PMID- 38081347
OWN - NLM
STAT- MEDLINE
DCOM- 20240206
LR  - 20240209
IS  - 1096-0953 (Electronic)
IS  - 0013-9351 (Linking)
VI  - 243
DP  - 2024 Feb 15
TI  - Toxic effect of copper ions on anammox in IFFAS process filled with ZVI-10 
      modified carriers.
PG  - 117893
LID - S0013-9351(23)02697-X [pii]
LID - 10.1016/j.envres.2023.117893 [doi]
AB  - The inhibitory effects of heavy metals on anammox bacteria (AnAOB) have attracted 
      attention worldwide. However, most are conducted in activated sludge rather than 
      biofilm systems. The toxic effect and resistance response of anammox biofilm are 
      not predictable from those of free-living AnAOB. Zero valent iron (ZVI) has been 
      demonstrated to enhance anammox performance, but whether ZVI can promote AnAOB 
      resistance to heavy metal stress remains unclear. Herein, the toxic effect of 
      copper ions (Cu(II)) on anammox in integrated floating-film activated sludge 
      (IFFAS) process filled with 10 wt% ZVI modified carriers (R1) was investigated. 
      Results indicated half inhibiting concentration (IC(50)) of Cu(II) in R1 was 
      9.13 mg/L, which was much higher than that in R0 filled with conventional 
      carriers made of high density polyethylene (HDPE) (3.94 mg/L). Long-term effect 
      of Cu(II) demonstrated that Cu(II) concentrations less than 1.0 mg/L could not 
      inhibit anammox biofilm significantly, whereas R1 performed better anammox 
      process than R0 under the stress of 0.1-1.0 mg/L Cu(II). The ZVI modified 
      carriers induced more extracellular polymeric substances (EPS) to trap Cu(II) to 
      attenuate the toxicity to AnAOB. Besides, the activities of functional enzymes 
      related to anammox (NIR and HDH), as well as heme-c contents, were always higher 
      in R1 than R0 regardless of the Cu(II) dosage. Candidatus Kuenenia was identified 
      as the predominant AnAOB, which had stronger resistance to Cu(II) stress compared 
      to other genera in the IFFAS process. Metal resistance genes (MRGs) analysis 
      identified AnAOB induced multi-responses to resist Cu(II) stress, such as the 
      up-regulation of copC, cutA, cutC, cutF, cueR and cueO, to synthesize more 
      proteins with functions of copper exocytosis, conjugation and oxidation.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Liu, Tao
AU  - Liu T
AD  - Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of 
      Education), School of Environmental Science and Technology, Dalian University of 
      Technology, Dalian, 116024, China. Electronic address: taoliu@dlut.edu.cn.
FAU - Li, Chaohui
AU  - Li C
AD  - Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of 
      Education), School of Environmental Science and Technology, Dalian University of 
      Technology, Dalian, 116024, China.
FAU - Quan, Xie
AU  - Quan X
AD  - Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of 
      Education), School of Environmental Science and Technology, Dalian University of 
      Technology, Dalian, 116024, China.
LA  - eng
PT  - Journal Article
DEP - 20231210
PL  - Netherlands
TA  - Environ Res
JT  - Environmental research
JID - 0147621
RN  - 0 (Sewage)
RN  - 789U1901C5 (Copper)
RN  - E1UOL152H7 (Iron)
RN  - 0 (Metals, Heavy)
RN  - 0 (Ions)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - *Sewage/microbiology
MH  - Copper/toxicity
MH  - Iron
MH  - Anaerobic Ammonia Oxidation
MH  - *Metals, Heavy
MH  - Bacteria/metabolism
MH  - Oxidation-Reduction
MH  - Ions
MH  - Bioreactors/microbiology
MH  - Nitrogen/analysis
OTO - NOTNLM
OT  - Anammox
OT  - Copper ions
OT  - MRG response
OT  - Resistance
OT  - ZVI-10 modified carrier
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/12/12 00:42
MHDA- 2024/02/06 06:42
CRDT- 2023/12/11 19:22
PHST- 2023/11/03 00:00 [received]
PHST- 2023/12/06 00:00 [revised]
PHST- 2023/12/06 00:00 [accepted]
PHST- 2024/02/06 06:42 [medline]
PHST- 2023/12/12 00:42 [pubmed]
PHST- 2023/12/11 19:22 [entrez]
AID - S0013-9351(23)02697-X [pii]
AID - 10.1016/j.envres.2023.117893 [doi]
PST - ppublish
SO  - Environ Res. 2024 Feb 15;243:117893. doi: 10.1016/j.envres.2023.117893. Epub 2023 
      Dec 10.