PMID- 38067012
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231209
IS  - 2076-2615 (Print)
IS  - 2076-2615 (Electronic)
IS  - 2076-2615 (Linking)
VI  - 13
IP  - 23
DP  - 2023 Nov 27
TI  - Enterocytozoon hepatopenaei (EHP) Infection Alters the Metabolic Processes and 
      Induces Oxidative Stress in Penaeus vannamei.
LID - 10.3390/ani13233661 [doi]
LID - 3661
AB  - Enterocytozoon hepatopenaei (EHP) is highly contagious and can cause 
      hepatopancreatic microsporidiosis (HPM), which is typically characterized by the 
      slow growth of shrimp. In this study, the differences in histology, metabolism, 
      oxidative stress and growth between healthy and EHP-infected Penaeus vannamei 
      were analyzed using an EHP challenge experiment. Histology showed that EHP caused 
      lesions in the hepatic tubules of P. vannamei, such as hepatic tubular atrophy 
      and epithelial cell shedding, with mature spores. Meanwhile, white feces may 
      appear when the infection is severe. Furthermore, the content of total protein, 
      glycogen, ATP and glucose in the EHP challenge group was significantly reduced. 
      The qPCR results showed that EHP infection changed the expression of key genes in 
      glucose metabolism, among which hexokinase (HK), phosphofructokinase (PFK), 
      pyruvatekinase (PK), citrate synthase (CS) and isocitric dehydrogenase (IDH) were 
      significantly down-regulated, while phosphoenolpyruvate carboxykinase (PEPCK), 
      fructose bisphosphatase (FBP) and glucose-6-phosphatase (G6P) were significantly 
      up-regulated. Obviously, the expression of growth-related genes was disordered. 
      Simultaneously, the antioxidant genes manganese superoxide dismutase (MnSOD), 
      catalase (CAT), glutathione peroxidase (GPX), glutathione-S-transferases (GST) 
      and nuclear factor E2-related factor2 (Nrf2) were up-regulated to varying degrees 
      in the EHP challenge group, and EHP infection induced significant increases in 
      the oxidative damage products lipid peroxide (LPO) and malondialdehyde (MDA). 
      Ultimately, the shrimp weight of the challenge group was 6.85 +/- 0.86 g, which was 
      significantly lower than that of the control group (8.95 +/- 0.75 g). Taken 
      together, we speculate that EHP changes the substance metabolism and growth 
      process by causing oxidative damage to the hepatopancreas, which may lead to the 
      growth retardation of P. vannamei.
FAU - Cao, Zheng
AU  - Cao Z
AD  - Laboratory of Disease Research of Aquatic Animal, School of Agriculture, Ludong 
      University, Yantai 264025, China.
FAU - Chen, Caiyi
AU  - Chen C
AD  - Laboratory of Disease Research of Aquatic Animal, School of Agriculture, Ludong 
      University, Yantai 264025, China.
FAU - Wang, Cuixia
AU  - Wang C
AD  - Laboratory of Disease Research of Aquatic Animal, School of Agriculture, Ludong 
      University, Yantai 264025, China.
FAU - Li, Ting
AU  - Li T
AUID- ORCID: 0000-0002-0854-9124
AD  - Laboratory of Disease Research of Aquatic Animal, School of Agriculture, Ludong 
      University, Yantai 264025, China.
FAU - Chang, Linrui
AU  - Chang L
AD  - Laboratory of Disease Research of Aquatic Animal, School of Agriculture, Ludong 
      University, Yantai 264025, China.
FAU - Si, Lingjun
AU  - Si L
AUID- ORCID: 0009-0007-0756-8482
AD  - Laboratory of Disease Research of Aquatic Animal, School of Agriculture, Ludong 
      University, Yantai 264025, China.
FAU - Yan, Dongchun
AU  - Yan D
AD  - Laboratory of Disease Research of Aquatic Animal, School of Agriculture, Ludong 
      University, Yantai 264025, China.
LA  - eng
GR  - ZR2023QC050/Natural Science Foundation of Shandong Province/
GR  - 20220022/Start-up funding for scientific research of Ludong University/
PT  - Journal Article
DEP - 20231127
PL  - Switzerland
TA  - Animals (Basel)
JT  - Animals : an open access journal from MDPI
JID - 101635614
PMC - PMC10705197
OTO - NOTNLM
OT  - Penaeus vannamei
OT  - antioxidant defense
OT  - glucose metabolism
OT  - growth performance
OT  - microsporidia
COIS- The authors declare no conflict of interest.
EDAT- 2023/12/09 10:42
MHDA- 2023/12/09 10:43
PMCR- 2023/11/27
CRDT- 2023/12/09 01:02
PHST- 2023/11/07 00:00 [received]
PHST- 2023/11/20 00:00 [revised]
PHST- 2023/11/24 00:00 [accepted]
PHST- 2023/12/09 10:43 [medline]
PHST- 2023/12/09 10:42 [pubmed]
PHST- 2023/12/09 01:02 [entrez]
PHST- 2023/11/27 00:00 [pmc-release]
AID - ani13233661 [pii]
AID - animals-13-03661 [pii]
AID - 10.3390/ani13233661 [doi]
PST - epublish
SO  - Animals (Basel). 2023 Nov 27;13(23):3661. doi: 10.3390/ani13233661.