PMID- 38061618
OWN - NLM
STAT- MEDLINE
DCOM- 20240103
LR  - 20240106
IS  - 1532-0456 (Print)
IS  - 1532-0456 (Linking)
VI  - 276
DP  - 2024 Feb
TI  - Assessment of metabolic responses following silica nanoparticles in zebrafish 
      models using (1)H NMR analysis.
PG  - 109808
LID - S1532-0456(23)00263-6 [pii]
LID - 10.1016/j.cbpc.2023.109808 [doi]
AB  - Silica nanoparticles (SNPs) are widely explored as drug carriers, gene delivery 
      vehicles, and as nanoparticles intended for bone and tissue engineering. SNPs are 
      highly evident through various clinical trials for a wide range of biomedical 
      applications. SNPs are biocompatible and promising nanoparticles for 
      next-generation therapeutics. However, despite the well-established importance of 
      SNPs, metabolomics methods for the SNPs remain elusive which renders its maximal 
      clinical translation. We applied (1)H nuclear magnetic resonance ((1)H NMR) 
      spectroscopy to investigate the metabolomics profile in Zebrafish (Danio rerio) 
      exposed to SNPs. Zebrafish were exposed to the SNPs (10.0, 25.0, and 50.0 mug/mL) 
      for 72 h and whole-body samples were subjected for targeted profiling. Pattern 
      recognition of (1)H NMR spectral data depicted alterations in the metabolomic 
      profiles between control and SNPs exposed zebrafish. We found that tryptophane, 
      lysine, methionine, phenylalanine, tyrosine, sn-glycero-3-phosphocholine (G3PC), 
      and o-phosphocholine were decreased. The metabolic expression of niacinamide, 
      nicotinamide adenine dinucleotide (NAD+), citrate, adenosine triphosphate (ATP), 
      and xanthine were increased in zebrafish with SNPs treatment. We are report for 
      the first time on metabolite alterations and phenotypic expression in zebrafish 
      via (1)H NMR. These results demonstrate that SNPs can adversely affect the 
      significant metabolic pathways involved in energy, amino acids, cellular 
      membrane, lipids, and fatty acid metabolisms. Metabolomics profiling may be able 
      to detect metabolic dysregulation in SNPs-treated zebrafish and establish a 
      foundation for further toxicological studies.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Govindasamy, Chandramohan
AU  - Govindasamy C
AD  - Department of Community Health Sciences, College of Applied Medical Sciences, 
      King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia.
FAU - Al-Numair, Khalid S
AU  - Al-Numair KS
AD  - Department of Community Health Sciences, College of Applied Medical Sciences, 
      King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia.
FAU - Alsaif, Mohammed A
AU  - Alsaif MA
AD  - Department of Community Health Sciences, College of Applied Medical Sciences, 
      King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia.
FAU - Gopalakrishnan, Abilash Valsala
AU  - Gopalakrishnan AV
AD  - Department of Biomedical Sciences, School of Bio Sciences and Technology, Vellore 
      Institute of Technology (VIT), Vellore, Tamil Nadu 632 014, India.
FAU - Ganesan, Raja
AU  - Ganesan R
AD  - Department of Biotechnology, Faculty of Science and Humanities, SRM Institute of 
      Science and Technology, Kattankulathur, 603 203 Chengalpattu District, Tamil 
      Nadu, India. Electronic address: ganesanr2@srmist.edu.in.
LA  - eng
PT  - Journal Article
DEP - 20231206
PL  - United States
TA  - Comp Biochem Physiol C Toxicol Pharmacol
JT  - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
JID - 100959500
RN  - 107-73-3 (Phosphorylcholine)
SB  - IM
MH  - Animals
MH  - *Zebrafish/metabolism
MH  - *Phosphorylcholine/metabolism
MH  - Proton Magnetic Resonance Spectroscopy
MH  - Magnetic Resonance Spectroscopy
MH  - Metabolomics/methods
OTO - NOTNLM
OT  - (1)H NMR
OT  - Chemometrics
OT  - Metabolic syndrome
OT  - Metabolomics
OT  - Silica nanoparticles
OT  - Zebrafish
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/12/08 00:42
MHDA- 2024/01/03 09:42
CRDT- 2023/12/07 19:28
PHST- 2023/09/26 00:00 [received]
PHST- 2023/11/14 00:00 [revised]
PHST- 2023/11/30 00:00 [accepted]
PHST- 2024/01/03 09:42 [medline]
PHST- 2023/12/08 00:42 [pubmed]
PHST- 2023/12/07 19:28 [entrez]
AID - S1532-0456(23)00263-6 [pii]
AID - 10.1016/j.cbpc.2023.109808 [doi]
PST - ppublish
SO  - Comp Biochem Physiol C Toxicol Pharmacol. 2024 Feb;276:109808. doi: 
      10.1016/j.cbpc.2023.109808. Epub 2023 Dec 6.