PMID- 38075545
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231211
IS  - 1319-0164 (Print)
IS  - 2213-7475 (Electronic)
IS  - 1319-0164 (Linking)
VI  - 31
IP  - 12
DP  - 2023 Dec
TI  - Pharmacologically active herbal remedies against atherosclerosis, 
      characterization and DoE based marker quantification by densitometry, and cell 
      based assays on THP-1 cell lines.
PG  - 101880
LID - 10.1016/j.jsps.2023.101880 [doi]
LID - 101880
AB  - Atherosclerosis is a complex condition that develops at varying rates in multiple 
      configurations and blood vessels. The primary cause of morbidity and mortality 
      worldwide, particularly in the industrialized nations, continues to be 
      atherosclerosis. Ayurveda, Siddha, and Unani systems of medicine, among other 
      traditional medical systems, utilize polyherbal compositions. The treatment of 
      atherosclerosis has been improved with a novel multibotanical combination. In 
      this study, we sought to formulate, characterize, and standardize a polyherbal 
      formulation based on design of experiments (DoE), densitometric studies and to 
      predict for antioxidant activity using molecular docking analysis based on LC- MS 
      identified phytomarkers. In addition we have assessed its cell viability by MTT 
      assay along with Ao/EtBr staining technique and intracellular ROS assay using 
      THP-1 cell lines. Reported findings showed that the HPTLC based quantified 
      components of selected multiherbals has the ability to treat for atherosclerosis. 
      This document could be used to quickly authenticate the formulation as the method 
      optimized was based on CCD design which shows desirability of 0.962 and 0.839. 
      Cell based assays scientifically proves that the formulation was not toxic based 
      on MTT assay along with AO/EtBr staining technique and has excellent antioxidant 
      activities based on intracellular ROS assay using THP-1 cell lines. The observed 
      findings would be crucial for future clinical aspects since the bioactive 
      molecules contained in the extracts may have anticipated effects with other 
      compounds and show a superior therapeutic potential. As a result, this study 
      offers standardized and potentially therapeutic information about effective 
      polyherbal formulation for atherosclerosis.
CI  - (c) 2023 The Author(s).
FAU - Sireesha, Rayadurgam
AU  - Sireesha R
AD  - Department of Pharmaceutical Analysis, SRM College of Pharmacy, SRMIST, 
      Kattankulathur, Chengalpattu, Chennai 603203, India.
FAU - Krishnan, Manikandan
AU  - Krishnan M
AD  - Department of Pharmaceutical Analysis, SRM College of Pharmacy, SRMIST, 
      Kattankulathur, Chengalpattu, Chennai 603203, India.
FAU - Sadiq, Muhammad
AU  - Sadiq M
AD  - Molecular Biology and Immunobiology Division, Interdisciplinary Institute of 
      Indian Systems of Medicine, SRMIST, Kattankulathur, Chengalpattu, Chennai 603203, 
      India.
AD  - Department of Chemistry, SRMIST, Kattankulathur, Chengalpattu, Chennai 603203, 
      India.
FAU - Ramanujam Ganesh, Munuswamy
AU  - Ramanujam Ganesh M
AD  - Molecular Biology and Immunobiology Division, Interdisciplinary Institute of 
      Indian Systems of Medicine, SRMIST, Kattankulathur, Chengalpattu, Chennai 603203, 
      India.
AD  - Department of Chemistry, SRMIST, Kattankulathur, Chengalpattu, Chennai 603203, 
      India.
FAU - Kamaraj, Chinnaperumal
AU  - Kamaraj C
AD  - Interdisciplinary Institute of Indian System of Medicine (IIISM), Directorate of 
      Research, SRM Institute of Science and Technology (SRMIST), Kattankulathur 
      603203, Tamil Nadu, India.
FAU - Alharbi, Naiyf S
AU  - Alharbi NS
AD  - Department of Botany and Microbiology, College of Science, King Saud University, 
      P. O. Box 2455, Riyadh 11451, Saudi Arabia.
FAU - Thiruvengadam, Muthu
AU  - Thiruvengadam M
AD  - Department of Crop Science, College of Sanghuh Life Science, Konkuk University, 
      Seoul 05029, Korea.
LA  - eng
PT  - Journal Article
DEP - 20231120
PL  - Saudi Arabia
TA  - Saudi Pharm J
JT  - Saudi pharmaceutical journal : SPJ : the official publication of the Saudi 
      Pharmaceutical Society
JID - 9705695
PMC - PMC10698018
OTO - NOTNLM
OT  - AO/EtBr staining technique
OT  - Atherosclerosis
OT  - HPTLC
OT  - Intracellular ROS assay
OT  - MTT assay
OT  - Molecular docking
OT  - Multibotanicals
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2023/12/11 06:45
MHDA- 2023/12/11 06:46
PMCR- 2023/11/20
CRDT- 2023/12/11 05:58
PHST- 2023/08/30 00:00 [received]
PHST- 2023/11/17 00:00 [accepted]
PHST- 2023/12/11 06:46 [medline]
PHST- 2023/12/11 06:45 [pubmed]
PHST- 2023/12/11 05:58 [entrez]
PHST- 2023/11/20 00:00 [pmc-release]
AID - S1319-0164(23)00375-4 [pii]
AID - 101880 [pii]
AID - 10.1016/j.jsps.2023.101880 [doi]
PST - ppublish
SO  - Saudi Pharm J. 2023 Dec;31(12):101880. doi: 10.1016/j.jsps.2023.101880. Epub 2023 
      Nov 20.