PMID- 38092323
OWN - NLM
STAT- MEDLINE
DCOM- 20240122
LR  - 20240201
IS  - 1089-8646 (Electronic)
IS  - 0888-7543 (Linking)
VI  - 116
IP  - 1
DP  - 2024 Jan
TI  - A novel link between circPDE3B and ferroptosis in esophageal squamous cell 
      carcinoma progression.
PG  - 110761
LID - S0888-7543(23)00205-7 [pii]
LID - 10.1016/j.ygeno.2023.110761 [doi]
AB  - AIM: To unravel whether ferroptosis involves with the actions by 
      circPDE3B-mediated facilitation of esophageal squamous cell carcinoma (ESCC) 
      progression. METHODS: Human ESCC tissues and cell lines were prepared for the 
      evaluation of ferroptosis. Cellular iron, ROS, GSH, and MDA levels were measured 
      to assess ferroptosis. Flow cytometry was employed to analyze apoptosis and cell 
      cycle. Subcellular fractionation and fluorescence in situ hybridization (FISH) 
      were conducted to validate the localization of circPDE3B. RNA pull-down, RNA 
      immunoprecipitation (RIP), and luciferase assay were subjected to identify the 
      molecular mechanisms. Nude mouse xenograft model was carried out to evaluate the 
      function of circPDE3B/SLC7A11/CBS in vivo. RESULTS: Increased circPDE3B in human 
      ESCC specimens was positively correlated with ferroptosis-related molecules, 
      SLC7A11 and CBS. Functionally, circPDE3B knockdown triggered ferroptosis, 
      apoptosis, and cell cycle arrest in ESCC cells. Whereas, these effects were 
      obviously blocked by miR-516b-5p inhibitor. Mechanistically, not only circPDE3B 
      sponged miR-516b-5p to upregulate CBS, but also directly bound with HNRNPK to 
      stabilize SLC7A11. In mice, depletion of circPDE3B restrained ESCC growth, while 
      this was abolished by overexpression of CBS or SLC7A11. CONCLUSION: In summary, 
      circPDE3B promotes ESCC progression by suppressing ferroptosis through recruiting 
      HNRNPK/SLC7A11 and miR-516b-5p/CBS axes.
CI  - Copyright (c) 2023. Published by Elsevier Inc.
FAU - Zhou, Pengli
AU  - Zhou P
AD  - Intervention Department, the First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450052, Henan Province, PR China.
FAU - Wu, Zhengyang
AU  - Wu Z
AD  - Intervention Department, the First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450052, Henan Province, PR China.
FAU - Zhang, Qinghui
AU  - Zhang Q
AD  - Intervention Department, the First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450052, Henan Province, PR China.
FAU - Wang, Ling
AU  - Wang L
AD  - Intervention Department, the First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450052, Henan Province, PR China.
FAU - Zhang, Wenguang
AU  - Zhang W
AD  - Intervention Department, the First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450052, Henan Province, PR China.
FAU - Han, Xinwei
AU  - Han X
AD  - Intervention Department, the First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450052, Henan Province, PR China. Electronic address: 
      hanxinwei2006@163.com.
LA  - eng
PT  - Journal Article
DEP - 20231211
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Humans
MH  - Animals
MH  - Mice
MH  - *Esophageal Squamous Cell Carcinoma/pathology
MH  - *Esophageal Neoplasms/pathology
MH  - *MicroRNAs/genetics/metabolism
MH  - *Ferroptosis/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Cell Proliferation/genetics
MH  - Cell Line, Tumor
MH  - Gene Expression Regulation, Neoplastic
OTO - NOTNLM
OT  - CBS
OT  - CircPDE3B
OT  - Esophageal squamous cell carcinoma
OT  - Ferroptosis
OT  - HNRNPK
OT  - MiR-516b-5p
OT  - SLC7A11
COIS- Declaration of Competing Interest The authors declare no competing financial 
      interests.
EDAT- 2023/12/14 00:42
MHDA- 2024/01/22 06:41
CRDT- 2023/12/13 19:29
PHST- 2023/07/14 00:00 [received]
PHST- 2023/10/30 00:00 [revised]
PHST- 2023/12/10 00:00 [accepted]
PHST- 2024/01/22 06:41 [medline]
PHST- 2023/12/14 00:42 [pubmed]
PHST- 2023/12/13 19:29 [entrez]
AID - S0888-7543(23)00205-7 [pii]
AID - 10.1016/j.ygeno.2023.110761 [doi]
PST - ppublish
SO  - Genomics. 2024 Jan;116(1):110761. doi: 10.1016/j.ygeno.2023.110761. Epub 2023 Dec 
      11.