PMID- 38075253
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231211
IS  - 2590-0064 (Electronic)
IS  - 2590-0064 (Linking)
VI  - 23
DP  - 2023 Dec
TI  - Effect of lyophilized gelatin-norbornene cryogel size on calvarial bone 
      regeneration.
PG  - 100868
LID - 10.1016/j.mtbio.2023.100868 [doi]
LID - 100868
AB  - Molding processes with molds containing topographical structures have been used 
      for fabrication of hydrogel and cryogel particles. However, they can involve 
      difficulties in separation of fabricated particles with complex shape from the 
      molds or repeated fabrication of the particles although the overall processes do 
      not require much skill and equipment. In this study, molds with etched 
      superhydrophobic patterns have been developed by etching polytetrafluoroethylene 
      (PTFE) blocks in user-defined designs with a femtosecond (FS) laser-based etching 
      system. Lyophilized cryogel particles with various designs and sizes were 
      fabricated by molding precursors with these PTFE molds. Additionally, the clean 
      and easy separation of particles from the molds allowed repeated fabrication of 
      the particles. For an application, relatively 'big' gelatin-norbornene (GelNB) 
      cryogel particles prepared via molding with polydimethylsiloxane (PDMS) molds, 
      swelling in phosphate buffered saline (PBS) and slicing height in half and 
      'small' GelNB cryogel particles fabricated with the PTFE molds were fabricated. 
      Then, they were used to study scaffold size effect on calvarial bone 
      regeneration. The molds generated with the FS laser-based etching system can be 
      useful for various applications that require the mass production of cryogel 
      particles in various geometries.
CI  - (c) 2023 The Authors.
FAU - Kim, Bomi
AU  - Kim B
AD  - Department of Nano-Bioengineering, Incheon National University, Incheon, 22012, 
      Republic of Korea.
FAU - Lee, Byunghak
AU  - Lee B
AD  - B2LAB Co., Ltd., Seoul, 05836, Republic of Korea.
FAU - Mandakhbayar, Nandin
AU  - Mandakhbayar N
AD  - Institute of Tissue Regeneration Engineering (ITREN), Dankook University, 
      Cheonan, 31116, Republic of Korea.
AD  - Mechanobiology Dental Medicine Research Center, Dankook University, Cheonan, 
      31116, Republic of Korea.
AD  - UCL Eastman-Korea Dental Medicine Innovation Centre, Dankook University, Cheonan, 
      31116, Republic of Korea.
AD  - Department of Biochemistry, School of Biomedicine, Mongolian National University 
      of Medical Sciences, Ulaanbaatar, 14210, Mongolia.
FAU - Kim, Younghyeon
AU  - Kim Y
AD  - B2LAB Co., Ltd., Seoul, 05836, Republic of Korea.
FAU - Song, Yeonju
AU  - Song Y
AD  - Department of Materials Science and Engineering, Seoul National University, 
      Seoul, 08826, Republic of Korea.
FAU - Doh, Junsang
AU  - Doh J
AD  - Department of Materials Science and Engineering, Seoul National University, 
      Seoul, 08826, Republic of Korea.
AD  - Institute of Engineering Research: SOFT Foundry Institute, Bio-MAX Institute, 
      Seoul National University, Seoul, 08826, Republic of Korea.
FAU - Lee, Jung-Hwan
AU  - Lee JH
AD  - Institute of Tissue Regeneration Engineering (ITREN), Dankook University, 
      Cheonan, 31116, Republic of Korea.
AD  - Mechanobiology Dental Medicine Research Center, Dankook University, Cheonan, 
      31116, Republic of Korea.
AD  - UCL Eastman-Korea Dental Medicine Innovation Centre, Dankook University, Cheonan, 
      31116, Republic of Korea.
AD  - Department of Nanobiomedical Science and BK21 NBM Global Research Center for 
      Regenerative Medicine, Dankook University, Cheonan, 31116, Republic of Korea.
AD  - Department of Biomaterials Science, College of Dentistry, Dankook University, 
      Cheonan, 31116, Republic of Korea.
AD  - Cell & Matter Institute, Dankook University, Cheonan, 31116, Republic of Korea.
FAU - Jeong, Bosu
AU  - Jeong B
AD  - B2LAB Co., Ltd., Seoul, 05836, Republic of Korea.
FAU - Song, Kwang Hoon
AU  - Song KH
AD  - Department of Nano-Bioengineering, Incheon National University, Incheon, 22012, 
      Republic of Korea.
AD  - Research Center of Brain-Machine Interface, Incheon National University, Incheon, 
      22012, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20231127
PL  - England
TA  - Mater Today Bio
JT  - Materials today. Bio
JID - 101757228
PMC - PMC10701364
OTO - NOTNLM
OT  - Calvarial bone
OT  - Lyophilized cryogel
OT  - Molding
OT  - Scaffold size
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2023/12/11 06:45
MHDA- 2023/12/11 06:46
PMCR- 2023/11/27
CRDT- 2023/12/11 05:54
PHST- 2023/09/01 00:00 [received]
PHST- 2023/10/26 00:00 [revised]
PHST- 2023/11/14 00:00 [accepted]
PHST- 2023/12/11 06:46 [medline]
PHST- 2023/12/11 06:45 [pubmed]
PHST- 2023/12/11 05:54 [entrez]
PHST- 2023/11/27 00:00 [pmc-release]
AID - S2590-0064(23)00328-9 [pii]
AID - 100868 [pii]
AID - 10.1016/j.mtbio.2023.100868 [doi]
PST - epublish
SO  - Mater Today Bio. 2023 Nov 27;23:100868. doi: 10.1016/j.mtbio.2023.100868. 
      eCollection 2023 Dec.