PMID- 38065230
OWN - NLM
STAT- MEDLINE
DCOM- 20240322
LR  - 20240322
IS  - 1090-2392 (Electronic)
IS  - 0011-2240 (Linking)
VI  - 114
DP  - 2024 Mar
TI  - Effects of intracellular Ca(2+) on developmental potential and ultrastructure of 
      cryopreserved-warmed oocyte in mouse.
PG  - 104834
LID - S0011-2240(23)00333-4 [pii]
LID - 10.1016/j.cryobiol.2023.104834 [doi]
AB  - Maintaining appropriate intracellular calcium of oocytes is necessary to prevent 
      ultrastructure and organelle damage caused by freezing and cryoprotectants. The 
      present study aimed to investigate whether cryoprotectant-induced changes in the 
      calcium concentrations of oocytes can be regulated to reduce damage to 
      developmental potential and ultrastructure. A total of 33 mice and 1381 oocytes 
      were used to explore the effects of intracellular calcium on the development and 
      ultrastructures of oocytes subjected to 2-aminoethoxydiphenyl borate (2-APB) 
      inhibition or thapsigargin (TG) stimulation. Results suggested that high levels 
      intracellular calcium interfered with TG compromised oocyte survival (84.4 % vs. 
      93.4 %, p < 0.01) and blastocyst formation in fresh and cryopreservation oocytes 
      (78.1 % vs. 86.4 %, and 60.5 % vs. 72.5 %, p < 0.05) compared with that of 2-APB 
      pretreated oocytes in which Ca(2+) was stabilized even though no differences in 
      fertilization and cleavage was detected (p > 0.05). Examination by transmission 
      electron microscopy indicated that the microvilli decreased and shortened, 
      cortical granules considerably decreased in the cortex area, mitochondrial 
      vesicles and vacuoles increased, and the proportion of vacuole mitochondria 
      increased after oocytes were exposed to cryoprotectants. The 
      cryopreservation-warming process deteriorated the negative effects on organelles 
      of survival oocytes. By contrast, a low level of intracellular calcium mediated 
      with 2-APB was supposed to contribute to the protection of organelles. These 
      findings suggested oocyte injuries induced by cryoprotectants and low 
      temperatures can be alleviated. More studies are necessary to confirm the 
      relationship among Ca(2+) concentration of the cytoplasm, ultrastructural 
      injuries, and disrupted developmental potential in oocytes subjected to 
      cryopreservation and warming.
CI  - Copyright (c) 2023. Published by Elsevier Inc.
FAU - Cheng, Junping
AU  - Cheng J
AD  - Reproductive Medical and Genetic Center, Academy of Medical Sciences of Guangxi 
      Autonomous Region, People's Hospital of Guangxi Autonomous Region, Nanning, 
      530021, China; College of Animal Science and Technology of Guangxi University, 
      Nanning, 530005, China. Electronic address: chengjunpingyy@163.com.
FAU - Wang, Xiaoli
AU  - Wang X
AD  - College of Animal Science and Technology of Guangxi University, Nanning, 530005, 
      China.
FAU - Luo, Chan
AU  - Luo C
AD  - College of Animal Science and Technology of Guangxi University, Nanning, 530005, 
      China.
FAU - Mao, Xianbao
AU  - Mao X
AD  - Reproductive Medical and Genetic Center, Academy of Medical Sciences of Guangxi 
      Autonomous Region, People's Hospital of Guangxi Autonomous Region, Nanning, 
      530021, China.
FAU - Qin, Jie
AU  - Qin J
AD  - Reproductive Medical and Genetic Center, Academy of Medical Sciences of Guangxi 
      Autonomous Region, People's Hospital of Guangxi Autonomous Region, Nanning, 
      530021, China.
FAU - Chi, Yan
AU  - Chi Y
AD  - Reproductive Medical and Genetic Center, Academy of Medical Sciences of Guangxi 
      Autonomous Region, People's Hospital of Guangxi Autonomous Region, Nanning, 
      530021, China.
FAU - He, Bing
AU  - He B
AD  - Reproductive Medical and Genetic Center, Academy of Medical Sciences of Guangxi 
      Autonomous Region, People's Hospital of Guangxi Autonomous Region, Nanning, 
      530021, China.
FAU - Hao, Yanrong
AU  - Hao Y
AD  - Reproductive Medical and Genetic Center, Academy of Medical Sciences of Guangxi 
      Autonomous Region, People's Hospital of Guangxi Autonomous Region, Nanning, 
      530021, China.
FAU - Niu, Xiangli
AU  - Niu X
AD  - Reproductive Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, 
      530021, China.
FAU - Huang, Ben
AU  - Huang B
AD  - Reproductive Medical and Genetic Center, Academy of Medical Sciences of Guangxi 
      Autonomous Region, People's Hospital of Guangxi Autonomous Region, Nanning, 
      530021, China; College of Animal Science and Technology of Guangxi University, 
      Nanning, 530005, China.
FAU - Liu, Liling
AU  - Liu L
AD  - Reproductive Medical and Genetic Center, Academy of Medical Sciences of Guangxi 
      Autonomous Region, People's Hospital of Guangxi Autonomous Region, Nanning, 
      530021, China. Electronic address: 245130379@qq.com.
LA  - eng
PT  - Journal Article
DEP - 20231207
PL  - Netherlands
TA  - Cryobiology
JT  - Cryobiology
JID - 0006252
RN  - SY7Q814VUP (Calcium)
RN  - 0 (Cryoprotective Agents)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Cryopreservation/methods
MH  - *Calcium/pharmacology
MH  - Oocytes
MH  - Freezing
MH  - Cryoprotective Agents/pharmacology
OTO - NOTNLM
OT  - 2-Aminoethoxydiphenyl borate
OT  - Calcium concentration
OT  - Cryopreservation
OT  - Oocyte
OT  - Ultrastructure
EDAT- 2023/12/09 05:43
MHDA- 2024/03/22 06:45
CRDT- 2023/12/08 19:24
PHST- 2023/07/12 00:00 [received]
PHST- 2023/10/15 00:00 [revised]
PHST- 2023/12/01 00:00 [accepted]
PHST- 2024/03/22 06:45 [medline]
PHST- 2023/12/09 05:43 [pubmed]
PHST- 2023/12/08 19:24 [entrez]
AID - S0011-2240(23)00333-4 [pii]
AID - 10.1016/j.cryobiol.2023.104834 [doi]
PST - ppublish
SO  - Cryobiology. 2024 Mar;114:104834. doi: 10.1016/j.cryobiol.2023.104834. Epub 2023 
      Dec 7.