PMID- 38068956
OWN - NLM
STAT- MEDLINE
DCOM- 20231216
LR  - 20231216
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 23
DP  - 2023 Nov 23
TI  - Immunolocalization of Sphingolipid Catabolism Enzymes along the Nephron: Novel 
      Early Urinary Biomarkers of Renal Damage.
LID - 10.3390/ijms242316633 [doi]
LID - 16633
AB  - The objective of this study was to investigate whether the activity of enzymes 
      involved in sphingolipid catabolism could be biomarkers to predict early renal 
      damage in streptozotocin (STZ)-induced diabetic rats and Angiotensin II (Ang 
      II)-induced hypertension rats. Diabetic and hypertensive rats had no changes in 
      plasma creatinine concentration. However, transmission electron microscopy (TEM) 
      analysis showed slight ultrastructural changes in the glomeruli and tubular 
      epithelial cells from diabetic and hypertensive rats. Our results show that the 
      acid sphingomyelinase (aSMase) and neutral sphingomyelinase (nSMase) activity 
      increased in the urine of diabetic rats and decreased in hypertensive rats. Only 
      neutral ceramidase (nCDase) activity increased in the urine of diabetic rats. 
      Furthermore, the immunofluorescence demonstrated positive staining for the 
      nSMase, nCDase, and sphingosine kinase (SphK1) in glomerular mesangial cells, 
      proximal tubule, ascending thin limb of the loop of Henle, thick ascending limb 
      of Henle's loop, and principal cells of the collecting duct in the kidney. In 
      conclusion, our results suggest that aSMase and nCDase activity in urine could be 
      a novel predictor of early slight ultrastructural changes in the nephron, aSMase 
      and nCDase as glomerular injury biomarkers, and nSMase as a tubular injury 
      biomarker in diabetic and hypertensive rats.
FAU - Franco, Martha
AU  - Franco M
AUID- ORCID: 0000-0003-4073-524X
AD  - Department of Cardio-Renal Pathophysiology, Instituto Nacional de Cardiologia 
      "Ignacio Chavez", Mexico City 14080, Mexico.
FAU - Cano-Martinez, Agustina
AU  - Cano-Martinez A
AUID- ORCID: 0000-0002-6703-2176
AD  - Department of Physiology, Instituto Nacional de Cardiologia "Ignacio Chavez", 
      Mexico City 14080, Mexico.
FAU - Ramos-Godinez, Maria Del Pilar
AU  - Ramos-Godinez MDP
AD  - Department of Electron Microscopy, Instituto Nacional de Cancerologia, Mexico 
      City 14080, Mexico.
FAU - Lopez-Marure, Rebeca
AU  - Lopez-Marure R
AD  - Department of Physiology, Instituto Nacional de Cardiologia "Ignacio Chavez", 
      Mexico City 14080, Mexico.
FAU - Donis-Maturano, Luis
AU  - Donis-Maturano L
AD  - Faculty of Higher Studies Iztacala, Universidad Nacional Autonoma de Mexico, 
      Mexico City 54090, Mexico.
FAU - Sosa, Jose Santamaria
AU  - Sosa JS
AD  - Department of Cardio-Renal Pathophysiology, Instituto Nacional de Cardiologia 
      "Ignacio Chavez", Mexico City 14080, Mexico.
FAU - Bautista-Perez, Rocio
AU  - Bautista-Perez R
AUID- ORCID: 0000-0003-0265-668X
AD  - Department of Molecular Biology, Instituto Nacional de Cardiologia "Ignacio 
      Chavez", Mexico City 14080, Mexico.
LA  - eng
GR  - Research Grant A1-S-9870/This project was supported by National Council of 
      Humanities, Sciences and Technologies (CO-NAHCyT) "Project supported by the 
      sectoral research fund for education"/
PT  - Journal Article
DEP - 20231123
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - EC 3.1.4.12 (Sphingomyelin Phosphodiesterase)
RN  - 0 (Sphingolipids)
SB  - IM
MH  - Rats
MH  - Animals
MH  - Sphingomyelin Phosphodiesterase/metabolism
MH  - *Diabetes Mellitus, Experimental/metabolism
MH  - Kidney/metabolism
MH  - Nephrons/metabolism
MH  - *Hypertension
MH  - Sphingolipids
PMC - PMC10706607
OTO - NOTNLM
OT  - angiotensin II-induced hypertension
OT  - ceramidase
OT  - diabetes
OT  - sphingomyelinase
OT  - sphingosine kinase
OT  - urine biomarker
COIS- The authors declare no conflict of interest.
EDAT- 2023/12/09 10:46
MHDA- 2023/12/17 09:44
PMCR- 2023/11/23
CRDT- 2023/12/09 01:13
PHST- 2023/10/13 00:00 [received]
PHST- 2023/11/14 00:00 [revised]
PHST- 2023/11/20 00:00 [accepted]
PHST- 2023/12/17 09:44 [medline]
PHST- 2023/12/09 10:46 [pubmed]
PHST- 2023/12/09 01:13 [entrez]
PHST- 2023/11/23 00:00 [pmc-release]
AID - ijms242316633 [pii]
AID - ijms-24-16633 [pii]
AID - 10.3390/ijms242316633 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Nov 23;24(23):16633. doi: 10.3390/ijms242316633.