PMID- 38081259
OWN - NLM
STAT- MEDLINE
DCOM- 20231216
LR  - 20231216
IS  - 1089-7623 (Electronic)
IS  - 0034-6748 (Linking)
VI  - 94
IP  - 4
DP  - 2023 Apr 1
TI  - Development of air-assisted atomization device for the delivery of cells in 
      viscous biological ink prepared with sodium alginate.
LID - 044101 [pii]
LID - 10.1063/5.0102035 [doi]
AB  - Skin wounds, especially large-area skin trauma, would bring great pain and even 
      fatal risk to patients. In recent years, local autologous cell transplantation 
      has shown great potential for wound healing and re-epithelialization. However, 
      when the cell suspension prepared with normal saline is delivered to the wound, 
      due to its low viscosity, it is easy to form big drops in the deposition and lose 
      them from the wound bed, resulting in cell loss and uneven coverage. Here, we 
      developed a novel air-assisted atomization device (AAAD). Under proper 
      atomization parameters, 1% (w/v) sodium alginate (SA) solution carrier could be 
      sprayed uniformly. Compared with normal saline, the run-off of the SA on the 
      surface of porcine skin was greatly reduced. In theory, the spray height of AAAD 
      could be set to achieve the adjustment of a large spray area of 1-12 cm2. In the 
      measurement of droplet velocity and HaCaT cell viability, the spray height of 
      AAAD would affect the droplet settling velocity and then the cell delivery 
      survival rate (CSR). Compared with the spray height of 50 mm, the CSR of 100 mm 
      was significantly higher and could reach 91.09% +/- 1.82% (92.82% +/- 2.15% in 
      control). For bio-ink prepared with 1% (w/v) SA, the viability remained the same 
      during the 72-h incubation. Overall, the novel AAAD uniformly atomized bio-ink 
      with high viscosity and maintained the viability and proliferation rate during 
      the delivery of living cells. Therefore, AAAD has great potential in cell 
      transplantation therapy, especially for large-area or irregular skin wounds.
CI  - (c) 2023 Author(s). Published under an exclusive license by AIP Publishing.
FAU - Yan, Xintao
AU  - Yan X
AUID- ORCID: 0000-0002-2523-5063
AD  - Academy for Engineering and Technology, Fudan University, Shanghai 200433, China.
AD  - CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical 
      Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, 
      China.
FAU - Wang, Ce
AU  - Wang C
AUID- ORCID: 0000-0001-7465-796X
AD  - CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical 
      Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, 
      China.
FAU - Ma, Yuting
AU  - Ma Y
AUID- ORCID: 0000-0002-0905-6639
AD  - CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical 
      Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, 
      China.
FAU - Wang, Yao
AU  - Wang Y
AD  - CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical 
      Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, 
      China.
FAU - Song, Feifei
AU  - Song F
AD  - CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical 
      Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, 
      China.
FAU - Zhong, Jinfeng
AU  - Zhong J
AUID- ORCID: 0000-0003-4546-1049
AD  - CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical 
      Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, 
      China.
FAU - Wu, Xiaodong
AU  - Wu X
AUID- ORCID: 0000-0001-6146-9476
AD  - Academy for Engineering and Technology, Fudan University, Shanghai 200433, China.
AD  - CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical 
      Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, 
      China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Rev Sci Instrum
JT  - The Review of scientific instruments
JID - 0405571
RN  - 0 (Saline Solution)
SB  - IM
MH  - Humans
MH  - Viscosity
MH  - *Ink
MH  - *Saline Solution
MH  - Wound Healing
EDAT- 2023/12/12 00:42
MHDA- 2023/12/17 09:45
CRDT- 2023/12/11 19:13
PHST- 2022/06/06 00:00 [received]
PHST- 2023/03/19 00:00 [accepted]
PHST- 2023/12/17 09:45 [medline]
PHST- 2023/12/12 00:42 [pubmed]
PHST- 2023/12/11 19:13 [entrez]
AID - 2882900 [pii]
AID - 10.1063/5.0102035 [doi]
PST - ppublish
SO  - Rev Sci Instrum. 2023 Apr 1;94(4):044101. doi: 10.1063/5.0102035.