PMID- 38096019
OWN - NLM
STAT- MEDLINE
DCOM- 20231216
LR  - 20231216
IS  - 2050-084X (Electronic)
IS  - 2050-084X (Linking)
VI  - 12
DP  - 2023 Dec 14
TI  - Postsynaptic cell type and synaptic distance do not determine efficiency of 
      monosynaptic rabies virus spread measured at synaptic resolution.
LID - 10.7554/eLife.89297 [doi]
LID - e89297
AB  - Retrograde monosynaptic tracing using glycoprotein-deleted rabies virus is an 
      important component of the toolkit for investigation of neural circuit structure 
      and connectivity. It allows for the identification of first-order presynaptic 
      connections to cell populations of interest across both the central and 
      peripheral nervous system, helping to decipher the complex connectivity patterns 
      of neural networks that give rise to brain function. Despite its utility, the 
      factors that influence the probability of transsynaptic rabies spread are not 
      well understood. While it is well established that expression levels of rabies 
      glycoprotein used to trans-complement G-deleted rabies can result in large 
      changes in numbers of inputs labeled per starter cell (convergence index [CI]), 
      it is not known how typical values of CI relate to the proportions of synaptic 
      contacts or input neurons labeled. And it is not known whether inputs to 
      different cell types, or synaptic contacts that are more proximal or distal to 
      the cell body, are labeled with different probabilities. Here, we use a new 
      rabies virus construct that allows for the simultaneous labeling of pre- and 
      postsynaptic specializations to quantify the proportion of synaptic contacts 
      labeled in mouse primary visual cortex. We demonstrate that with typical 
      conditions about 40% of first-order presynaptic excitatory synapses to cortical 
      excitatory and inhibitory neurons are labeled. We show that using matched tracing 
      conditions there are similar proportions of labeled contacts onto L4 excitatory 
      pyramidal, somatostatin (Sst) inhibitory, and vasoactive intestinal peptide (Vip) 
      starter cell types. Furthermore, we find no difference in the proportions of 
      labeled excitatory contacts onto postsynaptic sites at different subcellular 
      locations.
CI  - (c) 2023, Patino et al.
FAU - Patino, Maribel
AU  - Patino M
AUID- ORCID: 0000-0003-0820-3315
AD  - Systems Neurobiology Laboratories, The Salk Institute for Biological Studies, La 
      Jolla, United States.
AD  - Neuroscience Graduate Program, University of California, San Diego, La Jolla, 
      United States.
AD  - Medical Scientist Training Program, University of California, San Diego, La 
      Jolla, United States.
FAU - Lagos, Willian N
AU  - Lagos WN
AD  - Systems Neurobiology Laboratories, The Salk Institute for Biological Studies, La 
      Jolla, United States.
FAU - Patne, Neelakshi S
AU  - Patne NS
AD  - Systems Neurobiology Laboratories, The Salk Institute for Biological Studies, La 
      Jolla, United States.
FAU - Miyazaki, Paula A
AU  - Miyazaki PA
AD  - Systems Neurobiology Laboratories, The Salk Institute for Biological Studies, La 
      Jolla, United States.
FAU - Bhamidipati, Sai Krishna
AU  - Bhamidipati SK
AUID- ORCID: 0000-0003-3498-3730
AD  - Systems Neurobiology Laboratories, The Salk Institute for Biological Studies, La 
      Jolla, United States.
FAU - Collman, Forrest
AU  - Collman F
AUID- ORCID: 0000-0002-0280-7022
AD  - Allen Institute for Brain Science, Seattle, United States.
FAU - Callaway, Edward M
AU  - Callaway EM
AUID- ORCID: 0000-0002-6366-5267
AD  - Systems Neurobiology Laboratories, The Salk Institute for Biological Studies, La 
      Jolla, United States.
LA  - eng
GR  - R01 EY022577/EY/NEI NIH HHS/United States
GR  - T32 GM007198/GM/NIGMS NIH HHS/United States
GR  - U24 NS120053/NS/NINDS NIH HHS/United States
GR  - U24NS120053/NS/NINDS NIH HHS/United States
PT  - Journal Article
DEP - 20231214
PL  - England
TA  - Elife
JT  - eLife
JID - 101579614
RN  - 0 (Glycoproteins)
SB  - IM
UOF - doi: 10.1101/2022.08.31.506012
MH  - Mice
MH  - Animals
MH  - *Rabies virus
MH  - *Rabies
MH  - Neurons/physiology
MH  - Synapses/physiology
MH  - Glycoproteins/metabolism
PMC - PMC10721217
OTO - NOTNLM
OT  - cerebral cortex
OT  - interneuron
OT  - mouse
OT  - neural circuit
OT  - neuroscience
OT  - rabies virus
OT  - synapse
COIS- MP, WL, NP, PM, SB, FC, EC No competing interests declared
EDAT- 2023/12/14 12:42
MHDA- 2023/12/17 09:46
PMCR- 2023/12/14
CRDT- 2023/12/14 11:54
PHST- 2023/05/11 00:00 [received]
PHST- 2023/11/19 00:00 [accepted]
PHST- 2023/12/17 09:46 [medline]
PHST- 2023/12/14 12:42 [pubmed]
PHST- 2023/12/14 11:54 [entrez]
PHST- 2023/12/14 00:00 [pmc-release]
AID - 89297 [pii]
AID - 10.7554/eLife.89297 [doi]
PST - epublish
SO  - Elife. 2023 Dec 14;12:e89297. doi: 10.7554/eLife.89297.