PMID- 38074855
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231211
IS  - 2405-8440 (Print)
IS  - 2405-8440 (Electronic)
IS  - 2405-8440 (Linking)
VI  - 9
IP  - 11
DP  - 2023 Nov
TI  - Oral microbiota in periodontitis patients with and without type 2 diabetes 
      mellitus and their shifts after the nonsurgical periodontal therapy.
PG  - e22110
LID - 10.1016/j.heliyon.2023.e22110 [doi]
LID - e22110
AB  - OBJECTIVE: To investigate the shift in the oral microbiota of periodontitis 
      patients with and without type 2 diabetes mellitus (T2DM) undergoing nonsurgical 
      periodontal treatment and its implications. METHODS: Eleven patients with chronic 
      periodontitis and eleven patients with chronic periodontitis and diabetes from 
      the Second Affiliated Hospital of Chongqing Medical University received 
      nonsurgical periodontitis treatment and were re-evaluated 3 months later. DNA 
      from the saliva and subgingival plaques was amplified and sequenced using 16S 
      ribosomal RNA (16S rRNA) gene sequencing for microbiome profiling. Clinical 
      indexes at the onset and after periodontal therapy were recorded and compared. 
      RESULTS: The species richness and dominant microbiota of periodontitis patients 
      with and without T2DM changed significantly after nonsurgical periodontal 
      treatment in both saliva and subgingival plaques. The periodontal condition of 
      the patients was also effectively improved three months after therapy. Glycemic 
      control in patients with periodontitis and T2DM was improved. Additionally, 
      nonsurgical periodontal therapy could increase in subgingival microbial diversity 
      and the proportion of health-associated bacteria but a proportional reduction in 
      pathogenic bacteria in periodontitis patients with T2DM. Network analysis 
      revealed fewer links and a lower level of centralization in the chronic 
      periodontitis (CP) group after treatment. However, more links and a higher 
      network density of the networks were found in the CP + T2DM group, suggesting a 
      more stable microbial community after treatment. CONCLUSIONS: There were 
      significant differences in both the structural composition and reaction of the 
      oral microbiota to periodontal treatment between periodontitis patients with and 
      without T2DM. Nonsurgical periodontal treatment can improve metabolic control, 
      decrease the proportion of periodontal pathogens in oral conditions, and help 
      stabilize microbial communities in patients with periodontitis and T2DM. 
      Furthermore, nonsurgical periodontal treatment may be a potential supplementary 
      approach for managing T2DM.
CI  - (c) 2023 The Authors. Published by Elsevier Ltd.
FAU - Wang, Lan
AU  - Wang L
AD  - Department of Stomatology, The First Affiliated Hospital of Ningbo University, 
      Zhejiang, China.
FAU - Gao, Zhi
AU  - Gao Z
AD  - Department of Stomatology, The Second Affiliated Hospital of Chongqing Medical 
      University, Chongqing, China.
FAU - Zhao, Zihan
AU  - Zhao Z
AD  - Department of Stomatology, The Second Affiliated Hospital of Chongqing Medical 
      University, Chongqing, China.
FAU - Shen, Xin
AU  - Shen X
AD  - Department of Stomatology, The Second Affiliated Hospital of Chongqing Medical 
      University, Chongqing, China.
FAU - Feng, Jiali
AU  - Feng J
AD  - Stomatological Hospital of Chongqing Medical University, Chongqing, China.
FAU - Xiong, Jun
AU  - Xiong J
AD  - Department of Stomatology, The Second Affiliated Hospital of Chongqing Medical 
      University, Chongqing, China.
LA  - eng
PT  - Journal Article
DEP - 20231117
PL  - England
TA  - Heliyon
JT  - Heliyon
JID - 101672560
PMC - PMC10700393
OTO - NOTNLM
OT  - Diabetes mellitus
OT  - Oral microbiome
OT  - Periodontitis
OT  - Saliva
OT  - Subgingival plaque
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2023/12/11 06:45
MHDA- 2023/12/11 06:46
PMCR- 2023/11/17
CRDT- 2023/12/11 05:49
PHST- 2023/05/08 00:00 [received]
PHST- 2023/09/08 00:00 [revised]
PHST- 2023/11/04 00:00 [accepted]
PHST- 2023/12/11 06:46 [medline]
PHST- 2023/12/11 06:45 [pubmed]
PHST- 2023/12/11 05:49 [entrez]
PHST- 2023/11/17 00:00 [pmc-release]
AID - S2405-8440(23)09318-0 [pii]
AID - e22110 [pii]
AID - 10.1016/j.heliyon.2023.e22110 [doi]
PST - epublish
SO  - Heliyon. 2023 Nov 17;9(11):e22110. doi: 10.1016/j.heliyon.2023.e22110. 
      eCollection 2023 Nov.