PMID- 38075256
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231211
IS  - 2590-0064 (Electronic)
IS  - 2590-0064 (Linking)
VI  - 23
DP  - 2023 Dec
TI  - Efficient material-induced activation of monocyte-derived dendritic cells 
      releasing surface molecules, matrix metalloproteinases, and growth factors needed 
      for regenerative tissue remodeling.
PG  - 100869
LID - 10.1016/j.mtbio.2023.100869 [doi]
LID - 100869
AB  - New experimental approaches for tissue repair have recently been proposed and 
      include the application of natural or synthetic biomaterials and immune cells. 
      Herein, fully synthetic poly(glycidyl ether) (PGE) copolymer coatings are 
      evaluated as bioinstructive materials for the in vitro culture and intrinsic 
      activation of human immune cells. Immature monocyte-derived dendritic cells 
      (moDCs) are exposed to PGE brush and gel coatings of varying copolymer 
      composition, wettability, and deformability immobilized on polystyrene culture 
      dishes. Compared to moDCs cultured on standard tissue culture-treated 
      polystyrene, activation marker levels on the cell surface are strongly enhanced 
      on PGE substrates. Thereby, moDCs undergo a distinct morphological change and 
      reach levels of activation comparable to those achieved by toll-like receptor 
      (TLR) ligand liposaccharide (LPS), specifically for the expression of 
      costimulatory molecules CD86 and CD40 as well as human leukocyte antigen 
      (HLA)-DR. In addition, PGE coatings induce a significantly enhanced level of 
      programmed cell death ligands 1 and 2 (PD-L1/-L2) on the moDC surface, two 
      molecules crucially involved in maintaining immune tolerance. In addition, an 
      increased release of matrix metalloproteinases MMP-1 and MMP-7, as well as 
      transforming growth factor (TGF)-beta1 and epidermal growth factor (EGF) was 
      observed in moDCs cultured on PGE substrates. As fully synthetic biomaterials, 
      PGE coatings demonstrate intrinsic functional competence in instructing immature 
      human moDCs for phenotypic activation in vitro, accompanied by the secretion of 
      bioactive molecules, which are known to be crucial for tissue regeneration. 
      Hence, PGE coatings hold strong potential for immune-modulating implant coatings, 
      while PGE-activated moDCs are promising candidates for future clinical cell-based 
      immunoengineering therapies.
CI  - (c) 2023 The Authors.
FAU - Stobener, Daniel David
AU  - Stobener DD
AD  - Institute of Chemistry and Biochemistry - Organic Chemistry, Freie Universitat 
      Berlin, Takustr. 3, 14195, Berlin, Germany.
AD  - Institute of Physical Chemistry and Electrochemistry, Leibniz Universitat 
      Hannover, Callinstr. 3A, 30167, Hannover, Germany.
FAU - Cosimi, Andrea
AU  - Cosimi A
AD  - Institute of Chemistry and Biochemistry - Organic Chemistry, Freie Universitat 
      Berlin, Takustr. 3, 14195, Berlin, Germany.
AD  - Institute of Physical Chemistry and Electrochemistry, Leibniz Universitat 
      Hannover, Callinstr. 3A, 30167, Hannover, Germany.
FAU - Weinhart, Marie
AU  - Weinhart M
AD  - Institute of Chemistry and Biochemistry - Organic Chemistry, Freie Universitat 
      Berlin, Takustr. 3, 14195, Berlin, Germany.
AD  - Institute of Physical Chemistry and Electrochemistry, Leibniz Universitat 
      Hannover, Callinstr. 3A, 30167, Hannover, Germany.
FAU - Peiser, Matthias
AU  - Peiser M
AD  - Institute of Chemistry and Biochemistry - Biochemistry, Freie Universitat Berlin, 
      Thielallee 63, 14195, Berlin, Germany.
LA  - eng
PT  - Journal Article
DEP - 20231115
PL  - England
TA  - Mater Today Bio
JT  - Materials today. Bio
JID - 101757228
PMC - PMC10701458
OTO - NOTNLM
OT  - Ex vivo dendritic cell priming
OT  - Functional surface coatings
OT  - Immunoengineering
OT  - Poly(glycidyl ether) copolymers
OT  - Regenerative medicine
COIS- The authors declare the following financial interests/personal relationships 
      which may be considered as potential competing interests: Marie Weinhart, 
      Matthias Peiser, and Daniel D. Stobener have filed a patent METHOD FOR ACTIVATING 
      DENDRITIC CELLS pending to Freie Universitat Berlin.
EDAT- 2023/12/11 06:45
MHDA- 2023/12/11 06:46
PMCR- 2023/11/15
CRDT- 2023/12/11 05:54
PHST- 2023/06/08 00:00 [received]
PHST- 2023/10/27 00:00 [revised]
PHST- 2023/11/14 00:00 [accepted]
PHST- 2023/12/11 06:46 [medline]
PHST- 2023/12/11 06:45 [pubmed]
PHST- 2023/12/11 05:54 [entrez]
PHST- 2023/11/15 00:00 [pmc-release]
AID - S2590-0064(23)00329-0 [pii]
AID - 100869 [pii]
AID - 10.1016/j.mtbio.2023.100869 [doi]
PST - epublish
SO  - Mater Today Bio. 2023 Nov 15;23:100869. doi: 10.1016/j.mtbio.2023.100869. 
      eCollection 2023 Dec.