PMID- 38061253
OWN - NLM
STAT- MEDLINE
DCOM- 20240214
LR  - 20240214
IS  - 1878-0407 (Electronic)
IS  - 1744-117X (Linking)
VI  - 49
DP  - 2024 Mar
TI  - Comparative proteomic profiling represents an inhibition of protein synthesis to 
      regulate osmotic stress in Nile tilapia (Oreochromis niloticus) embryos.
PG  - 101173
LID - S1744-117X(23)00118-1 [pii]
LID - 10.1016/j.cbd.2023.101173 [doi]
AB  - Seawater (SW)-acclimated Nile tilapia, Oreochromis niloticus, can tolerate up to 
      30 g.L(-1) SW but rarely produce offspring. The embryos of SW-acclimated O. 
      niloticus survived equally well from 0- to 10-g.L(-1) environment but not under 
      20-g. L(-1). However, when the embryos were incubated under 10 g.L(-1) during 
      days 0-3, and then the salinity was suddenly shifted to and maintained at 
      20 g.L(-1) during days 4-6, their survival rate was comparable to those incubated 
      under 0 and 10 g.L(-1). To elucidate a molecular adaptation of the embryos that 
      survived different salinity environments, the proteomic profiles of the newly 
      hatched embryos, or early larvae, hatched under 0 g.L(-1), 10 g.L(-1), and those 
      being incubated at 10 g.L(-1) during days 0-3 followed at 20 g.L(-1) during days 
      4-6 were compared. Total proteins extracted from the samples were identified with 
      a gel-free shot-gun proteomics approach using the Nile tilapia protein database. 
      The early larvae from the three groups expressed 2295 proteins, and 279 proteins 
      showed statistically different expressions among groups. Downregulation of the 
      182 proteins in the larvae hatched under 10 and 20 g.L(-1) was found to include 
      22 proteins that are responsible for cellular responses to osmotic stress. This 
      adaptation may be a crucial factor in reducing cellular metabolism and ion 
      transport between the intra- and extra-cellular environment to stabilize cellular 
      osmolality. In addition, some of these proteins suppress cellular damage from 
      oxygen free radicals generated from the osmotic stress. Eighty-seven proteins 
      significantly changed in the larvae hatched under 20 g.L(-1) were clustered. 
      Nineteen of the cellular stress response proteins, which were considered to be 
      mortality induction, were described.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Withyachumnarnkul, Biboon
AU  - Withyachumnarnkul B
AD  - Aquaculture Program, Faculty of Innovative Fisheries Establishment Project, 
      Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, Thailand; 
      AquaAcademy Farm, Tha Chana, Surat Thani 84170, Thailand.
FAU - Pongtippatee, Pattira
AU  - Pongtippatee P
AD  - Aquaculture Program, Faculty of Innovative Fisheries Establishment Project, 
      Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, Thailand.
FAU - Ruangsri, Jareeporn
AU  - Ruangsri J
AD  - Aquaculture Program, Faculty of Innovative Fisheries Establishment Project, 
      Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, Thailand.
FAU - Vanichviriyakit, Rapeepun
AU  - Vanichviriyakit R
AD  - Department of Anatomy, Faculty of Science, Mahidol University, Rama 6 Rd., 
      Bangkok 10400, Thailand; Center of Excellence for Shrimp Molecular Biology and 
      Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Rama 6 
      Rd., Bangkok 10400, Thailand.
FAU - Roytrakul, Sitthiruk
AU  - Roytrakul S
AD  - Functional Proteomics Technology Laboratory, National Center for Genetic 
      Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phaholyothin 
      Rd., Klong Luang, Pathum Thani 12120, Thailand.
FAU - Withyachumnarnkul, Boonsirm
AU  - Withyachumnarnkul B
AD  - Aquaculture Program, Faculty of Innovative Fisheries Establishment Project, 
      Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, Thailand; 
      AquaAcademy Farm, Tha Chana, Surat Thani 84170, Thailand.
FAU - Chotwiwatthanakun, Charoonroj
AU  - Chotwiwatthanakun C
AD  - Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex 
      Shrimp), Faculty of Science, Mahidol University, Rama 6 Rd., Bangkok 10400, 
      Thailand; Mahidol University, Nakhonsawan Campus, Nakhonsawan 60130, Thailand. 
      Electronic address: charoonroj.cho@mahidol.ac.th.
LA  - eng
PT  - Journal Article
DEP - 20231205
PL  - Netherlands
TA  - Comp Biochem Physiol Part D Genomics Proteomics
JT  - Comparative biochemistry and physiology. Part D, Genomics & proteomics
JID - 101270611
SB  - IM
MH  - Animals
MH  - *Cichlids
MH  - Osmotic Pressure
MH  - Proteomics
MH  - Salinity
MH  - Acclimatization
OTO - NOTNLM
OT  - Oreochromis niloticus
OT  - Proteomics
OT  - Salinity-adapted embryos
OT  - Seawater-acclimated Nile tilapia
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/12/08 00:42
MHDA- 2024/02/13 06:45
CRDT- 2023/12/07 18:07
PHST- 2023/08/31 00:00 [received]
PHST- 2023/12/01 00:00 [revised]
PHST- 2023/12/03 00:00 [accepted]
PHST- 2024/02/13 06:45 [medline]
PHST- 2023/12/08 00:42 [pubmed]
PHST- 2023/12/07 18:07 [entrez]
AID - S1744-117X(23)00118-1 [pii]
AID - 10.1016/j.cbd.2023.101173 [doi]
PST - ppublish
SO  - Comp Biochem Physiol Part D Genomics Proteomics. 2024 Mar;49:101173. doi: 
      10.1016/j.cbd.2023.101173. Epub 2023 Dec 5.