PMID- 38062832
OWN - NLM
STAT- MEDLINE
DCOM- 20231216
LR  - 20240220
IS  - 2768-6698 (Electronic)
IS  - 2768-6698 (Linking)
VI  - 28
IP  - 11
DP  - 2023 Nov 29
TI  - Aspirin Inhibits Brain Metastasis of Lung Cancer via Upregulation of Tight 
      Junction Protein Expression in Microvascular Endothelial Cells.
PG  - 320
LID - 10.31083/j.fbl2811320 [doi]
AB  - BACKGROUND: The brain is one of the most vulnerable metastasis sites in lung 
      cancer; approximately 40-50% of lung cancer patients develop brain metastasis 
      during the disease course, contributing to the poor prognosis and high mortality 
      of lung cancer patients. Therefore, it is important to clarify the molecular 
      mechanism underlying brain metastasis of lung cancer for improving the overall 
      survival of lung cancer patients. The present study aimed to investigate the 
      potential role of blood-brain barrier (BBB) permeability in the development of 
      brain metastasis of lung cancer and explore the effect of aspirin in an in-vitro 
      BBB model. METHODS: An in-vitro BBB model was established. The expression of heat 
      shock protein 70 (HSP 70), zonula occludens-1 (ZO-1), and occludin in rat brain 
      microvascular endothelial cells was detected using Western blot at different time 
      points following the administration of aspirin. RESULTS: HSP70, ZO-1, and 
      occludin expressions did not show significant changes before aspirin 
      administration, but increased noticeably after aspirin administration. Tumor 
      necrosis factor-alpha (TNF-alpha) could significantly attenuate the increased expression 
      of these proteins induced by aspirin. Additionally, TNF-alpha also significantly 
      reversed the aspirin-induced decrease of BBB permeability. CONCLUSIONS: Aspirin 
      may inhibit brain metastasis of lung cancer in a time-dependent manner via 
      upregulating tight junction proteins to reduce BBB permeability, and this effect 
      can be reversed by TNF-alpha.
CI  - (c) 2023 The Author(s). Published by IMR Press.
FAU - Wei, Dianfang
AU  - Wei D
AD  - Department of Basic Medicine, Qilu Medical University, 255300 Zibo, Shandong, 
      China.
FAU - Tang, Ming
AU  - Tang M
AD  - Department of Basic Medicine, Qilu Medical University, 255300 Zibo, Shandong, 
      China.
FAU - Gong, Weibo
AU  - Gong W
AD  - Department of Basic Medicine, Qilu Medical University, 255300 Zibo, Shandong, 
      China.
FAU - Liu, Jingshuo
AU  - Liu J
AD  - Department of Basic Medicine, Qilu Medical University, 255300 Zibo, Shandong, 
      China.
FAU - Qin, Lijuan
AU  - Qin L
AD  - School of Basic Medical Sciences, North China University of Science and 
      Technology, 063210 Tangshan, Hebei, China.
LA  - eng
GR  - 81101912/Natural Science Foundation of China/
GR  - ZD20160082/Science and Technology Project for Colleges and Universities in Hebei 
      Province/
GR  - 20170901/Scientific Research Foundation of Hebei Health Department/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Singapore
TA  - Front Biosci (Landmark Ed)
JT  - Frontiers in bioscience (Landmark edition)
JID - 101612996
RN  - 0 (Tight Junction Proteins)
RN  - 0 (Occludin)
RN  - R16CO5Y76E (Aspirin)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Rats
MH  - Animals
MH  - Humans
MH  - Tight Junction Proteins/metabolism
MH  - Occludin/genetics/metabolism/pharmacology
MH  - Endothelial Cells/metabolism
MH  - Up-Regulation
MH  - *Lung Neoplasms/drug therapy/metabolism
MH  - Aspirin/pharmacology
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Blood-Brain Barrier
MH  - *Brain Neoplasms/drug therapy/metabolism
MH  - Tight Junctions/metabolism
OTO - NOTNLM
OT  - aspirin
OT  - blood-brain barrier
OT  - brain metastasis
OT  - lung cancer
OT  - tight junction protein
COIS- The authors declare no conflict of interest.
EDAT- 2023/12/08 06:42
MHDA- 2023/12/17 13:19
CRDT- 2023/12/08 03:43
PHST- 2022/09/06 00:00 [received]
PHST- 2023/06/16 00:00 [revised]
PHST- 2023/08/08 00:00 [accepted]
PHST- 2023/12/17 13:19 [medline]
PHST- 2023/12/08 06:42 [pubmed]
PHST- 2023/12/08 03:43 [entrez]
AID - S2768-6701(23)00997-8 [pii]
AID - 10.31083/j.fbl2811320 [doi]
PST - ppublish
SO  - Front Biosci (Landmark Ed). 2023 Nov 29;28(11):320. doi: 10.31083/j.fbl2811320.