PMID- 38079271
OWN - NLM
STAT- MEDLINE
DCOM- 20240118
LR  - 20240201
IS  - 1557-7430 (Electronic)
IS  - 1044-5498 (Linking)
VI  - 43
IP  - 1
DP  - 2024 Jan
TI  - Investigation on the Effect of Fluorescence Quenching of Calf Thymus DNA by 
      Piperine: Caspase Activation in the Human Breast Cancer Cell Line Studies.
PG  - 26-38
LID - 10.1089/dna.2023.0269 [doi]
AB  - In this study, we determined the interaction of piperine and calf thymus DNA (ct 
      DNA) in Tris-HCl buffer solution at pH = 6.8 and also evaluated the binding 
      mechanism through the data of multi-spectroscopic techniques along with thermal 
      melting and viscosity measurements. The outcomes of fluorescence quenching 
      confirmed the occurrence of interactions between piperine and ctDNA and pointed 
      out the role of piperine as the quencher. In addition, the K(SV) values were 
      measured at three different temperatures of 298, 303, and 308 K to be 4.5 x 10(7) 
      M(-1), 5.65 x 10(7) M(-1), and 9.36 x 10(7) M(-1), respectively, which suggested 
      the dominance of dynamic mechanism as the fluorescence quenching of 
      piperine-ctDNA. The thermodynamic parameters revealed the predominance of 
      hydrophobic forces in the interaction of ctDNA with piperine. According to the 
      resonance light scattering data, the formation of a complex between piperine and 
      ctDNA led to the creation of a larger particle. Ethidium bromide (EB) and 
      acridine orange (AO) displacement studies, along with the ionic effects of NaCl 
      and KI assessments, confirmed the interaction of piperine-ctDNA through a groove 
      binding mode. The melting temperature assay of ctDNA upon the addition of 
      piperine concentration indicated the probable groove binding of piperine to 
      ctDNA, which was affirmed by relative viscosity measurement as well. The lack of 
      detecting any alterations in the circular dichroism (CD) spectrum of CD 
      investigation verified as a characteristic sign of groove binding mechanism and 
      also confirmed all the experimental results with regard to the binding of 
      piperine-ctDNA complex. Next to observing a concentration and time-dependent 
      cytotoxicity in MDA-MB-231 cells, the impact of piperine on increasing lipid 
      peroxidation and decreasing the activity of superoxide dismutase was also 
      noticed. Apparently, piperine is capable of inducing caspase-3 activity as well.
FAU - Rezaei, Sakineh
AU  - Rezaei S
AD  - Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
FAU - Meftah, Hoda-Sadat
AU  - Meftah HS
AD  - Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
FAU - Ebtehajpour, Yasamin
AU  - Ebtehajpour Y
AD  - Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
FAU - Rahimi, Hamid Reza
AU  - Rahimi HR
AD  - Department of Medical Genetics and Molecular Medicine, School of Medicine, 
      Mashhad University of Medical Sciences, Mashhad, Iran.
FAU - Chamani, Jamshidkhan
AU  - Chamani J
AUID- ORCID: 0000-0003-2562-2458
AD  - Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
LA  - eng
PT  - Journal Article
DEP - 20231211
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 91080-16-9 (calf thymus DNA)
RN  - U71XL721QK (piperine)
RN  - 9007-49-2 (DNA)
RN  - EC 3.4.22.- (Caspases)
RN  - 0 (Alkaloids)
RN  - 0 (Piperidines)
RN  - 0 (Benzodioxoles)
RN  - 0 (Polyunsaturated Alkamides)
SB  - IM
MH  - Humans
MH  - Female
MH  - *Breast Neoplasms/drug therapy
MH  - Fluorescence
MH  - DNA/metabolism
MH  - Thermodynamics
MH  - Cell Line
MH  - Caspases
MH  - Spectrometry, Fluorescence
MH  - Molecular Docking Simulation
MH  - *Alkaloids
MH  - *Piperidines
MH  - *Benzodioxoles
MH  - *Polyunsaturated Alkamides
OTO - NOTNLM
OT  - breast cancer
OT  - calf thymus DNA
OT  - cell viability
OT  - circular dichroism
OT  - fluorescence spectroscopy
OT  - piperine
EDAT- 2023/12/11 18:43
MHDA- 2024/01/18 06:42
CRDT- 2023/12/11 12:14
PHST- 2024/01/18 06:42 [medline]
PHST- 2023/12/11 18:43 [pubmed]
PHST- 2023/12/11 12:14 [entrez]
AID - 10.1089/dna.2023.0269 [doi]
PST - ppublish
SO  - DNA Cell Biol. 2024 Jan;43(1):26-38. doi: 10.1089/dna.2023.0269. Epub 2023 Dec 
      11.