietanes)
SB  - IM
MH  - Rats
MH  - Animals
MH  - Inflammasomes/metabolism
MH  - NF-kappa B/metabolism
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
MH  - *Subarachnoid Hemorrhage/complications/drug therapy/pathology
MH  - Rats, Sprague-Dawley
MH  - *Brain Injuries/pathology
MH  - *Abietanes
OTO - NOTNLM
OT  - early brain injury
OT  - neuron
OT  - nod-like receptor family-pyrin domain-containing 3 (NLRP3)
OT  - nuclear factor-kappa B (NF-kappaB)
OT  - subarachnoid hemorrhage
OT  - tanshinone IIA
EDAT- 2023/12/07 00:42
MHDA- 2024/01/30 12:42
CRDT- 2023/12/06 21:03
PHST- 2024/01/30 12:42 [medline]
PHST- 2023/12/07 00:42 [pubmed]
PHST- 2023/12/06 21:03 [entrez]
AID - 10.1248/bpb.b23-00519 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2024 Jan 26;47(1):279-291. doi: 10.1248/bpb.b23-00519. Epub 2023 
      Dec 6.