PMID- 38088826 OWN - NLM STAT- MEDLINE DCOM- 20231216 LR - 20231216 IS - 1552-5783 (Electronic) IS - 0146-0404 (Print) IS - 0146-0404 (Linking) VI - 64 IP - 15 DP - 2023 Dec 1 TI - Association of Circulating Antiretinal Antibodies With Clinical Outcomes in Retinitis Pigmentosa. PG - 13 LID - 10.1167/iovs.64.15.13 [doi] LID - 13 AB - PURPOSE: To determine if circulating antiretinal antibodies (ARAs) differ between patients affected by retinitis pigmentosa (RP) and control participants and to assess whether ARAs are associated with clinical outcomes in patients with RP. METHODS: Cross-sectional study involving a group of patients clinically diagnosed with RP and a control group of healthy participants. Serum autoantibodies against enolase, heat shock protein 70 (HSP70), and carbonic anhydrase II (CAII) were tested in all participants using Jess capillary Western blot. We compared ARA prevalence between the RP and control groups and investigated the association of serum ARA positivity with macular edema and vitreomacular disorders in patients affected by RP. RESULTS: Thirty-six patients affected by RP and a control group of 39 healthy individuals were included. Overall, at least one ARA positivity was detected in 89% and 80% of participants in the RP and control groups, respectively. We observed a similar prevalence of anti-CAII and anti-enolase ARA between patients and controls (P = 0.87 and P = 0.35, respectively). Sera from patients with RP tested positive for anti-HSP70 ARAs more frequently than those from controls (53% vs. 36%), albeit without reaching statistical significance (P = 0.29). Among the 72 eyes with RP, 25% presented with macular edema (most often bilateral) and 33% with epiretinal membrane and/or lamellar macular hole. None of the three ARAs was associated with an increased risk of any macular complications in eyes affected by RP (all P > 0.05). CONCLUSIONS: The prevalence of circulating ARAs against enolase, HSP70, and CAII is similar between patients affected by RP and healthy individuals. Our results provide evidence against the association of ARAs with macular edema and vitreomacular interface disorders in RP. FAU - Bianco, Lorenzo AU - Bianco L AD - Department of Ophthalmology, IRCCS San Raffaele Scientific Institute, Milan, Italy. AD - University Vita-Salute San Raffaele, Milan, Italy. FAU - Arrigo, Alessandro AU - Arrigo A AD - Department of Ophthalmology, IRCCS San Raffaele Scientific Institute, Milan, Italy. AD - University Vita-Salute San Raffaele, Milan, Italy. FAU - Antropoli, Alessio AU - Antropoli A AD - Department of Ophthalmology, IRCCS San Raffaele Scientific Institute, Milan, Italy. AD - University Vita-Salute San Raffaele, Milan, Italy. FAU - Del Fabbro, Sebastiano AU - Del Fabbro S AD - Department of Ophthalmology, IRCCS San Raffaele Scientific Institute, Milan, Italy. AD - University Vita-Salute San Raffaele, Milan, Italy. FAU - Panina-Bordignon, Paola AU - Panina-Bordignon P AD - University Vita-Salute San Raffaele, Milan, Italy. AD - Neuroimmunology Unit, Institute of Experimental Neurology, IRCCS San Raffaele Scientific Institute, Milan, Italy. FAU - Peri, Carolina AU - Peri C AD - Neuroimmunology Unit, Institute of Experimental Neurology, IRCCS San Raffaele Scientific Institute, Milan, Italy. FAU - Brambilla, Elena AU - Brambilla E AD - University Vita-Salute San Raffaele, Milan, Italy. AD - Neuroimmunology Unit, Institute of Experimental Neurology, IRCCS San Raffaele Scientific Institute, Milan, Italy. FAU - Pina, Adelaide AU - Pina A AD - Department of Ophthalmology, IRCCS San Raffaele Scientific Institute, Milan, Italy. FAU - Basile, Giulia AU - Basile G AD - Department of Ophthalmology, IRCCS San Raffaele Scientific Institute, Milan, Italy. FAU - Hassan Farah, Rashid AU - Hassan Farah R AD - Department of Ophthalmology, IRCCS San Raffaele Scientific Institute, Milan, Italy. FAU - Saladino, Andrea AU - Saladino A AD - Department of Ophthalmology, IRCCS San Raffaele Scientific Institute, Milan, Italy. AD - University Vita-Salute San Raffaele, Milan, Italy. FAU - Aragona, Emanuela AU - Aragona E AD - Department of Ophthalmology, IRCCS San Raffaele Scientific Institute, Milan, Italy. AD - University Vita-Salute San Raffaele, Milan, Italy. FAU - Cascavilla, Maria Lucia AU - Cascavilla ML AD - Department of Ophthalmology, IRCCS San Raffaele Scientific Institute, Milan, Italy. AD - University Vita-Salute San Raffaele, Milan, Italy. FAU - Bandello, Francesco AU - Bandello F AD - Department of Ophthalmology, IRCCS San Raffaele Scientific Institute, Milan, Italy. AD - University Vita-Salute San Raffaele, Milan, Italy. FAU - Battaglia Parodi, Maurizio AU - Battaglia Parodi M AD - Department of Ophthalmology, IRCCS San Raffaele Scientific Institute, Milan, Italy. AD - University Vita-Salute San Raffaele, Milan, Italy. FAU - Pulido, Jose S AU - Pulido JS AD - Larry Donoso Chair of Translational Ophthalmology, Wills Eye Hospital, Philadelphia, Pennsylvania, United States. LA - eng PT - Journal Article PL - United States TA - Invest Ophthalmol Vis Sci JT - Investigative ophthalmology & visual science JID - 7703701 RN - EC 4.2.1.11 (Phosphopyruvate Hydratase) SB - IM MH - Humans MH - *Macular Edema/diagnosis/etiology MH - Cross-Sectional Studies MH - *Retinitis Pigmentosa/diagnosis/complications MH - Retina MH - Phosphopyruvate Hydratase MH - Tomography, Optical Coherence/methods PMC - PMC10720755 COIS- Disclosure: L. Bianco, None; A. Arrigo, None; A. Antropoli, None; S. Del Fabbro, None; P. Panina-Bordignon, None; C. Peri, None; E. Brambilla, None; A. Pina, None; G. Basile, None; R. Hassan Farah, None; A. Saladino, None; E. Aragona, None; M.L. Cascavilla, None; F. Bandello, None; M. Battaglia Parodi, None; J.S. Pulido, None EDAT- 2023/12/13 12:43 MHDA- 2023/12/17 13:19 PMCR- 2023/12/13 CRDT- 2023/12/13 10:33 PHST- 2023/12/17 13:19 [medline] PHST- 2023/12/13 12:43 [pubmed] PHST- 2023/12/13 10:33 [entrez] PHST- 2023/12/13 00:00 [pmc-release] AID - 2793104 [pii] AID - IOVS-23-37721 [pii] AID - 10.1167/iovs.64.15.13 [doi] PST - ppublish SO - Invest Ophthalmol Vis Sci. 2023 Dec 1;64(15):13. doi: 10.1167/iovs.64.15.13.