PMID- 38062819
OWN - NLM
STAT- MEDLINE
DCOM- 20231216
LR  - 20240220
IS  - 2768-6698 (Electronic)
IS  - 2768-6698 (Linking)
VI  - 28
IP  - 11
DP  - 2023 Nov 28
TI  - Unveiling Circular RNA-Mediated Regulatory Mechanisms in Necroptosis in Premature 
      Ovarian Failure.
PG  - 314
LID - 10.31083/j.fbl2811314 [doi]
AB  - BACKGROUND: Necroptosis is a programmed necrotic cell death, in which dying cells 
      rupture and release intracellular components that trigger a proinflammatory 
      response. The current study aimed at probing the circular RNA (circRNA)-mediated 
      regulatory mechanisms in necroptosis in premature ovarian failure (POF). METHODS: 
      CircRNA sequencing analysis was conducted in ovarian tissues of control and POF 
      rats and transcriptome microarrays were acquired from the GSE33423 dataset. 
      Differential expression analysis of circRNAs and mRNAs was executed between the 
      POF and control data. Both a necroptosis-based circRNA-microRNA (miRNA)-mRNA 
      network and a protein-protein interaction (PPI) network were established. Then, 
      the functional annotation and immunological traits were analyzed. RESULTS: 
      Totally, 1266 upregulated and 1283 downregulated circRNAs as well as 1101 
      upregulated and 1168 downregulated mRNAs were determined in the POF rats versus 
      the controls. The differentially expressed mRNAs predominantly correlated with 
      necroptosis. The circRNA-miRNA-mRNA networks of downregulated necroptosis genes 
      (comprising rno_circRNA_004995-rno-miR-148b-5p-H2afy2, 
      rno_circRNA_016998-rno-miR-29a-5p-Hmgb1, and 
      rno_circRNA_017593-rno-miR-29a-5p-Hmgb1) and upregulated necroptosis genes 
      (comprising rno_circRNA_015900-rno-miR-935-Stat1, 
      rno_circRNA_007946-rno-miR-328a-3p-Stat5a, 
      rno_circRNA_007947-rno-miR-328a-3p-Stat5a, 
      rno_circRNA_005064-rno-miR-18a-5p-Stat1, 
      rno_circRNA_005064-rno-miR-18a-5p-Stat5a, rno_circRNA_005115-rno-miR-22-3p-Stat1, 
      rno_circRNA_009028-rno-miR-342-5p-Stat1, rno_circRNA_011240-rno-miR-1224-Stat5a, 
      rno_circRNA_016078-rno-miR-711-Stat5a) were built. POF-specific necroptosis genes 
      (STAT1, STAT5A, PLA2G4A, HMG1L1, HMGB1, AGER, EGFR, HDAC7, IFNA1, IL10RB, IL27RA, 
      PYGL, SOCS1, TRADD, CXCL10, DDX5, EZH2, FADS2, FER, H2AFY2, HIST1H2AF, IFI44L, 
      IL27, IRGM, MX1, NFKB2, PAFAH2, PEMT, PGM2L1, PGR, PHKA2, and PLB1) were selected 
      since they displayed notable associations with most immune cells, immune 
      checkpoints, chemokines, human leukocyte antigen (HLA) molecules, and immune 
      receptors. CONCLUSIONS: Altogether, we proposed the presence of widespread 
      regulatory mechanisms of circRNAs in necroptosis and demonstrated that altered 
      circRNA biogenesis might contribute to POF by affecting necroptosis.
CI  - (c) 2023 The Author(s). Published by IMR Press.
FAU - Jin, Xin
AU  - Jin X
AD  - Department of Tuina, The First Affiliated Hospital of Zhejiang Chinese Medical 
      University, 310006 Hangzhou, Zhejiang, China.
FAU - Chen, Wenjun
AU  - Chen W
AD  - School of Nursing, Hangzhou Medical College, 311399 Hangzhou, Zhejiang, China.
AD  - Department of Gynecology and Obstetrics, The First Affiliated Hospital of 
      Zhejiang Chinese Medical University, 310006 Hangzhou, Zhejiang, China.
FAU - Wang, Jiaxi
AU  - Wang J
AD  - Department of Gynecology and Obstetrics, The First Affiliated Hospital of 
      Zhejiang Chinese Medical University, 310006 Hangzhou, Zhejiang, China.
FAU - Xu, Xianli
AU  - Xu X
AD  - Department of Gynecology and Obstetrics, The First Affiliated Hospital of 
      Zhejiang Chinese Medical University, 310006 Hangzhou, Zhejiang, China.
FAU - Zhang, Ting
AU  - Zhang T
AD  - Department of Gynecology and Obstetrics, The First Affiliated Hospital of 
      Zhejiang Chinese Medical University, 310006 Hangzhou, Zhejiang, China.
FAU - Wang, Lu
AU  - Wang L
AD  - Department of Lung Disease, Jining Hospital of Traditional Chinese Medicine, 
      272037 Jining, Shandong, China.
FAU - Feng, Xuehua
AU  - Feng X
AD  - Center of Reproductive Medicine, The Second Hospital Affiliated to Shandong 
      University of Traditional Chinese Medicine, 250001 Jinan, Shandong, China.
LA  - eng
GR  - 82004405/National Natural Science Foundation of China/
GR  - LQ20H270005/Exploration Project of Natural Science Foundation of Zhejiang 
      Province/
GR  - 2023ZL366/Zhejiang Province Traditional Chinese Medicine Science and technology 
      Project/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Singapore
TA  - Front Biosci (Landmark Ed)
JT  - Frontiers in bioscience (Landmark edition)
JID - 101612996
RN  - 0 (RNA, Circular)
RN  - 0 (HMGB1 Protein)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.1.19 (PHKA2 protein, human)
RN  - EC 2.7.1.19 (Phosphorylase Kinase)
RN  - 0 (MIRN1224 microRNA, human)
RN  - 0 (MIRN711 microRNA, rat)
SB  - IM
MH  - Female
MH  - Humans
MH  - Rats
MH  - Animals
MH  - RNA, Circular/genetics
MH  - *HMGB1 Protein
MH  - *Primary Ovarian Insufficiency/genetics
MH  - Necroptosis/genetics
MH  - *MicroRNAs/genetics/metabolism
MH  - RNA, Messenger/genetics
MH  - Phosphorylase Kinase
OTO - NOTNLM
OT  - circRNA
OT  - circRNA-miRNA-mRNA network
OT  - immunity
OT  - necroptosis
OT  - premature ovarian failure
OT  - protein-protein interaction
COIS- The authors declare no conflict of interest.
EDAT- 2023/12/08 06:42
MHDA- 2023/12/17 13:19
CRDT- 2023/12/08 03:42
PHST- 2023/02/24 00:00 [received]
PHST- 2023/06/15 00:00 [revised]
PHST- 2023/07/07 00:00 [accepted]
PHST- 2023/12/17 13:19 [medline]
PHST- 2023/12/08 06:42 [pubmed]
PHST- 2023/12/08 03:42 [entrez]
AID - S2768-6701(23)00956-5 [pii]
AID - 10.31083/j.fbl2811314 [doi]
PST - ppublish
SO  - Front Biosci (Landmark Ed). 2023 Nov 28;28(11):314. doi: 10.31083/j.fbl2811314.