PMID- 38071496
OWN - NLM
STAT- MEDLINE
DCOM- 20240123
LR  - 20240202
IS  - 1939-1676 (Electronic)
IS  - 0891-6640 (Print)
IS  - 0891-6640 (Linking)
VI  - 38
IP  - 1
DP  - 2024 Jan-Feb
TI  - Microarray molecular mapping of horses with severe asthma.
PG  - 477-484
LID - 10.1111/jvim.16951 [doi]
AB  - BACKGROUND: Severe asthma (SA) in horses, resembling human asthma, is a 
      prevalent, debilitating allergic respiratory condition marked by elevated 
      allergen-specific immunoglobulin E (IgE) against environmental proteins; however, 
      research exploring the exposome's influence on IgE profiles is currently limited 
      but holds paramount significance for diagnostic and therapeutic developments. 
      ANIMALS: Thirty-five sports horses were analyzed, consisting of environmentally 
      matched samples from France (5 SA; 6 control), the United States (6 SA; 6 
      control), and Canada (6 SEA; 6 control). METHODS: This intentional 
      cross-sectional study investigated the sensitization profiles of SA-affected and 
      healthy horses via serological antigen microarray profiling. Partial least 
      square-discriminant analysis (PLS-DA) was used to identify and rank the 
      importance of allergens for class separation (ie, affected/non-affected) as 
      variable influence of projection (VIP), and allergen with commonality 
      internationally established via frequency analysis. RESULTS: PLS-DA models showed 
      high discriminatory power in predicting SA in horses from Canada (area under the 
      curve [AUC] 0.995) and France (AUC 0.867) but poor discriminatory power in horses 
      from the United States (AUC 0.38). Hev b 5.0101, Cyn D, Der p 2, and Rum cr were 
      the only shared allergens across all geographical groups. CONCLUSIONS AND 
      CLINICAL IMPORTANCE: Microarray profiling can identify specific allergenic 
      components associated with SA in horses, while mathematical modeling of this data 
      can be used for disease classification, highlighting the variability of 
      sensitization profiles between geographical locations and emphasizing the 
      importance of local exposure to the prevalence of different allergens. Frequency 
      scoring analysis can identify important variables that contribute to the 
      classification of SA across different geographical regions.
CI  - (c) 2023 The Authors. Journal of Veterinary Internal Medicine published by Wiley 
      Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
FAU - White, Samuel J
AU  - White SJ
AUID- ORCID: 0000-0002-3675-7545
AD  - Nottingham Trent University, Nottinghamshire, UK.
FAU - Couetil, Laurent
AU  - Couetil L
AUID- ORCID: 0000-0002-1516-6666
AD  - Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, 
      West Lafayette, Indiana, USA.
FAU - Richard, Eric A
AU  - Richard EA
AD  - LABEO Frank Duncombe, Caen Cedex, France.
AD  - Normandie Univ, UniCaen, BIOTARGEN, Saint-Contest, France.
FAU - Marti, Eliane
AU  - Marti E
AD  - Department of Clinical Research and Veterinary Public Health, University of Bern, 
      Bern, Switzerland.
FAU - Wilson, Philippe B
AU  - Wilson PB
AUID- ORCID: 0000-0003-0207-2246
AD  - Nottingham Trent University, Nottinghamshire, UK.
LA  - eng
GR  - Medical Technologies Innovation Facility, Nottingham Trent University/
PT  - Journal Article
DEP - 20231210
PL  - United States
TA  - J Vet Intern Med
JT  - Journal of veterinary internal medicine
JID - 8708660
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Humans
MH  - Animals
MH  - Horses
MH  - Cross-Sectional Studies
MH  - *Asthma/veterinary/diagnosis
MH  - Allergens
MH  - *Hypersensitivity/veterinary
MH  - Immunoglobulin E
MH  - *Horse Diseases/diagnosis
PMC - PMC10800233
OTO - NOTNLM
OT  - IgE
OT  - One Health
OT  - allergen
OT  - microarray
OT  - severe equine asthma
COIS- Authors declare no conflict of interest.
EDAT- 2023/12/10 08:41
MHDA- 2024/01/23 06:43
PMCR- 2023/12/10
CRDT- 2023/12/10 05:28
PHST- 2023/05/08 00:00 [received]
PHST- 2023/11/08 00:00 [accepted]
PHST- 2024/01/23 06:43 [medline]
PHST- 2023/12/10 08:41 [pubmed]
PHST- 2023/12/10 05:28 [entrez]
PHST- 2023/12/10 00:00 [pmc-release]
AID - JVIM16951 [pii]
AID - 10.1111/jvim.16951 [doi]
PST - ppublish
SO  - J Vet Intern Med. 2024 Jan-Feb;38(1):477-484. doi: 10.1111/jvim.16951. Epub 2023 
      Dec 10.