PMID- 38063100
OWN - NLM
STAT- MEDLINE
DCOM- 20240105
LR  - 20240105
IS  - 1165-158X (Electronic)
IS  - 0145-5680 (Linking)
VI  - 69
IP  - 12
DP  - 2023 Nov 30
TI  - Low expression of LncRNA AFAP1-AS1 inhibits proliferation and promotes apoptosis 
      of non-small cell lung cancer cells through inhibiting wnt signaling pathway.
PG  - 176-180
LID - 10.14715/cmb/2023.69.12.28 [doi]
AB  - To detect the effects of long non-coding ribonucleic acid (lncRNA) actin 
      filament-associated protein 1-antisense RNA1 (AFAP1-AS1) on the proliferation and 
      apoptosis of non-small cell lung cancer (NSCLC) A549 cells and its mechanism. 1) 
      The expression of lncRNA AFAP1-AS1 in NSCLC A549 cells was detected via 
      quantitative reverse transcription-polymerase chain reaction (qRT-PCR). 2) The 
      changes in proliferation and apoptosis of A549 cells after low expression of 
      lncRNA AFAP1-AS1 were detected using cell counting kit-8 (CCK-8) and terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assays. 3) 
      The changes in Wnt signaling pathway proteins in A549 cells after low expression 
      of lncRNA AFAP1-AS1 were detected using Western blotting. 1) The expression of 
      lncRNA AFAP1-AS1 rose in A549 cells (P<0.01). 2) After low expression of lncRNA 
      AFAP1-AS1, the growth of A549 cells was inhibited, and apoptosis was promoted. 3) 
      After low expression of lncRNA AFAP1-AS1, the mRNA and protein expressions of 
      glycogen synthase kinase (GSK) and beta-catenin declined (P<0.05). Lowly-expressed 
      AFAP1-AS1 inhibits the proliferation and promotes the apoptosis of NSCLC A549 
      cells via inhibiting the Wnt signaling pathway.
FAU - Sun, Ying
AU  - Sun Y
AD  - Department of Clinical Laboratory, Tianjin Medical University General Hospital, 
      Tianjin 300052, China. stella20100616@163.com.
FAU - Fan, Liming
AU  - Fan L
AD  - Department of Clinical Laboratory, Tianjin Medical University General Hospital, 
      Tianjin 300052, China. flmtjmugh@126.com.
FAU - Li, Yixian
AU  - Li Y
AD  - Department of Pathology, Tianjin Medical University General Hospital, Tianjin 
      300052, China. liyixian89@163.com.
LA  - eng
PT  - Journal Article
DEP - 20231130
PL  - France
TA  - Cell Mol Biol (Noisy-le-grand)
JT  - Cellular and molecular biology (Noisy-le-Grand, France)
JID - 9216789
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (AFAP1-AS1 long noncoding RNA, human)
SB  - IM
MH  - Humans
MH  - Apoptosis/genetics
MH  - *Carcinoma, Non-Small-Cell Lung/genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - *Lung Neoplasms/genetics
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - *Wnt Signaling Pathway/genetics
EDAT- 2023/12/08 06:41
MHDA- 2023/12/17 13:19
CRDT- 2023/12/08 05:36
PHST- 2023/08/09 00:00 [received]
PHST- 2023/12/17 13:19 [medline]
PHST- 2023/12/08 06:41 [pubmed]
PHST- 2023/12/08 05:36 [entrez]
AID - 10.14715/cmb/2023.69.12.28 [doi]
PST - epublish
SO  - Cell Mol Biol (Noisy-le-grand). 2023 Nov 30;69(12):176-180. doi: 
      10.14715/cmb/2023.69.12.28.