PMID- 38069052
OWN - NLM
STAT- MEDLINE
DCOM- 20231216
LR  - 20231216
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 23
DP  - 2023 Nov 24
TI  - Allergenic Activity of Individual Cat Allergen Molecules.
LID - 10.3390/ijms242316729 [doi]
LID - 16729
AB  - More than 10% of the world's population suffers from an immunoglobulin E 
      (IgE)-mediated allergy to cats which is accompanied mainly by respiratory 
      symptoms such as rhinitis and asthma. Several cat allergen molecules have been 
      identified, but their allergenic activity has not been investigated in depth. 
      Purified cat allergen molecules (Fel d 1, Fel d 2, Fel d 3, Fel d 4, Fel d 6, Fel 
      d 7 and Fel d 8) were characterized via mass spectrometry and circular dichroism 
      spectroscopy regarding their molecular mass and fold, respectively. 
      Cat-allergen-specific IgE levels were quantified via ImmunoCAP measurements in 
      IgE-sensitized subjects with (n = 37) and without (n = 20) respiratory symptoms 
      related to cat exposure. The allergenic activity of the cat allergens was 
      investigated by loading patients' IgE onto rat basophils expressing the human 
      FcepsilonRI receptor and studying the ability of different allergen concentrations to 
      induce beta-hexosaminidase release. Purified and folded cat allergens with correct 
      masses were obtained. Cat-allergen-specific IgE levels were much higher in 
      patients with a respiratory allergy than in patients without a respiratory 
      allergy. Fel d 1, Fel d 2, Fel d 4 and Fel d 7 bound the highest levels of 
      specific IgE and already-induced basophil degranulation at hundred-fold-lower 
      concentrations than the other allergens. Fel d 1, Fel d 4 and Fel d 7 were 
      recognized by more than 65% of patients with a respiratory allergy, whereas Fel d 
      2 was recognized by only 30%. Therefore, in addition to the major cat allergen 
      Fel d 1, Fel d 4 and Fel d 7 should also be considered to be important allergens 
      for the diagnosis and specific immunotherapy of cat allergy.
FAU - Trifonova, Daria
AU  - Trifonova D
AUID- ORCID: 0009-0002-4305-0529
AD  - Division of Immunopathology, Department of Pathophysiology and Allergy Research, 
      Center for Pathophysiology, Infectiology and Immunology, Medical University of 
      Vienna, 1090 Vienna, Austria.
AD  - Laboratory of Immunopathology, Department of Clinical Immunology and Allergy, 
      Sechenov First Moscow State Medical University, 119991 Moscow, Russia.
FAU - Curin, Mirela
AU  - Curin M
AD  - Division of Immunopathology, Department of Pathophysiology and Allergy Research, 
      Center for Pathophysiology, Infectiology and Immunology, Medical University of 
      Vienna, 1090 Vienna, Austria.
FAU - Riabova, Ksenja
AU  - Riabova K
AUID- ORCID: 0000-0003-0520-0936
AD  - Laboratory of Immunopathology, Department of Clinical Immunology and Allergy, 
      Sechenov First Moscow State Medical University, 119991 Moscow, Russia.
FAU - Karsonova, Antonina
AU  - Karsonova A
AD  - Laboratory of Immunopathology, Department of Clinical Immunology and Allergy, 
      Sechenov First Moscow State Medical University, 119991 Moscow, Russia.
FAU - Keller, Walter
AU  - Keller W
AUID- ORCID: 0000-0002-2261-958X
AD  - Institute of Molecular Biosciences, BioTechMed Graz, University of Graz, 8010 
      Graz, Austria.
FAU - Gronlund, Hans
AU  - Gronlund H
AUID- ORCID: 0000-0003-4882-7624
AD  - Therapeutic Immune Design Unit, Department of Clinical Neuroscience, Karolinska 
      Institutet, 17177 Stockholm, Sweden.
FAU - Kack, Ulrika
AU  - Kack U
AD  - Department of Clinical Science and Education, Sodersjukhuset, Karolinska 
      Institutet, 11883 Stockholm, Sweden.
FAU - Konradsen, Jon R
AU  - Konradsen JR
AUID- ORCID: 0000-0001-7745-8624
AD  - Pediatric Allergy and Pulmonology, Astrid Lindgren Children's Hospital, 
      Karolinska University Hospital, 17164 Stockholm, Sweden.
AD  - Department of Women's and Children's Health, Karolinska Institutet, 17177 
      Stockholm, Sweden.
FAU - van Hage, Marianne
AU  - van Hage M
AUID- ORCID: 0000-0003-3091-1596
AD  - Division of Immunology and Allergy, Department of Medicine Solna, Karolinska 
      Institutet and University Hospital, 17177 Stockholm, Sweden.
FAU - Karaulov, Alexander
AU  - Karaulov A
AUID- ORCID: 0000-0002-1930-5424
AD  - Laboratory of Immunopathology, Department of Clinical Immunology and Allergy, 
      Sechenov First Moscow State Medical University, 119991 Moscow, Russia.
FAU - Valenta, Rudolf
AU  - Valenta R
AD  - Division of Immunopathology, Department of Pathophysiology and Allergy Research, 
      Center for Pathophysiology, Infectiology and Immunology, Medical University of 
      Vienna, 1090 Vienna, Austria.
AD  - Laboratory of Immunopathology, Department of Clinical Immunology and Allergy, 
      Sechenov First Moscow State Medical University, 119991 Moscow, Russia.
AD  - Karl Landsteiner University for Healthcare Sciences, 3500 Krems, Austria.
LA  - eng
GR  - Project no. 23-75-30016: "AllergochipRUS"/Russian Science Foundation/
PT  - Journal Article
DEP - 20231124
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Humans
MH  - Rats
MH  - Animals
MH  - Allergens/chemistry
MH  - *Hypersensitivity/diagnosis
MH  - Immunoglobulin E/metabolism
MH  - Basophils
MH  - *Asthma
PMC - PMC10706119
OTO - NOTNLM
OT  - IgE reactivity
OT  - allergenic activity
OT  - allergy
OT  - basophil activation test
OT  - cat allergen molecule
OT  - cat allergy
COIS- R.V. received research grants from HiPP GmbH & Co., Vertrieb KG, Pfaffenhofen, 
      Germany; Worg Pharmaceuticals, Hangzhou, China; HVD Biotech, Vienna, Austria; and 
      Viravaxx, Vienna, Austria. He serves as a consultant for Viravaxx and Worg. D.T., 
      M.C., K.R., A.K. (Antonina Karsonova), M.v.H., A.K. (Alexander Karaulov) and R.V. 
      are authors of the patent application "Vaccine for treating allergies" 
      application number: PCT/CN2022/130414, reference: P20222370. The other authors 
      declare no conflicts of interest. The funders had no role in the design of the 
      study; in the collection, analyses, or interpretation of data; or in the decision 
      to publish the results. The authors with Russian affiliation declare that they 
      have prepared the article in their "personal capacity" and/or that they are 
      employed at an academic/research institution where research or education is the 
      primary function of the entity.
EDAT- 2023/12/09 10:45
MHDA- 2023/12/17 09:44
PMCR- 2023/11/24
CRDT- 2023/12/09 01:14
PHST- 2023/09/28 00:00 [received]
PHST- 2023/11/03 00:00 [revised]
PHST- 2023/11/06 00:00 [accepted]
PHST- 2023/12/17 09:44 [medline]
PHST- 2023/12/09 10:45 [pubmed]
PHST- 2023/12/09 01:14 [entrez]
PHST- 2023/11/24 00:00 [pmc-release]
AID - ijms242316729 [pii]
AID - ijms-24-16729 [pii]
AID - 10.3390/ijms242316729 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Nov 24;24(23):16729. doi: 10.3390/ijms242316729.